{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:30:16Z","timestamp":1788402616863,"version":"build-2803163510"},"reference-count":41,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010828","name":"Department of Education of Guizhou Province","doi-asserted-by":"crossref","award":["[2023]015"],"award-info":[{"award-number":["[2023]015"]}],"id":[{"id":"10.13039\/501100010828","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Undergraduate Innovation and Entrepreneurship Training Program of China","award":["S202510660300"],"award-info":[{"award-number":["S202510660300"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: The absence of disease-modifying treatments for Parkinson\u2019s disease (PD)\u2014a neurodegenerative condition with escalating global incidence\u2014represents a critical unmet medical need. Traditionally utilized for both dietary consumption and medicinal preparations, the fruit derived from Rosa roxburghii Tratt demonstrates a remarkably rich profile of biologically active compounds, with flavonoids, triterpenoids, and organic acids representing the predominant classes. Experimental evidence indicates that these compounds elicit robust antioxidative, anti-inflammatory, and neuroprotective effects, making them promising candidates for neurodegenerative disease modulation. This study aimed to systematically evaluate the neuroprotective effects of Rosa roxburghii Tratt juice concentrate powder (RRJCP) across the preventive, interventional, and therapeutic stages of PD and to elucidate its underlying molecular mechanisms. Methods: Rosa roxburghii Tratt juice was subjected to rotary evaporation concentration and vacuum freeze-drying to obtain the juice concentrate powder. C57BL\/6 mice were randomly assigned to three main groups (prevention, intervention, and treatment), each containing subgroups including a normal control, an MPTP model group, low-, medium-, and high-dose RRCJP groups (50, 100, and 200 mg\/kg), and a positive control Madopar group, totaling 18 subgroups. A chronic MPTP-induced PD mouse model was established. Motor function was assessed via the open field test, pole test, and wire hang test. Substantia nigra neuronal morphology was examined by hematoxylin and eosin staining. The area of tyrosine hydroxylase (TH)-positive regions was measured by immunohistochemistry. The levels of oxidative stress indicators in serum were measured using biochemical kits. Network pharmacology was employed to predict core targets, and the expression of PI3K\/AKT pathway and apoptosis-related proteins was determined by Western blotting. Results: Compared with the MPTP model group, RRCJP (200 mg\/kg) significantly increased the total distance traveled in the open field, shortened the pole climbing time, and improved the wire hang score. It attenuated the morphological disorganization and nuclear pyknosis of substantia nigra neurons, increased the TH-positive area and TH protein expression, reduced serum MDA content, and elevated the activities of SOD and GSH-Px. Network pharmacology analysis indicated that the PI3K\/AKT signaling pathway was among the core targets. Western blotting results further showed that the juice concentrate powder upregulated the expression of p-PI3K, p-AKT, and Bcl-2, while downregulating Bax and Cleaved Caspase-3 levels, which was consistent with the network pharmacology prediction. Conclusions: RRCJP exerts neuroprotective effects across the preventive, interventional, and therapeutic stages in PD model mice, the mechanisms of which may be associated with activation of the PI3K\/AKT signaling pathway, attenuation of oxidative stress, and inhibition of neuronal apoptosis.<\/jats:p>","DOI":"10.3390\/ph19050711","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:32:21Z","timestamp":1777555941000},"page":"711","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Neuroprotective Effects of Rosa roxburghii Tratt Juice Concentrate Powder in Parkinson\u2019s Disease Mice via the PI3K\/AKT Signaling Pathway"],"prefix":"10.3390","volume":"19","author":[{"given":"Tong","family":"Jin","sequence":"first","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faguang","family":"Kuang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjie","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haonan","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiapan","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yikai","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baofei","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"},{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Natural Products Research Center of Guizhou Province, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Human Brain Bank for Functions and Diseases, Department of Education of Guizhou Province, College of Basic Medical, Guizhou Medical University, Guiyang 561113, China"},{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Natural Products Research Center of Guizhou Province, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e464","DOI":"10.1016\/S2666-7568(24)00094-1","article-title":"Temporal trends in the prevalence of Parkinson\u2019s disease from 1980 to 2023: A systematic review and meta-analysis","volume":"5","author":"Zhu","year":"2024","journal-title":"Lancet Healthy Longev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1001\/jama.2019.22360","article-title":"Diagnosis and Treatment of Parkinson Disease: A Review","volume":"323","author":"Armstrong","year":"2020","journal-title":"JAMA"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Church, F.C. 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