{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:01:43Z","timestamp":1785427303662,"version":"3.56.0"},"reference-count":52,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015308","name":"Xinjiang Uygur Autonomous Region","doi-asserted-by":"publisher","award":["2023B03002-4"],"award-info":[{"award-number":["2023B03002-4"]}],"id":[{"id":"10.13039\/501100015308","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015308","name":"Xinjiang Uygur Autonomous Region","doi-asserted-by":"publisher","award":["TSYC202301B132"],"award-info":[{"award-number":["TSYC202301B132"]}],"id":[{"id":"10.13039\/501100015308","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2023B03002-4"],"award-info":[{"award-number":["2023B03002-4"]}],"id":[{"id":"https:\/\/ror.org\/02vtvez67","id-type":"ROR","asserted-by":"publisher"}]},{"award":["TSYC202301B132"],"award-info":[{"award-number":["TSYC202301B132"]}],"id":[{"id":"https:\/\/ror.org\/02vtvez67","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: WenTong HuoXue Cream (WTHXC) plays a significant role in the treatment of diabetic peripheral neuropathy (DPN). However, the material basis and quality control methods for this formulation remain largely unexplored. Methods: In this study, UPLC-HRMS\/MS combined with standard reference substances was employed to comprehensively identify and confirm the chemical constituents of WTHXC. Furthermore, a rapid and sensitive UPLC-MS\/MS method based on multiple reaction monitoring (MRM) mode was developed and validated for the simultaneous quantification of the marker components. Results: Nine compounds were unambiguous characterized, including Di-hydrocapsaicin (DHC), Oxypeucedanin hydrate (OPH), Imperatorin (IMP), Isoimperatorin (IIMP), Xanthotoxin (XAN), Hydroxysafflor yellow A (HSYA), Chlorogenic acid (CGA), Ferulic Acid (FA) and Ligustilide (LIG). The results of method validation denotes that all the analytes showed good linearity between concentration and peak area in the tested ranges, with correlation coefficients (r) not less than 0.9990. The relative standard deviation (RSD) of precision was in the range of 0.57\u20137.11%. The accuracy of the method, verified by recovery experiments at three concentration levels, ranged from 96.51% to 101.04% for all analytes. Transdermal behavior determination results demonstrate that OPH, HYSA, CGA, FA and LIG exhibited favorable skin permeability and may serve as the key active components of WTHXC. Conclusions: This study elucidates the material basis of WTHXC, providing a scientific foundation for the development of quality control methods and facilitating its broader clinical application.<\/jats:p>","DOI":"10.3390\/ph19060805","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T05:33:54Z","timestamp":1779687234000},"page":"805","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification, Determination and Transdermal Behavior Characterization of Nine Components in WenTong HuoXue Cream by UPLC-MS\/MS"],"prefix":"10.3390","volume":"19","author":[{"given":"Xinran","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Xinjiang Medical University, No. 567 Shangde North Road, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2480-4728","authenticated-orcid":false,"given":"Xiaodan","family":"Qiu","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Tian Tan Xi Li, Beijing 100050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Kang","sequence":"additional","affiliation":[{"name":"Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine, No. 116 Huanghe Road, Urumqi 830002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0646-2874","authenticated-orcid":false,"given":"Guangzhi","family":"Shan","sequence":"additional","affiliation":[{"name":"Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Tian Tan Xi Li, Beijing 100050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghui","family":"He","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Uyghur Medical Research, Xinjiang Institute of Materia Medica, Urumqi 830004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"ref_1","first-page":"1665","article-title":"Clinical observation on painful diabetic neuropathy treated by Wentong Huoxue Cream plus limb air-pressure therapy: A report of 30 cases","volume":"52","author":"Ma","year":"2011","journal-title":"J. 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