{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T18:26:18Z","timestamp":1769538378318,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T00:00:00Z","timestamp":1769299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Science and Technology Development Fund, Macau S.A.R","award":["0029\/2021\/ITP"],"award-info":[{"award-number":["0029\/2021\/ITP"]}]},{"name":"The Science and Technology Development Fund, Macau S.A.R","award":["0073\/2023\/RIA2"],"award-info":[{"award-number":["0073\/2023\/RIA2"]}]},{"name":"The Science and Technology Development Fund, Macau S.A.R","award":["0002\/2025\/NRP"],"award-info":[{"award-number":["0002\/2025\/NRP"]}]},{"DOI":"10.13039\/501100004733","name":"Research Fund of University of Macau","doi-asserted-by":"publisher","award":["MYRG-GRG2023-00048-ICMS"],"award-info":[{"award-number":["MYRG-GRG2023-00048-ICMS"]}],"id":[{"id":"10.13039\/501100004733","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82204482"],"award-info":[{"award-number":["82204482"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2024A1515010167"],"award-info":[{"award-number":["2024A1515010167"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Laboratory of Sichuan Province for Traditional Chinese Medicine Regimen and Health","award":["GZ2022003"],"award-info":[{"award-number":["GZ2022003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: The rhizome of Gastrodia elata Blume (Tianma) is a functional food with medicinal value in China, used to improve the health of the central nervous system and reported to exhibit anti-cellular senescent activity. P-hydroxybenzaldehyde (P-HBA) is a key aromatic compound isolated from Tianma; however, its potential to mitigate cellular senescence remains unclear. Methods: We employed ultra-performance liquid chromatography-mass spectrometry to identify the chemical characterization of Tianma extract. Cell viability assay, senescence-associated-\u03b2-galactosidase (SA-\u03b2-Gal) assay, and immunofluorescence staining and autophagy analysis were used to evaluate the anti-senescent activity of P-HBA and other Tianma components. Results: Our findings demonstrate that Tianma methanol extract (TME) and P-HBA significantly reduce cellular senescent inducer hydroxyurea (HU)-induced DNA damage, SA-\u03b2-Gal activity increase, and autophagic dysfunction in human SH-SY5Y cells. Notably, an autophagy inhibitor, chloroquine, can reduce anti-cellular senescent activity of P-HBA. Conclusions: These results suggest that P-HBA exhibits the effect of reducing cellular senescent phenotypes, and its effect is achieved by enhancing autophagy.<\/jats:p>","DOI":"10.3390\/ph19020207","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T10:24:36Z","timestamp":1769423076000},"page":"207","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["P-Hydroxybenzaldehyde from Gastrodia elata Blume Reduces Hydroxyurea-Induced Cellular Senescent Phenotypes in Human SH-SY5Y Cells via Enhancing Autophagy"],"prefix":"10.3390","volume":"19","author":[{"given":"Shuhui","family":"Qu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daijiao","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingxuan","family":"Fan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Dai","sequence":"additional","affiliation":[{"name":"School of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1247-3150","authenticated-orcid":false,"given":"Hai-Jing","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioactive Molecules and Druggability Assessment, Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs, College of Pharmacy, Jinan University, Guangzhou 510632, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8849-0280","authenticated-orcid":false,"given":"Wei","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheong-Meng","family":"Chong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lu, C., Qu, S., Zhong, Z., Luo, H., Lei, S.S., Zhong, H.J., Su, H., Wang, Y., and Chong, C.M. 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