{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T13:59:23Z","timestamp":1792504763157,"version":"4.5.0"},"reference-count":60,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["451-03-68\/2020-14\/200114"],"award-info":[{"award-number":["451-03-68\/2020-14\/200114"]}]},{"name":"Slovenian Agency for Research and Innovation","award":["P4-0116"],"award-info":[{"award-number":["P4-0116"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Aromatic plants like peppermint (Mentha x piperita, Lamiaceae) have a long tradition of use. Most of the plant material is used to produce herbal drugs and for the isolation of essential oils. However, since essential oils are present in very small amounts, the largest proportion of plants remains unused. Objectives: The aims of this study were the analysis of chemical, biochemical, antimicrobial, and pharmacological properties of peppermint waste material extracts (derived from stems, post-distillation waste, and deodorized leaves) in comparison with the officially prepared extract. Results: The obtained results revealed that the investigated peppermint waste extracts (PWEs) are a rich source of phenolic compounds, where rosmarinic acid was determined as the dominant one (7.05\u201321.19 mg\/g d.e.). Antioxidant potential and hepatoprotective effect of PWE were comparable with the official extract, where the most active ones were those prepared by treating the deodorized leaves with both 45% and 75% ethanol. In addition, PWE exhibited notable antimicrobial and anticholinesterase activity. Results of pharmacological studies on experimental animals showed that peppermint extracts (official and those made from deodorized leaves) did not interfere with the effect of the tested drugs, midazolam and fluoxetine. The examined extracts neither exerted an influence on motor coordination nor acted as antidepressants. Results of the elevated plus maze test indicated that PWE affected the activity of the central nervous system. Conclusions: PWEs represent a significant source of phenolic compounds, especially rosmarinic acid, and they can be used in the pharmaceutical industry to produce various herbal products and in the food industry as natural additives.<\/jats:p>","DOI":"10.3390\/ph18121782","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T10:24:17Z","timestamp":1763979857000},"page":"1782","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chemical, Biochemical, Antimicrobial, and Pharmacological Assessment of Postdistillation Waste Material Extracts of Mentha x piperita"],"prefix":"10.3390","volume":"18","author":[{"given":"Neda","family":"Gavari\u0107","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"Radovanovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5286-3858","authenticated-orcid":false,"given":"Nata\u0161a","family":"Milo\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0540-6654","authenticated-orcid":false,"given":"Jelena","family":"Jovi\u010di\u0107-Bata","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8354-3868","authenticated-orcid":false,"given":"Mladena","family":"Lali\u0107-Popovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7949-8128","authenticated-orcid":false,"given":"Sonja Smole","family":"Mo\u017eina","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1081-6666","authenticated-orcid":false,"given":"Isidora","family":"Samojlik","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Silva, H. (2020). A Descriptive Overview of the Medical Uses Given to Mentha Aromatic Herbs throughout History. Biology, 9.","DOI":"10.3390\/biology9120484"},{"key":"ref_2","unstructured":"Yousefian, S., Esmaeili, F., and Lohrasebi, T. (2023). A Comprehensive Review of the Key characteristics of the Genus Mentha, Natural Compounds and Biotechnological Approaches for the Production of Secondary Metabolites. Iran. J. Biotechnol., 21."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Takhtajan, A. (2009). Flowering Plants, Springer.","DOI":"10.1007\/978-1-4020-9609-9"},{"key":"ref_4","unstructured":"European Medicines Agency (EMEA) (2025, June 08). Community Herbal Monograph on Mentha \u00d7 piperita L., folium. Available online: https:\/\/www.ema.europa.eu\/en\/documents\/herbal-monograph\/european-union-herbal-monograph-mentha-x-piperita-l-folium-revision-1_en.pdf."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.sajb.2021.05.014","article-title":"Mentha piperita phytochemicals in agriculture, food industry and medicine: Features and applications","volume":"141","author":"Gholamipourfard","year":"2021","journal-title":"S. Afr. J. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3141","DOI":"10.2174\/138161208786404245","article-title":"Mentha L. Species (Lamiaceae) as promising sources of bioactive secondary metabolites","volume":"14","year":"2008","journal-title":"Curr. Pharm. Des."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Hudz, N., Kobylinska, L., Pokajewicz, K., Horcinov\u00e1 Sedl\u00e1ckov\u00e1, V., Fedin, R., Voloshyn, M., Myskiv, I., Brindza, J., Wieczorek, P., and Lipok, J. (2023). Mentha piperita: Essential Oil and Extracts, Their Biological Activities, and Perspectives on the Development of New Medicinal and Cosmetic Products. Molecules, 28.","DOI":"10.3390\/molecules28217444"},{"key":"ref_8","unstructured":"European Pharmacopoea (2019). Monographs on Herbal Drugs and Herbal Drug Preparations, European Directorate for the Quality of Medicines & Health Care, Council of Europe. [10th ed.]."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Voigt, V., Franke, H., and Lachenmeier, D. (2024). Risk Assessment of Pulegone in Foods Based on Benchmark Dose\u2013Response Modeling. Foods, 13.","DOI":"10.3390\/foods13182906"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sadowska, U., Villavicencio, R.A., Dziadek, K., Skoczylas, J., Sadowski, S.K., and Kopec, A. (2024). The Identification of Polyphenolic Compounds and the Determination of Antioxidant Activity in Extracts and Infusions of Peppermint, Lemon Balm and Lavender. Appl. Sci., 14.","DOI":"10.3390\/app14020699"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"133","DOI":"10.4103\/japtr.japtr_16_22","article-title":"Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract","volume":"13","author":"Rosmalena","year":"2022","journal-title":"J. Adv. Pharm. Technol. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Elansary, H.O., Szopa, A., Kubica, P., Ekiert, H., Klimek-Szczykutowicz, M., El-Ansary, D.O., and Mahmoud, E.A. (2020). Polyphenol Profile and Antimicrobial and Cytotoxic Activities of Natural Mentha \u00d7 piperita and Mentha longifolia Populations in Northern Saudi Arabia. Processes, 8.","DOI":"10.3390\/pr8040479"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.sajb.2010.05.011","article-title":"Peppermint (Mentha piperita) inhibits microbial biofilms in vitro","volume":"77","author":"Sandasi","year":"2011","journal-title":"S. Afr. J. Bot."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.arabjc.2011.01.019","article-title":"Antibacterial and antioxidant activity of Mentha piperita","volume":"8","author":"Singh","year":"2015","journal-title":"Arab. J. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1007\/s10068-017-0217-9","article-title":"In vitro antiviral, anti-inflammatory, and antioxidant activities of the ethanol extract of Mentha piperita L.","volume":"26","author":"YuXian","year":"2017","journal-title":"Food Sci. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1134\/S1068162022070238","article-title":"Anti-Influenza activity of extracts from plants of the Lamiaceae familx","volume":"48","author":"Protsenko","year":"2022","journal-title":"Russ. J. Bioorg. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.indcrop.2011.02.019","article-title":"Insecticidal properties of Mentha species: A review","volume":"34","author":"Kumar","year":"2011","journal-title":"Ind. Crops Prod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1177\/1091581810390527","article-title":"Evaluation of cytotoxicity and anticarcinogenesis potential of Mentha leaf extracts","volume":"30","author":"Jain","year":"2011","journal-title":"Int. J. Toxicol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1002\/ptr.3638","article-title":"Acute and chronic pretreatment with essential oil of peppermint (Mentha \u00d7 piperita L., Lamiaceae) influences drug effects","volume":"26","author":"Samojlik","year":"2012","journal-title":"Phytother. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/jcpt.12679","article-title":"The role and mechanism of action of menthol in topical analgesic products","volume":"43","author":"Pergolizzi","year":"2018","journal-title":"J. Clin. Pharm. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Anjum, R., Raza, C., Faheem, M., Ullah, A., and Chaudhry, M. (2024). Neuroprotective potential of Mentha piperita extract prevents motor dysfunctions in mouse model of Parkinson\u2019s disease through anti-oxidant capacities. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0302102"},{"key":"ref_22","unstructured":"Food and Drug Administration, Department of Health and Human Services (2025, September 21). Spices and Other Natural Seasonings and Flavorings, Available online: https:\/\/www.ecfr.gov\/current\/title-21\/part-182\/section-182.10."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.foodchem.2004.06.029","article-title":"Effect of acid treatment on the phytochemical and antioxidant characteristics of extracts from selected Lamiaceae species","volume":"91","author":"Kosar","year":"2005","journal-title":"Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Patra, A., Abdullah, S., and Pradhan, R.C. (2022). Review on the extraction of bioactive compounds and characterization of fruit industry by-products. Bioresour. Bioprocess., 9.","DOI":"10.1186\/s40643-022-00498-3"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4282","DOI":"10.1007\/s13197-020-04903-7","article-title":"Peppermint leaves hydrodistillation by-products: Bioactive properties and incorporation into icecream formulations","volume":"58","author":"Berktas","year":"2020","journal-title":"J. Food Sci. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4563","DOI":"10.1021\/jf034108k","article-title":"Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varietes and cultivars","volume":"52","author":"Dorman","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5004","DOI":"10.1021\/jf0496189","article-title":"Screening of free radical scavenging compounds in water extracts of Mentha samples using a postcolumn derivatization method","volume":"52","author":"Kosar","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_28","first-page":"535","article-title":"Phenolic profile and antioxidant evaluation of Mentha \u00d7 piperita (Peppermint) extracts","volume":"4","author":"Dorman","year":"2009","journal-title":"Nat. Prod. Comm."},{"key":"ref_29","unstructured":"Gavari\u0107, N., Kladar, N., Bogavac, M., Mijatovi\u0107 Jovin, V., Samojlik, I., and Bo\u017ein, B. (2015). Anxyolitic effect of deodorised extracts of Mentha \u00d7 piperita L., Lamiaceae. Abstract Book, The 15th International Congress of the International Society for Ethno-Pharmacology, Petra, Jordan, 5\u20138 May 2015, International Society for Ethno-Pharmacology."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Guan, H., Luo, W., Bao, B., Cao, Y., Cheng, F., Yu, S., Fan, Q., Zhang, L., Wu, Q., and Shan, M. (2022). A comprehensive review of rosmarinic acid: From phytochemistry to pharmacology and its new insight. Molecules, 27.","DOI":"10.3390\/molecules27103292"},{"key":"ref_31","first-page":"559","article-title":"LC-MS analysis, antioxidant and antimicrobial activity for five species of Mentha cultivated in Romania","volume":"9","author":"Moldovan","year":"2014","journal-title":"Dig. J. Nanomater. Biostruct."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3945\/an.116.012948","article-title":"Flavones: Food Sources, bioavailability, metabolism, and bioactivity","volume":"8","author":"Hostetler","year":"2017","journal-title":"Adv. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Knez, E., Kadac-Czapska, K., and Grembecka, M. (2025). Evaluation of spectrophotometric methods for assessing antioxidant potential in plant food samples\u2014A critical approach. Appl. Sci., 15.","DOI":"10.3390\/app15115925"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1007\/s00204-023-03562-9","article-title":"Reactive oxygen species, toxicity, oxidative stress, and antioxidants: Chronic disease and aging","volume":"97","author":"Jomova","year":"2023","journal-title":"Arch. Toxicol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e10467","DOI":"10.1016\/j.heliyon.2022.e10467","article-title":"Structure-antiradical activity relationships of 25 natural antioxidant phenolic compounds from different classes","volume":"8","author":"Moazzen","year":"2022","journal-title":"Heliyon"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0308-8146(03)00088-8","article-title":"Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs","volume":"83","author":"Dorman","year":"2003","journal-title":"Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1002\/ptr.3366","article-title":"Infusions and decoctions of mixed herbs used in folk medicine: Synergism of antioxidant potential","volume":"25","author":"Guimares","year":"2011","journal-title":"Phytother. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.ijfoodmicro.2012.03.006","article-title":"Plant extracts for the control of bacterial growth: Efficacy, stability and safety for food application","volume":"156","author":"Negi","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100184","DOI":"10.1016\/j.phyplu.2021.100184","article-title":"Advances on plant extracts and phytocompounds with acetylcholinesterase inhibition activity for possible treatment of Alzheimer\u2019s disease","volume":"2","author":"Taqui","year":"2022","journal-title":"Phytomed. Plus."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"767","DOI":"10.3390\/molecules19010767","article-title":"Acetylcholinesterase inhibitory, antioxidant and phytochemical properties of selected medicinal plants of the Lamiaceae family","volume":"19","author":"Kindl","year":"2014","journal-title":"Molecules"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Srief, M., Moustafa, B., Mokrani, E.H., Mennai, I., Hamdi, M., Boumechhour, A., Mustapha, M.A., Derdour, M., Kerkatou, M., and El-Shazly, M. (2023). Evaluation on in vitro and in silico anti-alzheimer potential of nonpolar extracts and essential oil from Mentha piperita. Foods, 12.","DOI":"10.3390\/foods12010190"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.phrs.2019.01.028","article-title":"Interaction of herbal products with prescribed medications: A systematic review and meta-analysis","volume":"141","author":"Awortwe","year":"2019","journal-title":"Pharmacol. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.sajb.2024.07.033","article-title":"Can we use Melissa officinalis (lemon balm) postdistillation waste extracts in pharmacy? In vivo pharmacodynamic studies","volume":"172","author":"Kladar","year":"2024","journal-title":"S. Afr. J. Bot."},{"key":"ref_44","first-page":"44","article-title":"The aqueous extract of Mentha piperita (Peppermint) can alter depression parameters: A behavioral study in mice","volume":"4","author":"Moghari","year":"2023","journal-title":"Avicenna J. Pharm. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/nprot.2007.44","article-title":"The use of elevated plus maze as an assay of anxiety-related behavior in rodents","volume":"2","author":"Walf","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"431","DOI":"10.2174\/1570159X18666200106155127","article-title":"Neuroprotective Polyphenols: A Modulatory Action on Neurotransmitter Pathways","volume":"18","author":"Rebas","year":"2020","journal-title":"Curr. Neuropharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.indcrop.2015.05.070","article-title":"Postdistillation waste material of thyme (Thymus vulgaris L., Lamiaceae) as a potential source of biologically active compounds","volume":"74","author":"Kladar","year":"2015","journal-title":"Ind. Crops Prod."},{"key":"ref_48","first-page":"133","article-title":"Development of a rapid resolution HPLC method for the separation and determination of 17 phenolic compounds in crude plant extracts","volume":"9","author":"Sedej","year":"2011","journal-title":"Centr. Eur. J. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/j.foodchem.2008.04.071","article-title":"Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae)","volume":"111","author":"Samojlik","year":"2008","journal-title":"Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.foodchem.2008.06.026","article-title":"Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method","volume":"112","author":"Scherer","year":"2009","journal-title":"Food Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1021\/jf051922u","article-title":"Characterization of the volatile composition of essential oils of some Lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils","volume":"54","author":"Simin","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.colsurfb.2010.08.012","article-title":"Solubilization of fullerene C60 in micellar solutions of different solubilizers","volume":"82","author":"Simplici","year":"2011","journal-title":"Coll. Surf. B"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"8848","DOI":"10.1021\/jf101645n","article-title":"Antioxidant and Hepatoprotective Potential of Essential Oils of Coriander (Coriandrum sativum L.) and Caraway (Carum carvi L.) (Apiaceae)","volume":"58","author":"Samojlik","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Gavari\u0107, N., Kova\u010d, J., Kretschmer, N., Kladar, N., Smole Mo\u017eina, S., Bucar, F., Bauer, R., and Bo\u017ein, B. (2015). Natural products as antibacterial agents\u2014Antibacterial potential and safety of postdistillation and waste material from Thymus vulgaris L., Lamiaceae. Concepts, Compounds and the Alternatives of Antibacterials, InTech.","DOI":"10.5772\/60869"},{"key":"ref_55","first-page":"248","article-title":"Antimicrobial and resistance modulatory activity of Alpinia katsumadai seed phenolic extract, essential oil and post-distillation extract","volume":"52","author":"Bucar","year":"2014","journal-title":"Food Techn. Biotechnol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.mimet.2010.02.004","article-title":"Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts","volume":"81","author":"Piskernik","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"11733","DOI":"10.3390\/molecules181011733","article-title":"Impact of origin and biological source on chemical composition, anticholinesterase and antioxidant properties of some St. John\u2019 Wort species (Hypericum spp., Hypericaceae) from Central Balkans","volume":"18","author":"Kladar","year":"2013","journal-title":"Molecules"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.neubiorev.2005.03.009","article-title":"The tail suspension test as a model for assessing antidepressant activity: Review of pharmacological and genetic studies in mice","volume":"29","author":"Cryan","year":"2005","journal-title":"Neurosci. Biobehav. Rev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.ejphar.2005.12.014","article-title":"Identification of anxiolytic ingredients in ginseng root using elevated plus-maze test in mice","volume":"531","author":"Carr","year":"2006","journal-title":"Eur. J. Pharmacol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.pbb.2006.08.007","article-title":"Beneficial effects of galantamine on performance in the object recognition task in Swiss mice: Deficits induced by scopolamine and by prolonging the retention interval","volume":"85","author":"Pozet","year":"2006","journal-title":"Pharmacol. Biochem. Behav."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/18\/12\/1782\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T10:30:17Z","timestamp":1763980217000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/18\/12\/1782"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,24]]},"references-count":60,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ph18121782"],"URL":"https:\/\/doi.org\/10.3390\/ph18121782","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,24]]}}}