{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T02:35:25Z","timestamp":1766716525855,"version":"3.48.0"},"reference-count":51,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Ireland\u2014Taighde \u00c9ireann Government of Ireland Postgraduate Scholarship Programm","award":["GOIPG\/2023\/2741"],"award-info":[{"award-number":["GOIPG\/2023\/2741"]}]},{"name":"Research Ireland\u2014Taighde \u00c9ireann Government of Ireland Postgraduate Scholarship Programm","award":["GOIPG\/2021\/1434"],"award-info":[{"award-number":["GOIPG\/2021\/1434"]}]},{"name":"Research Ireland\u2014Taighde \u00c9ireann Government of Ireland Postgraduate Scholarship Programm","award":["GOIPG\/2020\/1496"],"award-info":[{"award-number":["GOIPG\/2020\/1496"]}]},{"name":"B. Braun Hospicare and Research Ireland\u2014Taighde \u00c9ireann","award":["13\/RC\/2073-P2"],"award-info":[{"award-number":["13\/RC\/2073-P2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Wound-related pain is a common, yet inadequately managed condition, and new therapeutic strategies are warranted. Limited data suggests that phytocannabinoids and cannabis may alleviate wound-related pain; however, further studies are required. This study investigated the effects of systemic administration of cannabidiol (CBD) on nociceptive behaviour following dorsum incision and on the endocannabinoid system. Methods: Male Sprague-Dawley rats (150\u2013200 g on arrival, n = 9\/group) underwent a 1.2 cm incision on the hairy skin of the dorsum or sham procedure. Back and hind paw mechanical withdrawal thresholds were assessed at baseline and post-surgery\/sham days (PSDs) 1, 4, 7, and 8 using manual and electronic von Frey tests, respectively. On PSD 8, the effect of a single acute administration of CBD (3, 10, or 30 mg\/kg, i.p.) on mechanical hypersensitivity in the dorsum and hind paws was assessed. The levels of endocannabinoids and N-acylethanolamines in the plasma and discrete brain regions following CBD administration were analysed. Results: Robust mechanical hypersensitivity was evident in the dorsum and hind paws following the incision. CBD (3 mg\/kg) partially attenuated primary mechanical hypersensitivity in the dorsum, in a site- and dose-specific manner. CBD had no effect on secondary mechanical hypersensitivity. CBD did not alter the levels of endocannabinoids or N-acylethanolamines, but in rats that received CBD (3 mg\/kg), levels of 2-AG were lower in the contralateral amygdala and levels of AEA were higher in the contralateral lumbar spinal cord, compared to the ipsilateral sides. Conclusions: These data provide evidence for antinociceptive effects of CBD in a model of incisional wound-related pain. Further research on CBD\u2019s mechanism(s) of action is warranted. The potential antinociceptive effects of other phytocannabinoids in this model should also be investigated.<\/jats:p>","DOI":"10.3390\/ph19010043","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T14:27:51Z","timestamp":1766586471000},"page":"43","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Effect of Cannabidiol on Nociceptive Behaviour and the Endocannabinoid System in an Incisional Wound Model"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9499-5499","authenticated-orcid":false,"given":"Maria C.","family":"Redmond","sequence":"first","affiliation":[{"name":"Pharmacology and Therapeutics, School of Pharmacy and Medical Sciences, Institute for Health Discovery and Innovation, University of Galway, H91 W5P7 Galway City, Ireland"},{"name":"Galway Neuroscience Centre, University of Galway, H91 TK33 Galway City, Ireland"},{"name":"Centre for Pain Research, University of Galway, H91 TK33 Galway City, Ireland"},{"name":"C\u00daRAM, Research Ireland Centre for Medical Devices, University of Galway, H91 W2TY Galway City, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1072-6801","authenticated-orcid":false,"given":"Catherine R.","family":"Healy","sequence":"additional","affiliation":[{"name":"Pharmacology and Therapeutics, School of Pharmacy and Medical Sciences, Institute for Health Discovery and Innovation, University of Galway, H91 W5P7 Galway City, Ireland"},{"name":"Galway Neuroscience Centre, University of Galway, H91 TK33 Galway City, Ireland"},{"name":"Centre for Pain Research, University of Galway, H91 TK33 Galway City, Ireland"},{"name":"C\u00daRAM, Research Ireland Centre for Medical Devices, University of Galway, H91 W2TY Galway City, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9797-3850","authenticated-orcid":false,"given":"Mary","family":"Hopkins","sequence":"additional","affiliation":[{"name":"Pharmacology and Therapeutics, School of Pharmacy and Medical Sciences, Institute for Health Discovery and Innovation, University of Galway, H91 W5P7 Galway City, Ireland"},{"name":"Galway Neuroscience Centre, University of Galway, H91 TK33 Galway City, Ireland"},{"name":"Centre for Pain Research, University of Galway, H91 TK33 Galway City, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1266-6881","authenticated-orcid":false,"given":"Rosmara","family":"Infantino","sequence":"additional","affiliation":[{"name":"Pharmacology and Therapeutics, School of Pharmacy and Medical Sciences, Institute for Health Discovery and Innovation, University of Galway, H91 W5P7 Galway City, Ireland"},{"name":"Galway Neuroscience Centre, University of Galway, H91 TK33 Galway City, Ireland"},{"name":"Centre for Pain Research, University of Galway, H91 TK33 Galway City, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgina","family":"Gethin","sequence":"additional","affiliation":[{"name":"C\u00daRAM, Research Ireland Centre for Medical Devices, University of Galway, H91 W2TY Galway City, Ireland"},{"name":"School of Nursing and Midwifery, University of Galway, H91 E3YV Galway City, Ireland"},{"name":"Alliance for Research and Innovation in Wounds, University of Galway, H91 TK33 Galway City, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6292-4933","authenticated-orcid":false,"given":"Abhay","family":"Pandit","sequence":"additional","affiliation":[{"name":"C\u00daRAM, Research Ireland Centre for Medical Devices, University of Galway, H91 W2TY Galway City, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6186-621X","authenticated-orcid":false,"given":"David P.","family":"Finn","sequence":"additional","affiliation":[{"name":"Pharmacology and Therapeutics, School of Pharmacy and Medical Sciences, Institute for Health Discovery and Innovation, University of Galway, H91 W5P7 Galway City, Ireland"},{"name":"Galway Neuroscience Centre, University of Galway, H91 TK33 Galway City, Ireland"},{"name":"Centre for Pain Research, University of Galway, H91 TK33 Galway City, Ireland"},{"name":"C\u00daRAM, Research Ireland Centre for Medical Devices, University of Galway, H91 W2TY Galway City, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e1075","DOI":"10.1097\/PR9.0000000000001075","article-title":"Prevalence of postoperative pain after hospital 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