{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:55:25Z","timestamp":1764086125713,"version":"3.45.0"},"reference-count":63,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Junior Research Fellowship","award":["221610141586"],"award-info":[{"award-number":["221610141586"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Numerous degenerative diseases are brought on by inflammation and oxidative stress. Metabolites from plants contain anti-inflammatory and antioxidant properties. Indigenous and understudied, Thottea sivarajanii is a significant ethnobotanical herb. It is native to the Western Ghats and belongs to the Aristolochiaceae family. Objectives: The current study investigated the antioxidant and anti-inflammatory properties of T. sivarajanii leaf methanol extract (TSL) and the insights provided by phytochemical analysis. Methods: The HRLC\u2013MS\/MS (Q-TOF) study is used for the phytochemical analysis. The antioxidant efficacy is evaluated in terms of DPPH and ABTS radical scavenging, and reducing power (FRAP assay). In vitro anti-inflammatory efficacy was evaluated on RAW 264.7 cells challenged with lipopolysaccharide (LPS). Result: The HRLC\u2013MS\/MS (Q-TOF) study indicated the presence of bioactive molecules such as ursolic acid, Daidzein 4\u2019,7-diglucoside, Calophyllin B, and Berbamine, etc. The results showed in vitro antioxidant capacity in DPPH, and ABTS, radical scavenging, and ferric-reducing activities with respective IC50 and EC50 values of 184.5 \u00b1 2.4, 24.15 \u00b1 0.13, and 4.94 \u00b1 0.32 \u00b5g\/mL, respectively. LPS significantly stimulated the production of IL-1\u03b2, IL-6, and TNF-\u03b1 in RAW 264.7 cells (p &lt; 0.001). Treatment with TSL reduced levels of IL-1\u03b2 and IL-6 from 776.1 \u00b1 11.4 and 1678.1 \u00b1 12.4 to 195.4 \u00b1 9.2 and 465.4 \u00b1 11.8 pg\/mg protein. It also reduced NO levels from 91.4 \u00b1 1.3 to 30.8 \u00b1 1.7 \u00b5M\/mg protein while reducing TNF-\u03b1 levels from 2041.2 \u00b1 15.1 to 1037.5 \u00b1 15.4 pg\/mg protein. Conclusions: This work contributes to the growing evidence supporting the pharmacological importance of the underexplored Thottea sivarajanii, highlighting this species as a promising candidate for natural antioxidant and anti-inflammatory agents.<\/jats:p>","DOI":"10.3390\/ph18121794","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:29:47Z","timestamp":1764084587000},"page":"1794","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Screening by HRLC\u2013MS\/MS (Q-TOF) and Antioxidant and Anti-Inflammatory Properties of Thottea sivarajanii Leaf Extract"],"prefix":"10.3390","volume":"18","author":[{"given":"Pooja Mohan","family":"Padmalayam","sequence":"first","affiliation":[{"name":"Division of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph\u2019s College (Autonomous), Devagiri, Affiliated to University of Calicut, Calicut 673008, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aswathi Moothakoottil","family":"Kuttithodi","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph\u2019s College (Autonomous), Devagiri, Affiliated to University of Calicut, Calicut 673008, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3911-7540","authenticated-orcid":false,"given":"Alby","family":"Tom","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph\u2019s College (Autonomous), Devagiri, Affiliated to University of Calicut, Calicut 673008, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6693-1965","authenticated-orcid":false,"given":"Joice Tom","family":"Job","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph\u2019s College (Autonomous), Devagiri, Affiliated to University of Calicut, Calicut 673008, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satheesh","family":"George","sequence":"additional","affiliation":[{"name":"Department of Botany, St. Joseph\u2019s College (Autonomous), Devagiri, Affiliated to University of Calicut, Calicut 673008, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6232-1665","authenticated-orcid":false,"given":"Arunaksharan","family":"Narayanankutty","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, PG and Research Department of Zoology, St. Joseph\u2019s College (Autonomous), Devagiri, Affiliated to University of Calicut, Calicut 673008, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jomova, K., Alomar, S.Y., Valko, R., Liska, J., Nepovimova, E., Kuca, K., and Valko, M. 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