{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T05:01:28Z","timestamp":1784955688872,"version":"3.55.0"},"reference-count":54,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Protein kinases represent major therapeutic targets because dysregulation of their phosphorylation activity is associated with several diseases, including cancer, diabetes, and inflammatory disorders. Thus, many researchers in the pharmaceutical filed are making significant effort to design potent new protein kinase inhibitors (PKIs) as potential drugs. In this context, we aimed to identify new alternatives by exploiting the chemical space defined by ligands of the nucleotide-binding protein family for the discovery of novel protein kinase inhibitors. Protein kinases bind the nucleotide adenosine triphosphate (ATP) and belong to the nucleotide-binding protein group. Methods: All ligands of the nucleotide-binding protein family, excluding known kinase inhibitors, that were identified in the ChEMBL database were used in a structure-based virtual screening approach. From this set, we aimed to identify novel nucleotide-binding protein inhibitors (NBPIs) as novel kinase inhibitors. A total of 19,709 NBPI compounds that were dissimilar to known PKIs were docked on five protein kinases, and the 200 best scoring docking poses were retained for potential purchase. Results: Only 25 compounds were commercially available in stock and were evaluated experimentally on a panel of 10 diverse protein kinases. Three NBPI compounds, one of which had originally been identified as active against the ATP-binding cassette transporter ABCG2, were identified as Haspin kinase inhibitors with micromolar activity. Conclusions: This study presents an efficient computational approach to identifying novel kinase inhibitors from a database of ligands of the nucleotide-binding protein family, and the protocol could be applied to other protein target families.<\/jats:p>","DOI":"10.3390\/ph19081138","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T15:05:42Z","timestamp":1784819142000},"page":"1138","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Experimentally Validated Structure-Based Virtual Screening Approach to Identify Nucleotide-Binding Protein Inhibitors as a New Source of Kinase Inhibitors"],"prefix":"10.3390","volume":"19","author":[{"given":"Nicolas","family":"Bosc","sequence":"first","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), UMR CNRS-Universit\u00e9 d\u2019Orl\u00e9ans 7311, Universit\u00e9 d\u2019Orl\u00e9ans BP 6759, 45067 Orl\u00e9ans Cedex 2, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Carles","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), UMR CNRS-Universit\u00e9 d\u2019Orl\u00e9ans 7311, Universit\u00e9 d\u2019Orl\u00e9ans BP 6759, 45067 Orl\u00e9ans Cedex 2, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jade","family":"Fogha","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), UMR CNRS-Universit\u00e9 d\u2019Orl\u00e9ans 7311, Universit\u00e9 d\u2019Orl\u00e9ans BP 6759, 45067 Orl\u00e9ans Cedex 2, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blandine","family":"Baratte","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS FR2424, Plateforme de criblage KISSf (Kinase Inhibitor Specialized Screening facility) Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France"},{"name":"Sorbonne Universit\u00e9, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7186-7483","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bach","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, CNRS FR2424, Plateforme de criblage KISSf (Kinase Inhibitor Specialized Screening facility) Station Biologique, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France"},{"name":"Sorbonne Universit\u00e9, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2627-7924","authenticated-orcid":false,"given":"Samia","family":"Aci-S\u00e8che","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), UMR CNRS-Universit\u00e9 d\u2019Orl\u00e9ans 7311, Universit\u00e9 d\u2019Orl\u00e9ans BP 6759, 45067 Orl\u00e9ans Cedex 2, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9640-9665","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bourg","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), UMR CNRS-Universit\u00e9 d\u2019Orl\u00e9ans 7311, Universit\u00e9 d\u2019Orl\u00e9ans BP 6759, 45067 Orl\u00e9ans Cedex 2, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2823-0591","authenticated-orcid":false,"given":"Sylvain","family":"Routier","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique (ICOA), UMR CNRS-Universit\u00e9 d\u2019Orl\u00e9ans 7311, Universit\u00e9 d\u2019Orl\u00e9ans BP 6759, 45067 Orl\u00e9ans Cedex 2, 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Rev. Mol. Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/BF02740604","article-title":"Role of MAP kinase in neurons","volume":"16","author":"Fukunaga","year":"1998","journal-title":"Mol. Neurobiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S68","DOI":"10.2337\/diabetes.51.2007.S68","article-title":"\u03b2-Cell Protein Kinases and the Dynamics of the Insulin Response to Glucose","volume":"51","author":"Nesher","year":"2002","journal-title":"Diabetes"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1126\/science.1075762","article-title":"The Protein Kinase Complement of the Human Genome","volume":"298","author":"Manning","year":"2002","journal-title":"Science"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6818","DOI":"10.1073\/pnas.1814279116","article-title":"Defining a new nomenclature for the structures of active and inactive kinases","volume":"116","author":"Modi","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/nrd2907","article-title":"Cell cycle kinases as therapeutic targets for cancer","volume":"8","author":"Lapenna","year":"2009","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1517\/14728220903213426","article-title":"Targeting the JAK2\/STAT3 axis in Alzheimer\u2019s disease","volume":"13","author":"Chiba","year":"2009","journal-title":"Expert Opin. Ther. Targets"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/414799a","article-title":"Insulin signalling and the regulation of glucose and lipid metabolism","volume":"414","author":"Saltiel","year":"2001","journal-title":"Nature"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/nrd773","article-title":"Protein kinases\u2014The major drug targets of the twenty-first century?","volume":"1","author":"Cohen","year":"2002","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Carles, F., Bourg, S., Meyer, C., and Bonnet, P. (2018). PKIDB: A Curated, Annotated and Updated Database of Protein Kinase Inhibitors in Clinical Trials. Molecules, 23.","DOI":"10.3390\/molecules23040908"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Bournez, C., Carles, F., Peyrat, G., Aci-S\u00e8che, S., Bourg, S., Meyer, C., and Bonnet, P. (2020). Comparative Assessment of Protein Kinase Inhibitors in Public Databases and in PKIDB. Molecules, 25.","DOI":"10.3390\/molecules25143226"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1038\/d41573-025-00188-7","article-title":"FDA approves 100th small-molecule kinase inhibitor","volume":"24","author":"Mullard","year":"2025","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/nrd1468","article-title":"Drug repositioning: Identifying and developing new uses for existing drugs","volume":"3","author":"Ashburn","year":"2004","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1021\/cb5006323","article-title":"Identification and Structure--Function Analysis of Subfamily Selective G Protein-Coupled Receptor Kinase Inhibitors","volume":"10","author":"Homan","year":"2015","journal-title":"ACS Chem. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1002\/cmdc.201100410","article-title":"Nocodazole is a High-Affinity Ligand for the Cancer-Related Kinases ABL, c-KIT, BRAF, and MEK","volume":"7","author":"Park","year":"2012","journal-title":"ChemMedChem"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1021\/jm400045y","article-title":"A New Target for an Old Drug: Identifying Mitoxantrone as a Nanomolar Inhibitor of PIM1 Kinase via Kinome-Wide Selectivity Modeling","volume":"56","author":"Wan","year":"2013","journal-title":"J. Med. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"102247","DOI":"10.1016\/j.jbc.2022.102247","article-title":"Structural features of the protein kinase domain and targeted binding by small-molecule inhibitors","volume":"298","author":"Arter","year":"2022","journal-title":"J. Biol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Huang, L.C., Yeung, W., Wang, Y., Cheng, H., Venkat, A., Li, S., Ma, P., Rasheed, K., and Kannan, N. (2020). Quantitative Structure\u2013Mutation\u2013Activity Relationship Tests (QSMART) model for protein kinase inhibitor response prediction. BMC Bioinform., 21.","DOI":"10.1186\/s12859-020-03842-6"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ion, G.N.D., and Nitulescu, G.M. (2020). In Search of Outliers. Mining for Protein Kinase Inhibitors Based on Their Anti-Proliferative NCI-60 Cell Lines Profile. Molecules, 25.","DOI":"10.3390\/molecules25081766"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1038\/s41598-024-52413-9","article-title":"Network pharmacology combined with experimental verification to explore the potential mechanism of naringenin in the treatment of cervical cancer","volume":"14","author":"Zhou","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"129742","DOI":"10.1016\/j.bmcl.2024.129742","article-title":"Discovery of novel nucleoside derivatives as selective lysine acetyltransferase p300 inhibitors for cancer therapy","volume":"104","author":"Dai","year":"2024","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"D1180","DOI":"10.1093\/nar\/gkad1004","article-title":"The ChEMBL Database in 2023: A drug discovery platform spanning multiple bioactivity data types and time periods","volume":"52","author":"Zdrazil","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13264","DOI":"10.1021\/acs.jmedchem.2c01106","article-title":"Development and characterization of Type I, Type II, and Type III LIM-kinase chemical probes","volume":"65","author":"Hanke","year":"2022","journal-title":"J. Med. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7841","DOI":"10.1021\/jm3008954","article-title":"Crystal Structure of Human Aurora B in Complex with INCENP and VX-680","volume":"55","author":"Elkins","year":"2012","journal-title":"J. Med. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1016\/j.chembiol.2012.06.015","article-title":"An Integrated Chemical Biology Approach Provides Insight into Cdk2 Functional Redundancy and Inhibitor Sensitivity","volume":"19","author":"Echalier","year":"2012","journal-title":"Chem. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/j.chembiol.2005.05.011","article-title":"Defining Cdk5 Ligand Chemical Space with Small Molecule Inhibitors of Tau Phosphorylation","volume":"12","author":"Ahn","year":"2005","journal-title":"Chem. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.chembiol.2010.11.009","article-title":"Specific CLK inhibitors from a novel chemotype for regulation of alternative splicing","volume":"18","author":"Fedorov","year":"2011","journal-title":"Chem. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1016\/j.str.2013.03.012","article-title":"Structures of Down syndrome kinases, DYRKs, reveal mechanisms of kinase activation and substrate recognition","volume":"21","author":"Soundararajan","year":"2013","journal-title":"Structure"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Bosc, N., Meyer, C., and Bonnet, P. (2017). The use of novel selectivity metrics in kinase research. BMC Bioinform., 18.","DOI":"10.1186\/s12859-016-1413-y"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/gb4184","article-title":"Cyclin-dependent kinases","volume":"15","author":"Malumbres","year":"2014","journal-title":"Genome Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.ejmech.2013.06.035","article-title":"Synthesis and biological evaluation of flavones and benzoflavones as inhibitors of BCRP\/ABCG2","volume":"67","author":"Juvale","year":"2013","journal-title":"Eur. J. Med. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2894","DOI":"10.1073\/pnas.1917635117","article-title":"A precisely positioned MED12 activation helix stimulates CDK8 kinase activity","volume":"117","author":"Klatt","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"D331","DOI":"10.1093\/nar\/gkw1108","article-title":"Expansion of the Gene Ontology knowledgebase and resources","volume":"45","author":"Consortium","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"The UniProt Consortium (2025). UniProt: The Universal Protein Knowledgebase in 2025. Nucleic Acids Res., 53, D609\u2013D617.","DOI":"10.1093\/nar\/gkae1010"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"The UniProt Consortium (2019). UniProt: A worldwide hub of protein knowledge. Nucleic Acids Res., 47, D506\u2013D515.","DOI":"10.1093\/nar\/gky1049"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Preisach, C., Burkhardt, H., Schmidt-Thieme, L., and Decker, R. (2008). KNIME: The Konstanz Information Miner. Data Analysis, Machine Learning and Applications. Studies in Classification, Data Analysis, and Knowledge Organization, Springer.","DOI":"10.1007\/978-3-540-78246-9"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1021\/acs.jmedchem.5c00131","article-title":"Quantitation and Error Measurements in Dose\u2013Response Curves","volume":"68","author":"Srinivasan","year":"2025","journal-title":"J. Med. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(96)00423-1","article-title":"Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings","volume":"23","author":"Lipinski","year":"1997","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1021\/jm020017n","article-title":"Molecular Properties That Influence the Oral Bioavailability of Drug Candidates","volume":"45","author":"Veber","year":"2002","journal-title":"J. Med. Chem."},{"key":"ref_41","unstructured":"Chemical Computing Group Inc. (2026). Molecular Operating Environment (MOE), Chemical Computing Group ULC. 2024.0601."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1002\/minf.201100076","article-title":"KNIME Workflow to Assess PAINS Filters in SMARTS Format. Comparison of RDKit and Indigo Cheminformatics Libraries","volume":"30","author":"Saubern","year":"2011","journal-title":"Mol. Inform."},{"key":"ref_43","first-page":"2825","article-title":"Scikit-learn: Machine learning in Python","volume":"12","author":"Pedregosa","year":"2011","journal-title":"J. Mach. Learn. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"108107","DOI":"10.1016\/j.phrs.2026.108107","article-title":"Properties of FDA-approved small molecule protein kinase inhibitors: A 2026 update","volume":"224","author":"Roskoski","year":"2026","journal-title":"Pharmacol. Res."},{"key":"ref_45","unstructured":"(2016, September 01). RDKit: Open-Source Cheminformatics. Available online: http:\/\/www.rdkit.org."},{"key":"ref_46","unstructured":"(2013). Glide, Schr\u00f6dinger LLC."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","article-title":"The Protein Data Bank","volume":"28","author":"Berman","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s10822-013-9644-8","article-title":"Protein and ligand preparation: Parameters, protocols, and influence on virtual screening enrichments","volume":"27","author":"Adzhigirey","year":"2013","journal-title":"J. Comput.-Aided Mol. Des."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1021\/jm0306430","article-title":"Glide: A New Approach for Rapid, Accurate Docking and Scoring. 1. Method and Assessment of Docking Accuracy","volume":"47","author":"Friesner","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1021\/jm051256o","article-title":"Extra Precision Glide: Docking and Scoring Incorporating a Model of Hydrophobic Enclosure for Protein\u2013Ligand Complexes","volume":"49","author":"Friesner","year":"2006","journal-title":"J. Med. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1038\/emboj.2008.121","article-title":"The structure of P-TEFb (CDK9\/cyclin T1), its complex with flavopiridol and regulation by phosphorylation","volume":"27","author":"Baumli","year":"2008","journal-title":"EMBO J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1038\/nature16979","article-title":"Structural basis of lenalidomide-induced CK1\u03b1degradation by the CRL4CRBN ubiquitin ligase","volume":"532","author":"Petzold","year":"2016","journal-title":"Nature"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.1074\/mcp.M113.034819","article-title":"Modulation of the chromatin phosphoproteome by the Haspin protein kinase","volume":"13","author":"Maiolica","year":"2014","journal-title":"Mol. Cell. Proteom."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"31208","DOI":"10.1074\/jbc.M500806200","article-title":"Roscovitine targets, protein kinases and pyridoxal kinase","volume":"280","author":"Bach","year":"2005","journal-title":"J. Biol. Chem."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/8\/1138\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T04:15:54Z","timestamp":1784952954000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/8\/1138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,23]]},"references-count":54,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ph19081138"],"URL":"https:\/\/doi.org\/10.3390\/ph19081138","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,23]]}}}