{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T04:47:23Z","timestamp":1785905243593,"version":"3.56.0"},"reference-count":67,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Youth Promotion Project of the Guangdong Basic and Applied Basic Research Foundation","award":["2023A1515030127"],"award-info":[{"award-number":["2023A1515030127"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81804137"],"award-info":[{"award-number":["81804137"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["81804137"],"award-info":[{"award-number":["81804137"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Basic Research Project of the Guangzhou Science and Technology Bureau","award":["2023A03J0368"],"award-info":[{"award-number":["2023A03J0368"]}]},{"name":"Basic Research Project of the Guangzhou Science and Technology Bureau","award":["202201011635"],"award-info":[{"award-number":["202201011635"]}]},{"DOI":"10.13039\/100009659","name":"Guangzhou Medical University","doi-asserted-by":"crossref","award":["GMUCR2025-02012"],"award-info":[{"award-number":["GMUCR2025-02012"]}],"id":[{"id":"10.13039\/100009659","id-type":"DOI","asserted-by":"crossref"}]},{"award":["GMUCR2025-02012"],"award-info":[{"award-number":["GMUCR2025-02012"]}],"id":[{"id":"https:\/\/ror.org\/00zat6v61","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Uterine dysfunction contributes to infertility in PCOS. Jiawei Qi Gong Wan (JQGW) is used to improve endometrial homeostasis, but its mechanism is unclear. This study investigated whether JQGW improves uterine function via gut microbiota and metabolites. Methods: Letrozole-induced PCOS mice received JQGW (low\/high dose), metformin, or vehicle for 35 days. Endometrial morphology, receptivity genes, Akt2\/NF-\u03baB signaling, gut microbiota (16S rRNA), and serum metabolites (LC-MS) were assessed. Results: PCOS mice showed reduced endometrial thickness (126.4 \u00b1 10.8 \u03bcm vs. 215.6 \u00b1 12.3 \u03bcm in controls, p &lt; 0.001) and fewer glands (12.6 \u00b1 1.8 vs. 28.4 \u00b1 2.1, p &lt; 0.001). JQGW-H increased endometrial thickness (189.3 \u00b1 11.2 \u03bcm, p &lt; 0.01 vs. PCOS) and gland number (23.1 \u00b1 1.9, p &lt; 0.01 vs. PCOS), restored the receptivity markers (Nr2f2, Pc6, Ptch, and Hbegf) toward normal levels, suppressed Akt2\/NF-\u03baB activation, and reduced inflammatory cytokines. JQGW shifted the \u03b2-diversity structure of the gut microbiota toward the control pattern, with Oscillospira enrichment (LDA &gt; 4). Four metabolites (PA(20:0\/16:1(9Z)), 7-methylguanosine, methoxyacetic acid, 8.11-eicosadiynoic acid) showed nominal elevation in PCOS and negative correlations with endometrial thickness (r = \u22120.73 to \u22120.89, unadjusted p &lt; 0.01), although none survived FDR correction. Conclusions: JQGW ameliorates PCOS-associated uterine dysfunction, potentially via gut microbiota and metabolite modulation. Future studies should validate causality using fertility-based outcomes and microbiota transplantation.<\/jats:p>","DOI":"10.3390\/ph19081153","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T01:26:23Z","timestamp":1785115583000},"page":"1153","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Jiawei Qi Gong Wan Improves Endometrial Receptivity in PCOS-like Mice by Attenuating Inflammation: A Multi-Omics Study Linking Gut Microbiota and Metabolite Profiles"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8498-8869","authenticated-orcid":false,"given":"Ruqun","family":"Zheng","sequence":"first","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"},{"name":"Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Song","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies, Department of Laboratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingyan","family":"Shen","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiqi","family":"Liu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjia","family":"Shi","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Zhuo","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyu","family":"Luo","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, National Clinical Research Center of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4477-613X","authenticated-orcid":false,"given":"Hongxia","family":"Ma","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Hu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3928-7278","authenticated-orcid":false,"given":"Chi Chiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong, China"},{"name":"Li Ka Shing Institute of Health Sciences, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China"},{"name":"The Chinese University of Hong Kong-Sichuan University Joint Laboratory for Reproductive Medicine, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Advanced Interdisciplinary Studies Center, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.fertnstert.2023.07.025","article-title":"Recommendations from the 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