{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:18:17Z","timestamp":1790720297726,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EraNet Neuron grant BiotaBB","award":["32NE30_213470"],"award-info":[{"award-number":["32NE30_213470"]}]},{"name":"FNS grant","award":["32NE30_213470"],"award-info":[{"award-number":["32NE30_213470"]}]},{"name":"Ram\u00f3n Areces Foundation post-doctoral fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Stroke is a major global risk to human health due to its high incidence, mortality, and prevalence of associated long-term disabilities. Recent studies have highlighted a significant impact of the gut\u2013brain axis and metabolites derived from intestinal microbiota on modulating neurological disorders, including stroke. Methods: In this study, we investigated the effects of pre- and post-treatment with D-lactate, a lactate stereoisomer mainly produced by certain gut bacteria, on stroke outcome using a transient middle cerebral artery occlusion (MCAO) mouse model. For this purpose, male C57BL\/6J mice received a single administration of D-lactate or vehicle (PBS) via the tail vein either before the MCAO surgery, as a preventive approach, or upon reperfusion, as a therapeutic paradigm. Functional outcome was assessed daily using a standard neuroscore and the adhesive removal test until day three post-surgery, when mice were sacrificed. Results: Our results indicated no significant difference in infarct size, measured using cresyl violet staining, between the D-lactate and PBS groups in both pre- and post-treatment experiments. In addition, evaluation of neurological deficits and sensorimotor function showed no statistically significant differences between the interventions throughout the experiment. Conclusions: The present data suggest that treatment with D-lactate does not show a beneficial effect in our C57BL\/6J mouse MCAO model.<\/jats:p>","DOI":"10.3390\/ph19030410","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T16:06:59Z","timestamp":1772467619000},"page":"410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploration of the Preventive and Therapeutic Effects of D-Lactate Administration in a Mouse MCAO Model"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8472-5137","authenticated-orcid":false,"given":"Seyedeh Maryam","family":"Mousavi","sequence":"first","affiliation":[{"name":"Stroke Laboratory, Neurology Service, Department of Clinical Neurosciences, Lausanne University Hospital Centre and University of Lausanne, CH-1011 Lausanne, Switzerland"},{"name":"Department of Fundamental Neurosciences, University of Lausanne, CH-1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8564-649X","authenticated-orcid":false,"given":"Lara","family":"Buscemi","sequence":"additional","affiliation":[{"name":"Stroke Laboratory, Neurology Service, Department of Clinical Neurosciences, Lausanne University Hospital Centre and University of Lausanne, CH-1011 Lausanne, Switzerland"},{"name":"Department of Fundamental Neurosciences, University of Lausanne, CH-1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Castillo-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Stroke Laboratory, Neurology Service, Department of Clinical Neurosciences, Lausanne University Hospital Centre and University of Lausanne, CH-1011 Lausanne, Switzerland"},{"name":"Department of Fundamental Neurosciences, University of Lausanne, CH-1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Price","sequence":"additional","affiliation":[{"name":"Stroke Laboratory, Neurology Service, Department of Clinical Neurosciences, Lausanne University Hospital Centre and University of Lausanne, CH-1011 Lausanne, Switzerland"},{"name":"Department of Fundamental Neurosciences, University of Lausanne, CH-1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2921-5000","authenticated-orcid":false,"given":"Lorenz","family":"Hirt","sequence":"additional","affiliation":[{"name":"Stroke Laboratory, Neurology Service, Department of Clinical Neurosciences, Lausanne University Hospital Centre and University of Lausanne, CH-1011 Lausanne, Switzerland"},{"name":"Department of Fundamental Neurosciences, University of Lausanne, CH-1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.ncl.2016.06.013","article-title":"Epidemiology of Ischemic Stroke","volume":"34","author":"Favate","year":"2016","journal-title":"Neurol. 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