{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T07:04:26Z","timestamp":1784790266887,"version":"3.55.0"},"reference-count":29,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yozgat Bozok University Scientific Research Projects","award":["TED-2026-2342"],"award-info":[{"award-number":["TED-2026-2342"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Cannabinoid receptor type 2 (CB2) agonists have attracted attention because of their potential effects on tumor-related cellular processes in different cancer models. In the present study, we investigated the effects of the selective CB2 agonist JWH-133 on prostate cancer cell lines (LNCaP, DU-145, and PC3). Methods: Cell viability was evaluated using the MTT assay, while colony-forming capacity and migration-related responses were assessed by colony formation and wound-healing assays, respectively. In addition, the expression levels of selected cell-cycle- and apoptosis-related genes were analyzed by quantitative real-time PCR. Results: JWH-133 reduced cell viability in a time- and concentration-dependent manner, although the magnitude of this effect differed among prostate cancer cell lines. The compound also reduced colony formation in PC3 and LNCaP cells and decreased wound closure in PC3 cells under the experimental conditions used. Furthermore, JWH-133 altered the expression of several cell-cycle- and apoptosis-related genes in DU-145 and PC3 cells. Conclusions: Overall, these findings suggest that JWH-133 modulates multiple cellular responses in prostate cancer cells in a cell-line-dependent manner. However, additional mechanistic studies are required to clarify the molecular pathways underlying these effects.<\/jats:p>","DOI":"10.3390\/ph19071114","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:47:32Z","timestamp":1784508452000},"page":"1114","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selective CB2 Agonist JWH-133 Suppresses Viability and Migration-Related Responses in Prostate Cancer Cells"],"prefix":"10.3390","volume":"19","author":[{"given":"Seda Sabah","family":"\u00d6zcan","sequence":"first","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine, Manisa Celal Bayar University, Manisa 4500, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3117-5431","authenticated-orcid":false,"given":"Rehime","family":"Yapar","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine, Manisa Celal Bayar University, Manisa 4500, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6468-5091","authenticated-orcid":false,"given":"\u0130smail","family":"De\u011ferli","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine, Manisa Celal Bayar University, Manisa 4500, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levent","family":"Elmas","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine, \u0130zmir Bak\u0131r\u00e7ay University, Izmir 35660, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine, Manisa Celal Bayar University, Manisa 4500, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2066-829X","authenticated-orcid":false,"given":"Murat","family":"\u00c7ak\u0131r","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Yozgat Bozok University, Yozgat 66200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1001\/jama.2025.0228","article-title":"Prostate Cancer: A Review","volume":"333","author":"Raychaudhuri","year":"2025","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.14740\/wjon1191","article-title":"Epidemiology of Prostate Cancer","volume":"10","author":"Rawla","year":"2019","journal-title":"World J. 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