{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T06:00:31Z","timestamp":1774159231206,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005667","name":"FAPESC","doi-asserted-by":"publisher","award":["2024TR000070"],"award-info":[{"award-number":["2024TR000070"]}],"id":[{"id":"10.13039\/501100005667","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CAPES Finance","award":["001\/PROAP\/PPG-BQA"],"award-info":[{"award-number":["001\/PROAP\/PPG-BQA"]}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["305891\/2023-1"],"award-info":[{"award-number":["305891\/2023-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycemia and insulin resistance, leading to progressive metabolic dysfunction. Flavonoids, such as astragalin, have reported antidiabetic potential; however, their direct effects on pancreatic \u03b2-cell ionic mechanisms and insulin secretion remain unclear. This study aimed to investigate the effects of astragalin on glucose uptake, insulin secretion, and membrane ionic currents in pancreatic \u03b2-cell lines. Methods: Murine MIN6 and rat INS-1 pancreatic \u03b2-cells were used as experimental models. Following astragalin treatment, glucose uptake was quantified by bioluminescence, and insulin secretion was measured by ELISA. Ionic currents were analyzed using the whole-cell patch-clamp technique. Selective pharmacological blockers targeting ATP-sensitive K+ channels (KATP), voltage-dependent K+ channels (Kv), and L-type voltage-dependent Ca2+ channels were applied to elucidate the underlying mechanisms. Results: Astragalin increased glucose uptake in a time-dependent manner, reaching a plateau between 3 and 5 h. Insulin secretion was significantly enhanced after 1 h of exposure to 100 \u00b5M astragalin. Patch-clamp recordings demonstrated that astragalin reduced potassium channel currents in pancreatic \u03b2-cells. Pharmacological modulation confirmed the involvement of KATP, Kv, and L-type Ca2+ channels. Verapamil attenuated the insulinotropic effect, supporting the role of calcium influx in astragalin-induced insulin exocytosis. Conclusions: Astragalin enhances glucose uptake and stimulates insulin secretion in pancreatic \u03b2-cells through modulation of potassium and calcium channels, promoting calcium-dependent exocytosis. These findings support its potential as a candidate for antidiabetic therapeutic strategies.<\/jats:p>","DOI":"10.3390\/ph19030508","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T10:49:58Z","timestamp":1774003798000},"page":"508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["How Astragalin Modulates Glucose Uptake and Insulin Secretion in \u03b2-Cell Lines"],"prefix":"10.3390","volume":"19","author":[{"given":"Paola Miranda","family":"Sulis","sequence":"first","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice Lima Rosa","family":"Mendes","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula Waiss Zanusso","family":"Bunick","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8932-919X","authenticated-orcid":false,"given":"Karina","family":"Cesca","sequence":"additional","affiliation":[{"name":"Departamento de Engenharia Qu\u00edmica e de Alimentos, Centro de Tecnologia, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carine","family":"Royer","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"},{"name":"Laborat\u00f3rio de Farmacologia Molecular, Universidade de Bras\u00edlia, Bras\u00edlia 70900-910, Brazil"},{"name":"Faculdade de Ci\u00eancias e Tecnologias em Sa\u00fade, Universidade de Bras\u00edlia, Bras\u00edlia 70900-910, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna Antunes","family":"Zaniboni","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Carvalho","family":"Cavalari","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6920-0049","authenticated-orcid":false,"given":"Guilherme Brasil","family":"Pintarelli","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"},{"name":"Departamento de Engenharia de Controle, Automa\u00e7\u00e3o e Computa\u00e7\u00e3o, Universidade Federal de Santa Catarina, Blumenau 89065-200, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Luiz Andreotti","family":"Dagostin","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, Brazil"},{"name":"Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1tima Regina Mena Barreto","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto de Bioeletricidade Celular (IBIOCEL), Ci\u00eancia & Sa\u00fade, Departamento de Bioqu\u00edmica, Centro de Ci\u00eancia Biol\u00f3gicas, Universidade Federal de Santa Catarina, Florian\u00f3polis 88037-000, 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