{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T19:23:11Z","timestamp":1770319391178,"version":"3.49.0"},"reference-count":27,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MTA-SE \u201cLend\u00fclet\u201d","award":["LP2021-3\/2021"],"award-info":[{"award-number":["LP2021-3\/2021"]}]},{"DOI":"10.13039\/501100012550","name":"NRDI Fund","doi-asserted-by":"crossref","award":["STAGE 2024-1.2.3-HU-RIZONT-2024-00056"],"award-info":[{"award-number":["STAGE 2024-1.2.3-HU-RIZONT-2024-00056"]}],"id":[{"id":"10.13039\/501100012550","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012550","name":"National Research, Development and Innovation Fund","doi-asserted-by":"crossref","award":["TKP2021-EGA"],"award-info":[{"award-number":["TKP2021-EGA"]}],"id":[{"id":"10.13039\/501100012550","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University of Debrecen for Open Access Publications"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Fluvoxamine (FLU) is a selective serotonin reuptake inhibitor and one of the most potent agonists of the sigma-1 receptor. Emerging evidence shows that FLU exerts protective effects in multiple organs, making it a promising candidate for topical ocular therapy. Developing an FLU eyedrop for glaucoma can address a significant treatment gap with potentially fewer side effects compared with conventional therapies. To optimise formulation development, precise quantification of FLU in ocular compartments such as aqueous humour, as well as systemic circulation, is essential to characterise drug absorption, ocular bioavailability, and safety. Methods: We developed and validated a UHPLC-MS method for FLU detection in aqueous humour and serum using simple sample preparation steps. Results: The 11-min-long reverse phase chromatography followed by SRM-based mass spectrometry detection provides a highly selective and sensitive FLU detection method. Our method was proved to be linear in the 0.0625\u20131.5 \u00b5g\/mL range and was validated according to the EMA guidelines. Conclusions: The simplicity of sample preparation, the tolerable matrix effects, and the favourable detection parameters provide a robust tool for preclinical pharmacokinetic and pharmacodynamic studies of FLU\u2019s ocular protective effects.<\/jats:p>","DOI":"10.3390\/ph19020260","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T09:00:58Z","timestamp":1770109258000},"page":"260","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UHPLC-MS-Based Analysis of Fluvoxamine in Rabbit Aqueous Humour and Serum: Method Development and Validation"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0535-2025","authenticated-orcid":false,"given":"Andrea","family":"Guba","sequence":"first","affiliation":[{"name":"Proteomics Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Egyetem t\u00e9r 1, 4032 Debrecen, Hungary"},{"name":"Metabolomics Research Group, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Egyetem t\u00e9r 1, 4032 Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4756-9136","authenticated-orcid":false,"given":"Anna","family":"Tak\u00e1csi-Nagy","sequence":"additional","affiliation":[{"name":"Department of Innovation, PannonPharma Ltd., PannonPharma \u00fat 1., 7720 P\u00e9csv\u00e1rad, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3753-9689","authenticated-orcid":false,"given":"Sourav","family":"Das","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs Medical School, Vasv\u00e1ri P\u00e1l utca 4, 7622 P\u00e9cs, Hungary"},{"name":"J\u00e1nos Szent\u00e1gothai Research Centre, University of P\u00e9cs Medical School, Ifj\u00fas\u00e1g \u00fatja 20, 7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0196-6072","authenticated-orcid":false,"given":"B\u00e1lint","family":"Szokol","sequence":"additional","affiliation":[{"name":"Vichem Chemie Research Ltd., Viola utca 2., 8200 Veszpr\u00e9m, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3618-6690","authenticated-orcid":false,"given":"Medveczki","family":"Timea","sequence":"additional","affiliation":[{"name":"MTA-SE Lend\u00fclet \u201cMomentum\u201d Diabetes Research Group, Semmelweis University, B\u00f3kay J\u00e1nos utca 54, 1083 Budapest, Hungary"},{"name":"Pediatric Center, MTA Center of Excellence, Semmelweis University, B\u00f3kay J\u00e1nos utca 54, 1083 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rton","family":"Vajna","sequence":"additional","affiliation":[{"name":"University Pharmacy-Department of Pharmacy Administration, Semmelweis University, H\u0151gyes Endre utca 7-9, 1092 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5732-8758","authenticated-orcid":false,"given":"Gerg\u0151","family":"Kall\u00f3","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Egyetem t\u00e9r 1, 4032 Debrecen, Hungary"},{"name":"Metabolomics Research Group, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Egyetem t\u00e9r 1, 4032 Debrecen, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Fekete","sequence":"additional","affiliation":[{"name":"MTA-SE Lend\u00fclet \u201cMomentum\u201d Diabetes Research Group, Semmelweis University, B\u00f3kay J\u00e1nos utca 54, 1083 Budapest, Hungary"},{"name":"Pediatric Center, MTA Center of Excellence, Semmelweis University, B\u00f3kay J\u00e1nos utca 54, 1083 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6388-4021","authenticated-orcid":false,"given":"Judit","family":"Hodrea","sequence":"additional","affiliation":[{"name":"MTA-SE Lend\u00fclet \u201cMomentum\u201d Diabetes Research Group, Semmelweis University, B\u00f3kay J\u00e1nos utca 54, 1083 Budapest, Hungary"},{"name":"Pediatric Center, MTA Center of Excellence, Semmelweis University, B\u00f3kay J\u00e1nos utca 54, 1083 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4373-2175","authenticated-orcid":false,"given":"\u00c9va","family":"Cs\u0151sz","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Egyetem t\u00e9r 1, 4032 Debrecen, 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