{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:02:51Z","timestamp":1791050571524,"version":"4.1.0"},"reference-count":62,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/01207\/2018"],"award-info":[{"award-number":["CEECIND\/01207\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/00102\/2025"],"award-info":[{"award-number":["UIDB\/00102\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UID\/04539\/2025"],"award-info":[{"award-number":["UID\/04539\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0058\/2020"],"award-info":[{"award-number":["LA\/P\/0058\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UID\/00285"],"award-info":[{"award-number":["UID\/00285"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0112\/2020"],"award-info":[{"award-number":["LA\/P\/0112\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["C644866475\u201300000012"],"award-info":[{"award-number":["C644866475\u201300000012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CEECIND\/01207\/2018"],"award-info":[{"award-number":["CEECIND\/01207\/2018"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["UIDB\/00102\/2025"],"award-info":[{"award-number":["UIDB\/00102\/2025"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["UID\/04539\/2025"],"award-info":[{"award-number":["UID\/04539\/2025"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["LA\/P\/0058\/2020"],"award-info":[{"award-number":["LA\/P\/0058\/2020"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["UID\/00285"],"award-info":[{"award-number":["UID\/00285"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["LA\/P\/0112\/2020"],"award-info":[{"award-number":["LA\/P\/0112\/2020"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]},{"award":["C644866475\u201300000012"],"award-info":[{"award-number":["C644866475\u201300000012"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Water scarcity is driving the development of strategies for treating municipal wastewater (MW) to enable its safe reuse. Nonetheless, MW contains contaminants of emerging concern (CECs), such as pharmaceuticals and antimicrobial-resistant (AMR) bacteria, which require innovative treatment technologies. In this context, Corbicula fluminea, an invasive freshwater clam, presents a high biofiltration capacity, and its environmental impact could be mitigated by assigning it a beneficial role in wastewater treatment. Methods: The ability of C. fluminea to remove chemical and biological CECs from real MW secondary-treated effluents was assessed. The effects of real wastewater on the clams\u2019 microbiome and on colony-forming unit (CFU) counts in their soft tissues were also assessed. Results: Under real conditions, the clams achieved over 73% removal for 3 chemical CECs after 24 h, with an average removal of approximately 39%. The clams showed recovery of both CFU counts and microbial community composition, dominated by opportunistic and stress-tolerant groups in the presence of pharmaceuticals. The removal of multidrug-resistant bacteria was evaluated; despite real wastewater reducing clearance rates, the clams significantly reduced these bacteria within 24 h. Conclusions: These results demonstrate that C. fluminea can serve as an effective polishing treatment, improving effluent quality, supporting control of this invasive species.<\/jats:p>","DOI":"10.3390\/ph19060870","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T01:08:02Z","timestamp":1780535282000},"page":"870","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biofiltration of Emerging Contaminants as a Sustainable Pest Management Strategy and Its Impact on Corbicula fluminea"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4777-4340","authenticated-orcid":false,"given":"Andr\u00e9 M. P. T.","family":"Pereira","sequence":"first","affiliation":[{"name":"LAQV, REQUIMTE, Laboratory of Bromatology and Pharmacognosy, Faculty of Pharmacy, University of Coimbra, Polo III, Azinhaga de St\u00aa Comba, 3000-548 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7412-8371","authenticated-orcid":false,"given":"Eva","family":"Domingues","sequence":"additional","affiliation":[{"name":"CERES, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Rua S\u00edlvio Lima, Polo II, 3030-790 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2372-1874","authenticated-orcid":false,"given":"Liliana J. G.","family":"Silva","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Laboratory of Bromatology and Pharmacognosy, Faculty of Pharmacy, University of Coimbra, Polo III, Azinhaga de St\u00aa Comba, 3000-548 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3292-5473","authenticated-orcid":false,"given":"Andreia","family":"Freitas","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Rua Dom Manuel II, Apartado 55142, 4051-401 Porto, Portugal"},{"name":"INIAV, I.P., Rua dos L\u00e1gidos, Lugar da Madalena, Vair\u00e3o, 4485-655 Vila do Conde, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1939-6389","authenticated-orcid":false,"given":"Paula V.","family":"Morais","sequence":"additional","affiliation":[{"name":"CEMMPRE, ARISE, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8879-5113","authenticated-orcid":false,"given":"Sara","family":"Domingues","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy of University of Coimbra, University Coimbra, Polo III, Azinhaga de St\u00aa Comba, 3000-458 Coimbra, Portugal"},{"name":"CNC-UC\u2014Center for Neuroscience and Cell Biology, University Coimbra, 3004-517 Coimbra, Portugal"},{"name":"CIBB\u2014Centre for Innovative Biomedicine and Biotechnology, University Coimbra, 3004-548 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4277-4365","authenticated-orcid":false,"given":"Tiago","family":"Lima","sequence":"additional","affiliation":[{"name":"Comprehensive Health Research Center (CHRC), University of \u00c9vora, 7004-516 \u00c9vora, Portugal"},{"name":"Department of Medical and Health Sciences, School of Health and Human Development, University of \u00c9vora, 7004-516 \u00c9vora, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7479-8540","authenticated-orcid":false,"given":"Gabriela J.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy of University of Coimbra, University Coimbra, Polo III, Azinhaga de St\u00aa Comba, 3000-458 Coimbra, Portugal"},{"name":"CNC-UC\u2014Center for Neuroscience and Cell Biology, University Coimbra, 3004-517 Coimbra, Portugal"},{"name":"CIBB\u2014Centre for Innovative Biomedicine and Biotechnology, University Coimbra, 3004-548 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Chung","sequence":"additional","affiliation":[{"name":"CEMMPRE, ARISE, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6087-1575","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Gomes","sequence":"additional","affiliation":[{"name":"CERES, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Rua S\u00edlvio Lima, Polo II, 3030-790 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.scitotenv.2017.01.216","article-title":"Application of ozonation for pharmaceuticals and personal care products removal from water","volume":"586","author":"Gomes","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100154","DOI":"10.1016\/j.wasec.2023.100154","article-title":"A review of water reuse applications and effluent standards in response to water scarcity","volume":"20","author":"Rich","year":"2023","journal-title":"Water Secur."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"159431","DOI":"10.1016\/j.scitotenv.2022.159431","article-title":"A review of freshwater benthic clams (Corbicula fluminea): Accumulation capacity, underlying physiological mechanisms and environmental applications","volume":"857","author":"Li","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Barka, E., Noutsopoulos, C., Galani, A., Panagou, I., Kalli, M., Koumaki, E., Malamis, S., and Mamais, D. (2023). Removal of Contaminants of Emerging Concern from Wastewater Using an Integrated Column System Containing Zero Valent Iron Nanoparticles. Water, 15.","DOI":"10.3390\/w15030598"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100105","DOI":"10.1016\/j.hazl.2024.100105","article-title":"Antibiotic pollution and associated antimicrobial resistance in the environment","volume":"5","author":"Barathe","year":"2024","journal-title":"J. Hazard. Mater. Lett."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"McEwen, S.A., and Collignon, P.J. (2018). Antimicrobial Resistance: A One Health Perspective. Antimicrobial Resistance in Bacteria from Livestock and Companion Animals, ASM Press.","DOI":"10.1128\/9781555819804.ch25"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.envint.2013.06.012","article-title":"Pharmaceuticals and personal care products (PPCPs): A review on environmental contamination in China","volume":"59","author":"Liu","year":"2013","journal-title":"Environ. Int."},{"key":"#cr-split#-ref_8.1","unstructured":"(2020). European Parliament and the Council of the European Union Regulation"},{"key":"#cr-split#-ref_8.2","unstructured":"(EU) 2020\/741. Off. J. Eur. Union, 32-55. Available online: https:\/\/eur-lex.europa.eu\/eli\/reg\/2020\/741\/oj\/eng."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1016\/j.ecolecon.2011.05.006","article-title":"The Asian clam Corbicula fluminea in the European freshwater-dependent industry: A latent threat or a friendly enemy?","volume":"70","author":"Rosa","year":"2011","journal-title":"Ecol. Econ."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"119773","DOI":"10.1016\/j.jclepro.2019.119773","article-title":"Potential of the bivalve Corbicula fluminea for the remediation of olive oil wastewaters","volume":"252","author":"Domingues","year":"2020","journal-title":"J. Clean. Prod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"102145","DOI":"10.1016\/j.jwpe.2021.102145","article-title":"Coagulation and biofiltration by Corbicula fluminea for COD and toxicity reduction of swine wastewater","volume":"42","author":"Gomes","year":"2021","journal-title":"J. Water Process Eng."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.scitotenv.2013.12.065","article-title":"A review on the occurrence of micropollutants in the aquatic environment and their fate and removal during wastewater treatment","volume":"473\u2013474","author":"Luo","year":"2014","journal-title":"Sci. Total Environ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.scitotenv.2017.06.022","article-title":"A critical evaluation of different parameters for estimating pharmaceutical exposure seeking an improved environmental risk assessment","volume":"603","author":"Pereira","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"114921","DOI":"10.1016\/j.watres.2019.114921","article-title":"Microalgal bioremediation of emerging contaminants-Opportunities and challenges","volume":"164","author":"Sutherland","year":"2019","journal-title":"Water Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Pereira, A., de Morais, E.G., Silva, L., Pena, A., Freitas, A., Teixeira, M.R., Varela, J., and Barreira, L. (2023). Pharmaceuticals Removal from Wastewater with Microalgae: A Pilot Study. Appl. Sci., 13.","DOI":"10.3390\/app13116414"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"253","DOI":"10.2166\/wh.2020.213","article-title":"Pharmaceuticals\u2019 removal by constructed wetlands: A critical evaluation and meta-analysis on performance, risk reduction, and role of physicochemical properties on removal mechanisms","volume":"18","author":"Ilyas","year":"2020","journal-title":"J. Water Health"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Berm\u00fadez, L.A., Pascual, J.M., Mart\u00ednez, M.d.M.M., and Poyatos Capilla, J.M. (2021). Effectiveness of Advanced Oxidation Processes in Wastewater Treatment: State of the Art. Water, 13.","DOI":"10.3390\/w13152094"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.ecoleng.2014.08.004","article-title":"The biofiltration process by the bivalve D. polymorpha for the removal of some pharmaceuticals and drugs of abuse from civil wastewaters","volume":"71","author":"Binelli","year":"2014","journal-title":"Ecol. Eng."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Pinto, I., Sim\u00f5es, M., and Gomes, I.B. (2022). An Overview of the Impact of Pharmaceuticals on Aquatic Microbial Communities. Antibiotics, 11.","DOI":"10.3390\/antibiotics11121700"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"\u015awiacka, K., Maculewicz, J., Kowalska, D., and Grace, M.R. (2023). Do pharmaceuticals affect microbial communities in aquatic environments? A review. Front. Environ. Sci., 10.","DOI":"10.3389\/fenvs.2022.1093920"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Makwarela, T.G., Seoraj-Pillai, N., and Nangammbi, T.C. (2025). Exploring the Molluscan Microbiome: Diversity, Function, and Ecological Implications. Biology, 14.","DOI":"10.3390\/biology14081086"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s40168-019-0714-6","article-title":"A pollution gradient contributes to the taxonomic, functional, and resistome diversity of microbial communities in marine sediments","volume":"7","author":"Chen","year":"2019","journal-title":"Microbiome"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"108805","DOI":"10.1016\/j.envint.2024.108805","article-title":"Diverse mechanisms by which chemical pollutant exposure alters gut microbiota metabolism and inflammation","volume":"190","author":"Teffera","year":"2024","journal-title":"Environ. Int."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1051\/limn:2008017","article-title":"Ecology of the invasive Asian clam Corbicula fluminea (M\u00fcller, 1774) in aquatic ecosystems: An overview","volume":"44","author":"Sousa","year":"2008","journal-title":"Ann. Limnol.-Int. J. Limnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e02328-21","DOI":"10.1128\/aem.02328-21","article-title":"Intrahabitat Differences in Bacterial Communities Associated with Corbicula fluminea in the Large Shallow Eutrophic Lake Taihu","volume":"88","author":"Zhao","year":"2022","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"118884","DOI":"10.1016\/j.jece.2025.118884","article-title":"The impact on the bacterial community of Corbicula fluminea after microcontaminants biofiltration","volume":"13","author":"Domingues","year":"2025","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Masanja, F., Yang, K., Xu, Y., He, G., Liu, X., Xu, X., Jiang, X., Luo, X., Mkuye, R., and Deng, Y. (2023). Bivalves and microbes: A mini-review of their relationship and potential implications for human health in a rapidly warming ocean. Front. Mar. Sci., 10.","DOI":"10.3389\/fmars.2023.1182438"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Mont\u00fafar-Romero, M., Valenzuela-Miranda, D., Valenzuela-Mu\u00f1oz, V., Morales-Rivera, M.F., and Gallardo-Esc\u00e1rate, C. (2025). Microbiota Dysbiosis in Mytilus chilensis Is Induced by Hypoxia, Leading to Molecular and Functional Consequences. Microorganisms, 13.","DOI":"10.20944\/preprints202502.1960.v1"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s10123-023-00397-3","article-title":"Gut microbiome resilience of green-lipped mussels, Perna canaliculus, to starvation","volume":"27","author":"Li","year":"2023","journal-title":"Int. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"104621","DOI":"10.1016\/j.jprot.2022.104621","article-title":"Quantitative proteomics reveals the antibiotics adaptation mechanism of Aeromonas hydrophila under kanamycin stress","volume":"264","author":"Zhang","year":"2022","journal-title":"J. Proteom."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s00253-014-5984-0","article-title":"Microbial community structure and pharmaceuticals and personal care products removal in a membrane bioreactor seeded with aerobic granular sludge","volume":"99","author":"Xia","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1006\/fmic.2002.0500","article-title":"Stress response of Aeromonas hydrophila following environmental challenges","volume":"19","author":"Isonhood","year":"2002","journal-title":"Food Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"137880","DOI":"10.1016\/j.jhazmat.2025.137880","article-title":"Responses of microbial community to the selection pressures of low-concentration contaminants of emerging concern in activated sludge","volume":"490","author":"Qin","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e02053-19","DOI":"10.1128\/AEM.02053-19","article-title":"Prevalence of Potentially Pathogenic Antibiotic-Resistant Aeromonas spp. in Treated Urban Wastewater Effluents versus Recipient Riverine Populations: A 3-Year Comparative Study","volume":"86","author":"Skwor","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Nascimento, M., Rodrigues, J., Matias, R., and Jordao, L. (2024). Aeromonas spp. in Freshwater Bodies: Antimicrobial Resistance and Biofilm Assembly. Antibiotics, 13.","DOI":"10.3390\/antibiotics13020166"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/s00253-024-13055-z","article-title":"Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing","volume":"108","author":"Neil","year":"2024","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Klim, M., \u017bmijowska, A., and Cyco\u0144, M. (2025). Potential of newly isolated strain Pseudomonas aeruginosa MC-1\/23 for the bioremediation of soil contaminated with selected non-steroidal anti-inflammatory drugs. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1542875"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.scitotenv.2018.02.095","article-title":"Toxicity assessment of pharmaceutical compounds on mixed culture from activated sludge using respirometric technique: The role of microbial community structure","volume":"630","author":"Vasiliadou","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s00253-012-4585-z","article-title":"Metabolic characteristics of the species Variovorax paradoxus","volume":"97","author":"Satola","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"105106","DOI":"10.1016\/j.jwpe.2024.105106","article-title":"Recent advances in microbial-assisted degradation and remediation of xenobiotic contaminants; challenges and future prospects","volume":"60","author":"Priya","year":"2024","journal-title":"J. Water Process Eng."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"154674","DOI":"10.1016\/j.scitotenv.2022.154674","article-title":"Fate and impact of wastewater-borne micropollutants in lettuce and the root-associated bacteria","volume":"831","author":"Bigott","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1007\/s11274-016-2012-9","article-title":"Bacterial communities in full-scale wastewater treatment systems","volume":"32","year":"2016","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/s41589-022-01237-7","article-title":"Complex regulation in a Comamonas platform for diverse aromatic carbon metabolism","volume":"19","author":"Wilkes","year":"2023","journal-title":"Nat. Chem. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.jes.2017.03.001","article-title":"Effects of residual organics in municipal wastewater on hydrogenotrophic denitrifying microbial communities","volume":"65","author":"Xing","year":"2018","journal-title":"J. Environ. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1007\/s00284-018-1466-4","article-title":"Community Structure Analysis and Biodegradation Potential of Aniline-Degrading Bacteria in Biofilters","volume":"75","author":"Hou","year":"2018","journal-title":"Curr. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1016\/j.chemosphere.2018.06.045","article-title":"Biofiltration using C. fluminea for E.coli removal from water: Comparison with ozonation and photocatalytic oxidation","volume":"208","author":"Gomes","year":"2018","journal-title":"Chemosphere"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.scitotenv.2015.09.007","article-title":"Removal of enteric viruses and Escherichia coli from municipal treated effluent by zebra mussels","volume":"539","author":"Mezzanotte","year":"2016","journal-title":"Sci. Total Environ."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1002\/jctb.5355","article-title":"Winery wastewater treatment by integrating Fenton\u2019s process with biofiltration by Corbicula fluminea","volume":"93","author":"Ferreira","year":"2018","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/BF00015510","article-title":"Feeding selectivity of Corbicula fluminea (Bivalvia) on natural phytoplankton","volume":"312","author":"Boltovskoy","year":"1995","journal-title":"Hydrobiologia"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Gomes, J., Domingues, E., Frasson, D., Martins, R.C., and Matos, A.M. (2024). Virus Removal from Real Wastewater as an Environmental Management Approach. Molecules, 29.","DOI":"10.3390\/molecules29235601"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Leston, S., Freitas, A., Rosa, J., Vila Pouca, A.S., Barbosa, J., Reis-Santos, P., Fonseca, V.F., Pardal, M.A., and Ramos, F. (2023). Pharmaceuticals in Coastal Waters: An UHPLC-TOF-MS Multi-Residue Approach. Appl. Sci., 13.","DOI":"10.3390\/app13105975"},{"key":"#cr-split#-ref_52.1","unstructured":"(2025). European Commission Commission Implementing Regulation"},{"key":"#cr-split#-ref_52.2","unstructured":"(EU) 2021\/808. Off. J. Eur. Union, 1-32. Available online: https:\/\/eur-lex.europa.eu\/eli\/reg_impl\/2021\/808\/oj\/eng."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/ismej.2011.82","article-title":"Impact of training sets on classification of high-throughput bacterial 16s rRNA gene surveys","volume":"6","author":"Werner","year":"2012","journal-title":"ISME J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s40168-018-0470-z","article-title":"Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2\u2019s q2-feature-classifier plugin","volume":"6","author":"Bokulich","year":"2018","journal-title":"Microbiome"},{"key":"ref_57","unstructured":"Bisanz, J. (2026, May 22). qiime2R: Importing QIIME2 Artifacts and Associated Data into R Sessions (Version 0.99). Available online: https:\/\/github.com\/jbisanz\/qiime2R\/."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"McMurdie, P.J., and Holmes, S. (2013). phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0061217"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.21105\/joss.01686","article-title":"Welcome to the Tidyverse","volume":"4","author":"Wickham","year":"2019","journal-title":"J. Open Source Softw."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Lima, T., Loureiro, D., Henriques, A., Ramos, F., Pomba, C., Domingues, S., and da Silva, G.J. (2022). Occurrence and Biological Cost of mcr-1-Carrying Plasmids Co-harbouring Beta-Lactamase Resistance Genes in Zoonotic Pathogens from Intensive Animal Production. Antibiotics, 11.","DOI":"10.3390\/antibiotics11101356"}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/6\/870\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T01:16:01Z","timestamp":1780535761000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/6\/870"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,30]]},"references-count":62,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ph19060870"],"URL":"https:\/\/doi.org\/10.3390\/ph19060870","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,30]]}}}