{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:52:21Z","timestamp":1790823141782,"version":"4.1.0"},"reference-count":35,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of \u201cInter-Governmental Cooperation in Science and Technology Innovation\u201d","award":["2023YFE0103200"],"award-info":[{"award-number":["2023YFE0103200"]}]},{"name":"Shenzhen Science and Technology Plan Project","award":["KXXFZ20211020172545006"],"award-info":[{"award-number":["KXXFZ20211020172545006"]}]},{"DOI":"10.13039\/501100017607","name":"Shenzhen Basic Research Project","doi-asserted-by":"publisher","award":["JCYJ20240813160804007"],"award-info":[{"award-number":["JCYJ20240813160804007"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Influenza A virus is an acute respiratory virus that spreads quickly, affects a broad range of populations, and can lead to many complications and mortality. Artemisia L. species are widely used in traditional medicine, but their antiviral potential against H1N1 remains uncertain. Methodology: Network pharmacology and molecular docking were used to computationally explore their potential function in this domain, and to investigate how their invasion mechanisms and adsorption occur. UPLC-MS\/MS analysis identified the main components of the extracts. The anti-H1N1 mechanism of Artemisia L. extracts was studied in vitro. Results: Network pharmacology identified 95 key targets between Artemisia L. and IAV, with quercetin and luteolin as core active compounds. Molecular docking predicted strong binding affinities between these compounds and influenza virus proteins. UPLC-MS\/MS analysis identified 75, 100, and 64 chemical components in ACBE, AALE, and ACTE, respectively, mainly flavonoids and terpenoids. Artemisia L. extracts exhibited both preventive and therapeutic effects against H1N1, reducing progeny virus NP mRNA and protein levels. In vitro experiments showed that higher concentrations of the extracts prevent virus attachment to MDCK cells by denaturing the HA protein. NA plays an essential role in progeny virus release. We found that a high concentration of ACTE can inhibit NA up to 85%, and ACBE showed a low inhibitory effect on NA. Conclusions: In terms of therapeutic effects, Artemisia L. extracts can regulate intracellular inflammatory factors via the TLR4\/NF-\u03baB\/MyD88 signaling pathways and reduce the expression of IL-1\u03b2, IL-6, TNF-\u03b1, TLR4, NF-\u03baB, p65, and MyD88 at the mRNA level, thereby inhibiting H1N1 virus replication. These results suggest that bioactive components in Artemisia L. extracts may inhibit H1N1, potentially leading to the development of natural-product-based anti-influenza agents.<\/jats:p>","DOI":"10.3390\/ph19020275","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T16:32:59Z","timestamp":1770395579000},"page":"275","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Artemisia Extracts Suppress H1N1 Influenza A Virus Infection by Targeting Viral HA\/NA Proteins and Modulating the TLR4\/MyD88\/NF-\u03baB Signaling Axis"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4565-3961","authenticated-orcid":false,"given":"Zhongnan","family":"Hu","sequence":"first","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"},{"name":"Zhejiang International Joint Laboratory of Traditional Medicine and Big Health Products Development, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Wu","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tayyab","family":"Ali","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"},{"name":"Zhejiang International Joint Laboratory of Traditional Medicine and Big Health Products Development, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7559-8903","authenticated-orcid":false,"given":"Adam","family":"Junka","sequence":"additional","affiliation":[{"name":"\u201cPUMA\u201d, Platform for Unique Model Applications, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-534 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0378-8887","authenticated-orcid":false,"given":"Farukh S.","family":"Sharopov","sequence":"additional","affiliation":[{"name":"Research Institution \u201cChinese-Tajik Innovation Center for Natural Products\u201d, National Academy of Sciences of Tajikistan, Dushanbe 734063, Tajikistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zou","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shisong","family":"Fang","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"},{"name":"Zhejiang International Joint Laboratory of Traditional Medicine and Big Health Products Development, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00040-00022","DOI":"10.1128\/cmr.00040-22","article-title":"Antiviral Approaches against Influenza Virus","volume":"36","author":"Kumari","year":"2023","journal-title":"Clin. 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