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Its potential hepatotoxicity has raised concern, although the associated molecular alterations remain incompletely understood. Chlorogenic acid (CGA), a naturally occurring polyphenol, has been reported to affect oxidative stress and metabolic homeostasis. Methods: Adult male C57BL\/6J mice were exposed to GenX (2 mg\/kg\/day) with or without CGA (30 mg\/kg\/day) by gavage for 12 weeks. AML12 cells were treated with GenX (10\u2013800 \u03bcM) for 24 or 48 h to assess cell viability, and intracellular lipid accumulation was evaluated after exposure to 200 \u03bcM GenX for 24 h. Results: GenX exposure induced hepatomegaly, microvesicular steatosis, inflammatory cell infiltration, and a reduction in hepatic glycogen stores. It also decreased hepatic glutathione concentrations and increased hepatic malondialdehyde concentrations. Serum alanine aminotransferase, aspartate aminotransferase, total cholesterol, and triglyceride levels were elevated. In AML12 cells, GenX increased intracellular lipid accumulation, as assessed by Oil Red O staining. Transcriptomic analysis identified significant enrichment of the peroxisome proliferator-activated receptor (PPAR) signaling pathway. Consistently, GenX altered the expression of genes and proteins involved in lipogenesis, fatty acid uptake, lipid storage, and fatty acid oxidation, suggesting disturbed PPAR-related lipid metabolic regulation. Moreover, the decreased p-mTOR\/mTOR ratio, increased LC3-II\/I, and overexpression of Beclin1, p62, and inflammatory mediators in the GenX group might suggest changes in autophagy-related proteins and inflammatory response. CGA coadministration partially attenuated several GenX-induced hepatic alterations, including liver enlargement, hepatic lipid accumulation, lipid peroxidation, and changes in selected autophagy- and inflammation-related proteins. Conclusions: Subchronic GenX exposure-induced adverse hepatic effects might be associated with disrupted PPAR-related lipid metabolic regulation, oxidative stress, inflammatory responses, and changes in autophagy-related proteins. CGA might exert potential modulatory effects.<\/jats:p>","DOI":"10.3390\/ph19081164","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T01:26:23Z","timestamp":1785115583000},"page":"1164","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Subchronic GenX Exposure Induces Hepatic Alterations Accompanied by Changes in PPAR-Related Lipid Metabolism and Autophagy-Related Proteins in Adult Male C57BL\/6J Mice: Partial Attenuation by Chlorogenic Acid"],"prefix":"10.3390","volume":"19","author":[{"given":"Jinjin","family":"Zhang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Toxicology, School of Public Health, Southern Medical University, No. 1838 North Guangzhou Road, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Toxicology, School of Public Health, Southern Medical University, No. 1838 North Guangzhou Road, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Yukui","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Toxicology, School of Public Health, Southern Medical University, No. 1838 North Guangzhou Road, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1556-2167","authenticated-orcid":false,"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Forensic Pathology, School of Forensic Medicine, Southern Medical University, No. 1838 North Guangzhou Road, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5621-9250","authenticated-orcid":false,"given":"Xiao-Li","family":"Xie","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Toxicology, School of Public Health, Southern Medical University, No. 1838 North Guangzhou Road, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"127701","DOI":"10.1289\/EHP14653","article-title":"Examining Potential PFAS Contamination of Private Wells from a High School in Rural Maine","volume":"132","author":"Sosa","year":"2024","journal-title":"Environ. Health Perspect."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"120482","DOI":"10.1016\/j.envres.2024.120482","article-title":"Spatial variations in per- and polyfluoroalkyl substance concentrations in pooled sera from inland, coastal, and island populations","volume":"266","author":"Orr","year":"2024","journal-title":"Environ. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"108703","DOI":"10.1016\/j.envint.2024.108703","article-title":"Sub-acute exposure of male guppies (Poecilia reticulata) to environmentally relevant concentrations of PFOA and GenX induces significant changes in the testis transcriptome and reproductive traits","volume":"187","author":"Gaspar","year":"2024","journal-title":"Environ. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"113467","DOI":"10.1016\/j.ecoenv.2022.113467","article-title":"Perfluorooctanoic acid alters the developmental trajectory of female germ cells and embryos in rodents and its potential mechanism","volume":"236","author":"Huang","year":"2022","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"153281","DOI":"10.1016\/j.scitotenv.2022.153281","article-title":"Gestational exposure to GenX induces hepatic alterations by the gut-liver axis in maternal mice: A similar mechanism as PFOA","volume":"820","author":"Xu","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"123090","DOI":"10.1016\/j.envpol.2023.123090","article-title":"Inulin alleviates perfluorooctanoic acid-induced intestinal injury in mice by modulating the PI3K\/AKT\/mTOR signaling pathway","volume":"342","author":"Zhang","year":"2024","journal-title":"Environ. Pollut."},{"key":"ref_7","unstructured":"UNEP (2024, December 22). Listing of Perfluorooctanoic Acid (PFOA), Its Salts and PFOA-Related Compounds. Available online: https:\/\/www.informea.org\/zh-hans\/decision\/listing-perfluorooctanoic-acid-pfoa-its-salts-and-pfoa-related-compounds-advance."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"27006","DOI":"10.1289\/EHP6233","article-title":"Evaluation of Maternal, Embryo, and Placental Effects in CD-1 Mice following Gestational Exposure to Perfluorooctanoic Acid (PFOA) or Hexafluoropropylene Oxide Dimer Acid (HFPO-DA or GenX)","volume":"128","author":"Blake","year":"2020","journal-title":"Environ. Health Perspect."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"106204","DOI":"10.1016\/j.envint.2020.106204","article-title":"Hexafluoropropylene oxide-dimer acid (HFPO-DA or GenX) alters maternal and fetal glucose and lipid metabolism and produces neonatal mortality, low birthweight, and hepatomegaly in the Sprague-Dawley rat","volume":"146","author":"Conley","year":"2021","journal-title":"Environ. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"106843","DOI":"10.1016\/j.envint.2021.106843","article-title":"Exposure to a mixture of legacy, alternative, and replacement per- and polyfluoroalkyl substances (PFAS) results in sex-dependent modulation of cholesterol metabolism and liver injury","volume":"157","author":"Roth","year":"2021","journal-title":"Environ. Int."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Yoo, H.J., Pyo, M.C., Park, Y., Kim, B.Y., and Lee, K.W. (2021). Hexafluoropropylene oxide dimer acid (GenX) exposure induces apoptosis in HepG2 cells. Heliyon, 7.","DOI":"10.2139\/ssrn.3873684"},{"key":"ref_12","unstructured":"McCloskey, H. (2024, November 15). A Closer Look at PFAS Drinking Water Regulations. Available online: https:\/\/collaboratory.unc.edu\/news\/2025\/01\/17\/a-closer-look-at-pfas-drinking-water-regulations\/."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhao, H., Li, Z., Sun, Y., Yan, M., Wang, Y., Li, Y., Zhang, Y., and Zhu, M. (2024). Supplementation of Chlorogenic Acid Alleviates the Effects of H2O2-Induced Oxidative Stress on Laying Performance, Egg Quality, Antioxidant Capacity, Hepatic Inflammation, Mitochondrial Dysfunction, and Lipid Accumulation in Laying Hens. Antioxidants, 13.","DOI":"10.3390\/antiox13111303"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1111\/jpn.13905","article-title":"Bioactive functions of chlorogenic acid and its research progress in pig industry","volume":"108","author":"Dai","year":"2024","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1089\/ars.2023.0254","article-title":"A Systematic Review and Comprehensive Evaluation of Human Intervention Studies to Unravel the Bioavailability of Hydroxycinnamic Acids","volume":"40","author":"Di","year":"2024","journal-title":"Antioxid. Redox Signal."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"112393","DOI":"10.1016\/j.foodres.2022.112393","article-title":"Characterization of procyanidin extracts from hawthorn (Crataegus pinnatifida) in human colorectal adenocarcinoma cell line Caco-2, simulated Digestion, and fermentation identified unique and novel prebiotic properties","volume":"165","author":"Han","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ma, X., Xiong, S., Xiang, F., Li, Y., Lin, Y., Liu, Y., Lin, L., and Xie, J. (2025). The Protective Effect of Phenolic Acids on Liver Disease: A Review of Possible Mechanisms. Antioxidants, 14.","DOI":"10.3390\/antiox14101247"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.biochi.2018.05.008","article-title":"Chlorogenic acid ameliorates alcohol-induced liver injuries through scavenging reactive oxygen species","volume":"150","author":"Kim","year":"2018","journal-title":"Biochimie"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/j.fct.2010.01.003","article-title":"Chlorogenic acid exhibits anti-obesity property and improves lipid metabolism in high-fat diet-induced-obese mice","volume":"48","author":"Cho","year":"2010","journal-title":"Food Chem. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wang, Q., Liu, T., Koci, M., Wang, Y., Fu, Y., Ma, M., Ma, Q., and Zhao, L. (2023). Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2\/HO-1, and Inhibiting Noncanonical NF-\u03baB Signaling Pathway. Antioxidants, 12.","DOI":"10.3390\/antiox12122027"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.emcon.2021.10.004","article-title":"Latent, sex-specific metabolic health effects in CD-1 mouse offspring exposed to PFOA or HFPO-DA (GenX) during gestation","volume":"7","author":"Cope","year":"2021","journal-title":"Emerg. Contam."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"118202","DOI":"10.1016\/j.envpol.2021.118202","article-title":"Exposure to GenX and its novel analogs disrupts fatty acid metabolism in male mice","volume":"291","author":"Guo","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"101602","DOI":"10.1016\/j.molmet.2022.101602","article-title":"Exposure to low-dose perfluorooctanoic acid promotes hepatic steatosis and disrupts the hepatic transcriptome in mice","volume":"66","author":"Attema","year":"2022","journal-title":"Mol. Metab."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"116830","DOI":"10.1016\/j.jpba.2025.116830","article-title":"Black rice bran improved lipid metabolism in hyperlipidemia mice via steroid hormone biosynthesis and PPAR signaling pathway","volume":"262","author":"Li","year":"2025","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.jhep.2021.09.039","article-title":"Exposure to environmental contaminants is associated with altered hepatic lipid metabolism in non-alcoholic fatty liver disease","volume":"76","author":"Sen","year":"2022","journal-title":"J. Hepatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"46001","DOI":"10.1289\/EHP10092","article-title":"Exposure to per- and Polyfluoroalkyl Substances and Markers of Liver Injury: A Systematic Review and Meta-Analysis","volume":"130","author":"Coste","year":"2022","journal-title":"Environ. Health Perspect."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"107779","DOI":"10.1016\/j.envint.2023.107779","article-title":"Serum per- and polyfluoroalkyl substances and abnormal lipid metabolism: A nationally representative cross-sectional study","volume":"172","author":"Wu","year":"2023","journal-title":"Environ. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"115020","DOI":"10.1016\/j.ecoenv.2023.115020","article-title":"Exposure to PFOA and its novel analogs disrupts lipid metabolism in zebrafish","volume":"259","author":"Sun","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lange, F., Graf, V., Caussy, C., and Dufour, J.F. (2022). PPAR-Targeted Therapies in the Treatment of Non-Alcoholic Fatty Liver Disease in Diabetic Patients. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23084305"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"154338","DOI":"10.1016\/j.metabol.2020.154338","article-title":"The role of peroxisome proliferator-activated receptors (PPAR) in immune responses","volume":"114","author":"Chrisdes","year":"2021","journal-title":"Metabolism"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wang, Y., Nakajima, T., Gonzalez, F.J., and Tanaka, N. (2020). PPARs as Metabolic Regulators in the Liver: Lessons from Liver-Specific PPAR-Null Mice. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21062061"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.freeradbiomed.2021.08.005","article-title":"Activation of PPAR\u03b1-catalase pathway reverses alcoholic liver injury via upregulating NAD synthesis and accelerating alcohol clearance","volume":"174","author":"Yue","year":"2021","journal-title":"Free Radic. Biol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1269747","DOI":"10.1155\/2022\/1269747","article-title":"PPAR\u03b3 Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS\/TXNIP\/NLRP3 Signaling Pathway","volume":"2022","author":"Li","year":"2022","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.1007\/s00204-024-03789-0","article-title":"Identifying novel mechanisms of per- and polyfluoroalkyl substance-induced hepatotoxicity using FRG humanized mice","volume":"98","author":"Robarts","year":"2024","journal-title":"Arch. Toxicol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"123314","DOI":"10.1016\/j.envpol.2024.123314","article-title":"GenX analogs exposure induced greater hepatotoxicity than GenX mainly via activation of PPAR\u03b1 pathway while caused hepatomegaly in the absence of PPAR\u03b1 in female mice","volume":"344","author":"Ren","year":"2024","journal-title":"Environ. Pollut."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2317","DOI":"10.7150\/ijbs.69609","article-title":"Disruption of peroxisome proliferator-activated receptor \u03b1 in hepatocytes protects against acetaminophen-induced liver injury by activating the IL-6\/STAT3 pathway","volume":"18","author":"Zhang","year":"2022","journal-title":"Int. J. Biol. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"829","DOI":"10.4093\/dmj.2021.0274","article-title":"Peroxisomal Fitness: A Potential Protective Mechanism of Fenofibrate against High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in Mice","volume":"46","author":"Jiang","year":"2022","journal-title":"Diabetes Metab. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1177\/0192623320905803","article-title":"Assessment of the Mode of Action Underlying the Effects of GenX in Mouse Liver and Implications for Assessing Human Health Risks","volume":"48","author":"Chapp","year":"2020","journal-title":"Toxicol. Pathol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/s41423-025-01257-y","article-title":"Peroxisome proliferator-activated receptor alpha is an essential factor in enhanced macrophage immune function induced by angiotensin-converting enzyme","volume":"22","author":"Saito","year":"2025","journal-title":"Cell. Mol. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"108138","DOI":"10.1016\/j.envint.2023.108138","article-title":"PPAR\u03b1\/ACOX1 as a novel target for hepatic lipid metabolism disorders induced by per- and polyfluoroalkyl substances: An integrated approach","volume":"178","author":"Yang","year":"2023","journal-title":"Environ. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1161\/CIRCRESAHA.117.311401","article-title":"Reactive Oxygen Species in Metabolic and Inflammatory Signaling","volume":"122","author":"Forre","year":"2018","journal-title":"Circ. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1152\/physrev.00026.2013","article-title":"Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release","volume":"94","author":"Zorov","year":"2014","journal-title":"Physiol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1038\/s41467-025-56666-4","article-title":"ER-mitochondria contacts mediate lipid radical transfer via RMDN3\/PTPIP51 phosphorylation to reduce mitochondrial oxidative stress","volume":"16","author":"Shiiba","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.7150\/thno.50905","article-title":"Mitochondrial ROS promote mitochondrial dysfunction and inflammation in ischemic acute kidney injury by disrupting TFAM-mediated mtDNA maintenance","volume":"11","author":"Zhao","year":"2021","journal-title":"Theranostics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"160085","DOI":"10.1016\/j.scitotenv.2022.160085","article-title":"Impacts of PFOA(C8), GenX(C6), and their mixtures on zebrafish developmental toxicity and gene expression provide insight about tumor-related disease","volume":"858","author":"Liu","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1080\/15548627.2015.1106668","article-title":"MTOR, PIK3C3, and autophagy: Signaling the beginning from the end","volume":"11","author":"Munson","year":"2015","journal-title":"Autophagy"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"114358","DOI":"10.1016\/j.expneurol.2023.114358","article-title":"UBE3A deficiency-induced autophagy is associated with activation of AMPK-ULK1 and p53 pathways","volume":"363","author":"Hao","year":"2023","journal-title":"Exp. Neurol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.3892\/mmr.2017.6828","article-title":"Wnt\/\u03b2-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line","volume":"16","author":"Tao","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.clbc.2021.07.015","article-title":"Radix Tetrastigma Extracts Enhance the Chemosensitivity in Triple-Negative Breast Cancer Via Inhibiting PI3K\/Akt\/mTOR-Mediated Autophagy","volume":"22","author":"Zhang","year":"2022","journal-title":"Clin. Breast Cancer"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1083\/jcb.200507002","article-title":"p62\/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death","volume":"171","author":"Lamark","year":"2005","journal-title":"J. Cell Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"105005","DOI":"10.1016\/j.psj.2025.105005","article-title":"Chlorogenic acid mitigates avian pathogenic Escherichia coli-induced intestinal barrier damage in broiler chickens via anti-inflammatory and antioxidant effects","volume":"104","author":"Wei","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Gao, L., Zhang, T., Zhang, X., Ding, W., Yang, J., Luo, Y., Wang, J., Yuan, X., and Zhao, Y. (2026). Research progress on the molecular mechanisms of chlorogenic acid\u2019s pharmacological effects and its advanced drug delivery systems. Front. Pharmacol., 17.","DOI":"10.3389\/fphar.2026.1746172"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"114314","DOI":"10.1016\/j.ecoenv.2022.114314","article-title":"Transcriptional pathways linked to fetal and maternal hepatic dysfunction caused by gestational exposure to perfluorooctanoic acid (PFOA) or hexafluoropropylene oxide-dimer acid (HFPO-DA or GenX) useCD-1 mice","volume":"248","author":"Blake","year":"2022","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"168059","DOI":"10.1016\/j.scitotenv.2023.168059","article-title":"Gestational GenX and PFOA exposures induce hepatotoxicity, metabolic pathway, and microbiome shifts in weanling mice","volume":"907","author":"Zhang","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"109020","DOI":"10.1016\/j.jnutbio.2022.109020","article-title":"Chlorogenic acid alleviated liver fibrosis in methionine and choline deficient diet-induced nonalcoholic steatohepatitis in mice and its mechanism","volume":"106","author":"Miao","year":"2022","journal-title":"J. Nutr. Biochem."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Yang, F., Luo, L., Zhu, Z.D., Zhou, X., Wang, Y., Xue, J., Zhang, J., Cai, X., Chen, Z.L., and Ma, Q. (2017). Chlorogenic Acid Inhibits Liver Fibrosis by Blocking the miR-21-Regulated TGF-\u03b21\/Smad7 Signaling Pathway in Vitro and in Vivo. Front. Pharmacol., 8.","DOI":"10.3389\/fphar.2017.00929"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1002\/hep.20701","article-title":"Design and validation of a histological scoring system for nonalcoholic fatty liver disease","volume":"41","author":"Klein","year":"2005","journal-title":"Hepatology"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/8\/1164\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T13:16:26Z","timestamp":1789391786000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/8\/1164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":61,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ph19081164"],"URL":"https:\/\/doi.org\/10.3390\/ph19081164","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]}}}