{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:38:43Z","timestamp":1791409123738,"version":"4.3.3"},"reference-count":84,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005916","name":"Vicerrector\u00eda de Investigaci\u00f3n, y Postgrado (VRIP) of Universidad de La Frontera","doi-asserted-by":"publisher","award":["TD23-0016"],"award-info":[{"award-number":["TD23-0016"]}],"id":[{"id":"10.13039\/501100005916","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"National Agency of Research and Development (ANID-Chile) through FONDECYT","doi-asserted-by":"publisher","award":["124-0141"],"award-info":[{"award-number":["124-0141"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"National Agency of Research and Development (ANID-Chile) through FONDECYT","doi-asserted-by":"publisher","award":["124-0688"],"award-info":[{"award-number":["124-0688"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007194","name":"DICYT","doi-asserted-by":"publisher","award":["022401RP"],"award-info":[{"award-number":["022401RP"]}],"id":[{"id":"10.13039\/100007194","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"ANID Ph.D. fellowship support","doi-asserted-by":"publisher","award":["21240481"],"award-info":[{"award-number":["21240481"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"ANID Ph.D. fellowship support","doi-asserted-by":"publisher","award":["21241628"],"award-info":[{"award-number":["21241628"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objective: Serotonin and dopamine are key neurotransmitters involved in regulating mood, anxiety, and locomotor activity. Specific transporters mediate their reuptake, SERT and DAT, making them targets for drugs such as Fluoxetine and Methylphenidate. Zebrafish (Danio rerio), due to their genetic and neurochemical similarity to humans, serve as a valuable model for studying the behavioral effects of these drugs. This study aimed to compare the behavioral phenotypes induced by SERT and DAT blockers in adult zebrafish using the Novel Tank Diving Test (NTT), thereby generating a swimming profile for drugs acting on these monoamine transporters that can be utilized in drug discovery and behavior. Methods: Adult zebrafish were administered Fluoxetine or Methylphenidate and subjected to the NTT. Behavioral endpoints measured included bottom-dwelling time (anxiety-like behavior), swimming velocity (locomotor activity), and transitions to the upper zone (exploratory behavior). Results: Fluoxetine treatment significantly reduced bottom-dwelling behavior, increased transitions to the upper zone, and decreased erratic swimming, indicating reduced anxiety and enhanced exploration. In contrast, Methylphenidate administration led to prolonged bottom-dwelling and reduced exploration, suggesting increased anxiety-like behavior and decreased exploration. These findings highlight distinct behavioral profiles resulting from selective modulation of serotonergic and dopaminergic pathways. Conclusions: The study demonstrates that SERT and DAT blockades produce divergent behavioral effects in adult zebrafish, with Fluoxetine exhibiting anxiolytic and exploratory-promoting actions. At the same time, Methylphenidate induces anxiety-like and less exploratory behaviors. These results underscore the utility of zebrafish as a valuable translational model for neuropharmacological research and drug discovery, providing insights into the differential impact of serotonergic and dopaminergic modulation on behavior.<\/jats:p>","DOI":"10.3390\/ph18121807","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T10:25:16Z","timestamp":1764239116000},"page":"1807","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Distinct Swimming Behavioral Phenotypes Following Serotonin and Dopamine Transporter Modulation in the Adult Zebrafish Novel Tank Diving Test (NTT)"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5468-4936","authenticated-orcid":false,"given":"Amaury","family":"Far\u00edas-Cea","sequence":"first","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda Molecular y Qu\u00edmica Medicinal, Departamento de Ciencias Qu\u00edmica y Recursos Naturales, Facultad de Ingenier\u00eda y Ciencias, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Laboratorio de Bioqu\u00edmica y Farmacolog\u00eda Molecular, Escuela de Ciencias, Facultad de Ciencias de la Vida, Universidad Vi\u00f1a del Mar, Vi\u00f1a del Mar 2572007, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisandra","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda Molecular y Qu\u00edmica Medicinal, Departamento de Ciencias Qu\u00edmica y Recursos Naturales, Facultad de Ingenier\u00eda y Ciencias, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de la Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crist\u00f3bal","family":"Leal","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda Molecular y Qu\u00edmica Medicinal, Departamento de Ciencias Qu\u00edmica y Recursos Naturales, Facultad de Ingenier\u00eda y Ciencias, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Programa de Doctorado en Ciencias Menci\u00f3n Biolog\u00eda Celular y Molecular Aplicada, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerim","family":"Segura","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda Molecular y Qu\u00edmica Medicinal, Departamento de Ciencias Qu\u00edmica y Recursos Naturales, Facultad de Ingenier\u00eda y Ciencias, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda Molecular y Qu\u00edmica Medicinal, Departamento de Ciencias Qu\u00edmica y Recursos Naturales, Facultad de Ingenier\u00eda y Ciencias, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caridad","family":"Ati\u00e9s-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda Molecular y Qu\u00edmica Medicinal, Departamento de Ciencias Qu\u00edmica y Recursos Naturales, Facultad de Ingenier\u00eda y Ciencias, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Programa de Doctorado en Ciencias Menci\u00f3n Biolog\u00eda Celular y Molecular Aplicada, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Miguel","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda Molecular y Qu\u00edmica Medicinal, Departamento de Ciencias Qu\u00edmica y Recursos Naturales, Facultad de Ingenier\u00eda y Ciencias, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Programa de Doctorado en Ciencias Menci\u00f3n Biolog\u00eda Celular y Molecular Aplicada, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4266-5647","authenticated-orcid":false,"given":"Martin","family":"H\u00f6dar-Salazar","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda Molecular y Qu\u00edmica Medicinal, Departamento de Ciencias Qu\u00edmica y Recursos Naturales, Facultad de Ingenier\u00eda y Ciencias, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Departamento de Procesos Diagn\u00f3sticos y Evaluaci\u00f3n, Facultad de Ciencias de la Salud, Universidad Cat\u00f3lica de Temuco, Temuco 4813302, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2376-990X","authenticated-orcid":false,"given":"Miguel","family":"Reyes-Parada","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica y Aplicada (CIBAP), Escuela de Medicina, Facultad de Ciencias M\u00e9dicas, Universidad de Santiago de Chile, Estaci\u00f3n Central 9170022, Chile"},{"name":"Facultad de Ciencias de la Salud, Universidad Aut\u00f3noma de Chile, Santiago 7500912, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1239-5367","authenticated-orcid":false,"given":"Ram\u00f3n","family":"Sotomayor-Z\u00e1rate","sequence":"additional","affiliation":[{"name":"Centro de Neurobiolog\u00eda y Fisiopatolog\u00eda Integrativa (CENFI), Instituto de Fisiolog\u00eda, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2360102, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Rojas-Hidalgo","sequence":"additional","affiliation":[{"name":"Grupo NeuroGastroBioqu\u00edmica, Instituto de Qu\u00edmica, Facultad de Ciencias, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Julio-Pieper","sequence":"additional","affiliation":[{"name":"Grupo NeuroGastroBioqu\u00edmica, Instituto de Qu\u00edmica, Facultad de Ciencias, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8084-4400","authenticated-orcid":false,"given":"Javier A.","family":"Bravo","sequence":"additional","affiliation":[{"name":"Grupo NeuroGastroBioqu\u00edmica, Instituto de Qu\u00edmica, Facultad de Ciencias, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5736-373X","authenticated-orcid":false,"given":"Dasiel O.","family":"Borroto-Escuela","sequence":"additional","affiliation":[{"name":"Receptomics and Brain Disorders Lab, IBIMA Plataforma 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