{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:57:49Z","timestamp":1785412669166,"version":"3.56.0"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002634","name":"Academic Research Fund of Hoseo University","doi-asserted-by":"publisher","award":["2025-0316-01"],"award-info":[{"award-number":["2025-0316-01"]}],"id":[{"id":"10.13039\/501100002634","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2025-0316-01"],"award-info":[{"award-number":["2025-0316-01"]}],"id":[{"id":"https:\/\/ror.org\/01qyd4k24","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Persicaria senticosa (Meisn.) H.Gross (PS) has anti-photoaging, anti-inflammatory, and antioxidant activities, but the anti-pigmentation potential of the PS flower absolute (PSFAb) remains largely unexplored. This study aimed to examine the chemical composition and anti-melanogenic and melanosome transport-inhibitory effects of PSFAb using B16BL6 murine melanoma cells. Methods: PSFAb was extracted with hexane and analyzed by gas chromatography\u2013mass spectrometry (GC-MS). The biological activities in B16BL6 murine melanoma cells were evaluated using water-soluble tetrazolium salt (WST) assays, 5-bromo-2\u2032-deoxyuridine (BrdU) incorporation, enzyme-linked immunosorbent assays, and immunoblotting methods. Results: GC-MS analysis identified eight constituents in PSFAb. Cell viability was not significantly altered in B16BL6 cells at concentrations \u2264 100 \u03bcg\/mL, which were used for additional tests. PSFAb inhibited serum-induced cell proliferation and suppressed \u03b1-melanocyte-stimulating hormone (\u03b1-MSH)-induced melanin synthesis and tyrosinase activity in B16BL6 cells. PSFAb also downregulated the \u03b1-MSH-induced expression of key melanogenic regulators, including microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2. PSFAb decreased extracellular signal-regulated kinase 1\/2 and p38 mitogen-activated protein kinase phosphorylation but enhanced JNK phosphorylation in \u03b1-MSH-stimulated B16BL6 cells. Furthermore, PSFAb reduced the \u03b1-MSH-induced expression of melanosome transport-related proteins (melanophilin and Rab27a) in B16BL6 cells. Conclusions: Overall, these results suggest that PSFAb has the potential to exert anti-pigmentation effects by suppressing melanogenesis and downregulating melanosome transport-related proteins. Therefore, PSFAb may be a promising candidate for the development of natural agents targeting hyperpigmentation and skin pigmentation regulation.<\/jats:p>","DOI":"10.3390\/ph19071129","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T10:48:28Z","timestamp":1784717308000},"page":"1129","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Composition and In Vitro Anti-Pigmentation Activity of Persicaria senticosa Flower Absolute: Potential Dual Inhibition of Melanogenesis and Melanosome Transport"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7677-4641","authenticated-orcid":false,"given":"Kyung Jong","family":"Won","sequence":"first","affiliation":[{"name":"Department of Physiology and Premedical Science, College of Medicine, Konkuk University, Chungju 27478, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3829-7893","authenticated-orcid":false,"given":"Hwan Myung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Bio-Health, Hoseo University, Asan 31499, Republic of Korea"},{"name":"Korea Essential Oil Resource Research Institute, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon Yi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Bio-Health, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hye","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Bio-Health, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Seong","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Bio-Health, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9607-6438","authenticated-orcid":false,"given":"Do Yoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Bio-Health, Hoseo University, Asan 31499, Republic of Korea"},{"name":"Korea Essential Oil Resource Research Institute, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1111\/j.1751-1097.2007.00226.x","article-title":"The protective role of melanin against UV damage in human skin","volume":"84","author":"Brenner","year":"2008","journal-title":"Photochem. 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