{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T06:17:50Z","timestamp":1770877070446,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Ulcerative colitis (UC) is a chronic inflammatory disease marked by mucosal injury and immune dysregulation. Gegen Qinlian Decoction (GQD) shows therapeutic potential, but extraction-dependent reproducibility remains unclear. Methods: We systematically searched PubMed, Scopus, and Web of Science for studies evaluating GQD aqueous decoctions or ethanolic extracts in DSS-induced colitis. Main outcome: Disease Activity Index (DAI). Key additional outcomes: colon length and histological injury; cytokines and microbiota were also assessed. Random-effects models with Hartung\u2013Knapp\u2013Sidik\u2013Jonkman adjustment, subgroup analyses, and exploratory dose\u2013response meta-regression were applied. Results: Eight studies were included (aqueous: 5; ethanolic: 3; 209 mice). GQD significantly improved DAI (SMD \u22122.17; p &lt; 0.00001; I2 = 43%), colon length (MD 1.18 cm; p &lt; 0.00001; I2 = 88%), and histological injury (SMD \u22123.02; p &lt; 0.0001; I2 = 51%). For DAI, both preparations favored GQD, with absent heterogeneity in aqueous studies (I2 = 0%) vs. substantial variability in ethanolic extracts (I2 = 75%). For histology, subgroup differences suggested a larger effect size with ethanolic extracts, with higher heterogeneity (I2 = 60% vs. 0%). In the aqueous subset, GQD reduced inflammatory markers and increased microbial diversity. Dose\u2013response meta-regression was performed within the aqueous subset as an exploratory analysis. Conclusions: Aqueous decoctions showed the most reproducible profile across key endpoints, whereas ethanolic extracts were more variable despite a larger histology point estimate, indicating that the extraction matrix meaningfully influences translational consistency in preclinical research.<\/jats:p>","DOI":"10.3390\/ph19020277","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T16:32:59Z","timestamp":1770395579000},"page":"277","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extraction Matrix Shapes the Efficacy of Gegen Qinlian Decoction in DSS-Induced Colitis: A Preclinical Systematic Review and Meta-Analysis"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2355-2217","authenticated-orcid":false,"given":"Carlos R.","family":"Montes-de-Oca-Saucedo","sequence":"first","affiliation":[{"name":"Department of Histology, Faculty of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Brice\u00f1o-Villardaga","sequence":"additional","affiliation":[{"name":"Department of Histology, Faculty of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n R.","family":"Fuentes-Salinas","sequence":"additional","affiliation":[{"name":"Department of Histology, Faculty of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolfo","family":"Soto-Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Department of Histology, Faculty of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), Monterrey 64460, Nuevo Le\u00f3n, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/j.dld.2021.03.002","article-title":"Quality of life in patients with moderate to severe ulcerative colitis and the impact of treatment: A narrative review","volume":"53","author":"Armuzzi","year":"2021","journal-title":"Dig. 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