{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:27:35Z","timestamp":1786987655523,"version":"build-2736575974"},"reference-count":47,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Diabetes mellitus is known to exacerbate neural degeneration and impair regenerative capacity following injury. This study aimed to investigate the effects of Garcinia kola and curcumin on spinal cord morphology and neural structure in a diabetic sciatic nerve injury model. Methods: Spinal cord tissues were obtained from male Wistar albino rats subjected to sciatic nerve transection and diabetes induction in a previously established experimental model. Animals had been assigned to five experimental groups (n = 7 per group): control group, transected sciatic nerve group, transected sciatic nerve + diabetes mellitus (DM), transected sciatic nerve + diabetes mellitus + curcumin (DM + Cur), and transected sciatic nerve + diabetes mellitus + Garcinia kola (DM + GK). Curcumin (300 mg\/kg\/day) and Garcinia kola (200 mg\/kg\/day) were administered orally. All animals were sacrificed at three months post-injury. Cervical (C3\u2013C5) spinal cord segments were processed for histological evaluation, and quantitative assessments were performed using unbiased stereological methods to estimate neuronal and structural parameters. Microscopic analyses were conducted to assess morphological alterations. Results: Diabetes combined with sciatic nerve transection induced marked structural alterations in the spinal cord, including reductions in neuronal density and disruption of tissue organization. The DM group showed a significantly lower number of motor neurons than the other groups; similarly, it showed a significant decrease in soma area compared to the Cont and Sham groups. Conclusions: Both the curcumin and Garcinia kola treatment groups exhibited partial preservation of spinal cord morphology. This study provides evidence that Garcinia kola and curcumin exert protective effects not only at the peripheral nerve level, but also in the spinal cord following diabetic nerve injury.<\/jats:p>","DOI":"10.3390\/ph19081301","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:35:08Z","timestamp":1786984508000},"page":"1301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role of Plant-Derived Compounds on the Cervical Spinal Cord After Sciatic Nerve Transection in Diabetic Rats"],"prefix":"10.3390","volume":"19","author":[{"given":"Abdullah Hilmi","family":"Marangoz","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Faculty of Medicine, Ondokuz May\u0131s University, 55139 Samsun, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6277-1255","authenticated-orcid":false,"given":"Burcu","family":"Deliba\u015f","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Recep Tayyip Erdo\u011fan University, 53200 Rize, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gamze","family":"Altun","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Ondokuz May\u0131s University, 55139 Samsun, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arife Ahsen","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Bolu Abant \u0130zzet Baysal University, 14030 Bolu, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1463-2757","authenticated-orcid":false,"given":"Hala Mahgoub","family":"Hamour","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Ondokuz May\u0131s University, 55139 Samsun, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1883-0021","authenticated-orcid":false,"given":"Abit","family":"Akta\u015f","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Medicine, Istanbul University, Cerrahpa\u015fa, 34320 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sait","family":"Polat","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, \u00c7ukurova University, 01330<bold\/> Adana, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1477-5002","authenticated-orcid":false,"given":"S\u00fcleyman","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Ondokuz May\u0131s University, 55139 Samsun, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102079","DOI":"10.1016\/j.jchemneu.2022.102079","article-title":"Spinal cord-wide structural disruption in type 2 diabetes rescued by exenatide, a glucagon-like peptide-1 analog, via down-regulating inflammatory, oxidative and apoptotic signaling pathways","volume":"121","author":"Mandour","year":"2022","journal-title":"J. Chem. Neuroanat."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/978-94-024-1079-2_30","article-title":"The Effect of Taurine and Its Immediate Homologs on Diabetes-Induced Oxidative Stress in the Brain and Spinal Cord of Rats","volume":"975","author":"Patel","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"106062","DOI":"10.1016\/j.phrs.2022.106062","article-title":"Cognitive impairment and type 2 diabetes mellitus: Focus of SGLT2 inhibitors treatment","volume":"176","author":"Rizzo","year":"2022","journal-title":"Pharmacol. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"102158","DOI":"10.1155\/2014\/102158","article-title":"Diabetes and the brain: Oxidative stress, inflammation, and autophagy","volume":"2014","author":"Muriach","year":"2014","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1210\/er.2003-0019","article-title":"Oxidative stress in the pathogenesis of diabetic neuropathy","volume":"25","author":"Vincent","year":"2004","journal-title":"Endocr. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1677\/joe.1.06269","article-title":"Oxidative and nitrosative stress in brain mitochondria of diabetic rats","volume":"187","author":"Mastrocola","year":"2005","journal-title":"J. Endocrinol."},{"key":"ref_7","first-page":"213","article-title":"Type 2 Diabetes Mellitus and Cognitive Impairment","volume":"53","author":"Damanik","year":"2021","journal-title":"Acta Med. Indones."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3242","DOI":"10.1111\/os.13542","article-title":"The Effect of Diabetes Mellitus on the Neurological Function of Patients with Cervical Spondylotic Myelopathy","volume":"14","author":"Jiang","year":"2022","journal-title":"Orthop. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3748\/wjg.v13.i2.175","article-title":"Early diabetic neuropathy: Triggers and mechanisms","volume":"13","author":"Dobretsov","year":"2007","journal-title":"World J. Gastroenterol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.neulet.2012.10.031","article-title":"The size of motoneurons of the gastrocnemius muscle in rats with diabetes","volume":"531","author":"Muramatsu","year":"2012","journal-title":"Neurosci. Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/0014-4886(79)90134-1","article-title":"Distribution of intravenously injected protein tracers in peripheral ganglia of adult mice","volume":"63","author":"Arvidson","year":"1979","journal-title":"Exp. Neurol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.nbd.2006.11.016","article-title":"Does diabetes mellitus target motor neurons?","volume":"26","author":"Ramji","year":"2007","journal-title":"Neurobiol. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.neulet.2008.04.032","article-title":"Diabetes affects the expression of GABA and potassium chloride cotransporter in the spinal cord: A study in streptozotocin diabetic rats","volume":"438","author":"Morgado","year":"2008","journal-title":"Neurosci. Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1002\/dmrr.359","article-title":"Neuropathic pain and diabetes","volume":"19","author":"Kapur","year":"2003","journal-title":"Diabetes Metab. Res. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1002\/dmrr.1225","article-title":"Painful diabetic peripheral neuropathy: Consensus recommendations on diagnosis, assessment and management","volume":"27","author":"Tesfaye","year":"2011","journal-title":"Diabetes Metab. Res. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3675","DOI":"10.1113\/JP275067","article-title":"Diabetes-induced microvascular complications at the level of the spinal cord: A contributing factor in diabetic neuropathic pain","volume":"596","author":"Ved","year":"2018","journal-title":"J. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2318","DOI":"10.1152\/jn.00423.2017","article-title":"alpha-Motoneurons maintain biophysical heterogeneity in obesity and diabetes in Zucker rats","volume":"118","author":"MacDonell","year":"2017","journal-title":"J. Neurophysiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1007\/s11845-026-04355-6","article-title":"Cervical spinal cord enlargement and systemic inflammation in diabetic peripheral neuropathy: A cross-sectional MRI study","volume":"195","author":"Atik","year":"2026","journal-title":"Ir. J. Med. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"9940169","DOI":"10.1155\/2021\/9940169","article-title":"Therapeutic Potential of Polyphenols in the Management of Diabetic Neuropathy","volume":"2021","author":"Kabir","year":"2021","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1139\/apnm-2015-0145","article-title":"Effects of curcumin and captopril on the functions of kidney and nerve in streptozotocin-induced diabetic rats: Role of angiotensin converting enzyme 1","volume":"40","author":"Gomaa","year":"2015","journal-title":"Appl. Physiol. Nutr. Metab."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.neulet.2013.12.019","article-title":"Curcumin ameliorated diabetic neuropathy partially by inhibition of NADPH oxidase mediating oxidative stress in the spinal cord","volume":"560","author":"Zhao","year":"2014","journal-title":"Neurosci. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1021\/nn405077y","article-title":"Curcumin-loaded nanoparticles potently induce adult neurogenesis and reverse cognitive deficits in Alzheimer\u2019s disease model via canonical Wnt\/beta-catenin pathway","volume":"8","author":"Tiwari","year":"2014","journal-title":"ACS Nano"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1742-2094-11-59","article-title":"Curcumin attenuates acute inflammatory injury by inhibiting the TLR4\/MyD88\/NF-kappaB signaling pathway in experimental traumatic brain injury","volume":"11","author":"Zhu","year":"2014","journal-title":"J. Neuroinflamm."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"263","DOI":"10.3233\/JAD-2010-091528","article-title":"Severe motor neuron degeneration in the spinal cord of the Tg2576 mouse model of Alzheimer disease","volume":"21","author":"Seo","year":"2010","journal-title":"J. Alzheimers Dis."},{"key":"ref_25","first-page":"8708961","article-title":"An Eluate of the Medicinal Plant Garcinia kola Displays Strong Antidiabetic and Neuroprotective Properties in Streptozotocin-Induced Diabetic Mice","volume":"2022","author":"Hamza","year":"2022","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"20160167","DOI":"10.1515\/jcim-2016-0167","article-title":"Garcinia kola seeds may prevent cognitive and motor dysfunctions in a type 1 diabetes mellitus rat model partly by mitigating neuroinflammation","volume":"14","author":"Farahna","year":"2017","journal-title":"J. Complement. Integr. Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sohn, S.I., Priya, A., Balasubramaniam, B., Muthuramalingam, P., Sivasankar, C., Selvaraj, A., Valliammai, A., Jothi, R., and Pandian, S. (2021). Biomedical applications and bioavailability of curcumin-an updated overview. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13122102"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1007\/s11101-023-09869-w","article-title":"Garcinia kola: A critical review on chemistry and pharmacology of an important West African medicinal plant","volume":"22","author":"Tauchen","year":"2023","journal-title":"Phytochem. Rev."},{"key":"ref_29","first-page":"55","article-title":"Bilateral elevation of interleukin-6 protein and mRNA in both lumbar and cervical dorsal root ganglia following unilateral chronic compression injury of the sciatic nerve","volume":"10","year":"2013","journal-title":"J. Neuroinflamm."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1007\/s12031-021-01958-9","article-title":"Plasticity in Motoneurons Following Spinal Cord Injury in Fructose-induced Diabetic Rats","volume":"72","author":"Simonyan","year":"2022","journal-title":"J. Mol. Neurosci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/B978-0-444-53480-4.00030-8","article-title":"Experimental motor neuropathy in diabetes","volume":"Volume 126","author":"Wilson","year":"2014","journal-title":"Handbook of Clinical Neurology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.brainres.2004.05.073","article-title":"Reduction of the spinal nucleus of the bulbocavernosous volume by experimental diabetes","volume":"1019","author":"Dorfman","year":"2004","journal-title":"Brain Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/sj.cdd.4400476","article-title":"Emerging roles of caspase-3 in apoptosis","volume":"6","author":"Porter","year":"1999","journal-title":"Cell Death Differ."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1073\/pnas.0232966100","article-title":"Caspase 3 activation is essential for neuroprotection in preconditioning","volume":"100","author":"McLaughlin","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.autneu.2009.12.003","article-title":"Effects of diabetes on expression of glial fibrillary acidic protein and neurotrophins in rat colon","volume":"154","author":"Liu","year":"2010","journal-title":"Auton. Neurosci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4053","DOI":"10.1096\/fj.201700110R","article-title":"Glial fibrillary acidic protein (GFAP) is a novel biomarker for the prediction of autoimmune diabetes","volume":"31","author":"Pang","year":"2017","journal-title":"FASEB J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"148519","DOI":"10.1016\/j.brainres.2023.148519","article-title":"Curcumin modulates astrocyte function under basal and inflammatory conditions","volume":"1818","author":"Seady","year":"2023","journal-title":"Brain Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"164","DOI":"10.2174\/187152411796011303","article-title":"Astrocytes: Targets for neuroprotection in stroke","volume":"11","author":"Barreto","year":"2011","journal-title":"Cent. Nerv. Syst. Agents Med. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"a020420","DOI":"10.1101\/cshperspect.a020420","article-title":"Astrogliosis","volume":"7","author":"Sofroniew","year":"2014","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1002\/glia.20068","article-title":"Effects of diabetes mellitus on astrocyte GFAP and glutamate transporters in the CNS","volume":"48","author":"Coleman","year":"2004","journal-title":"Glia"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e2870","DOI":"10.1002\/brb3.2870","article-title":"Oxidative stress disrupts the cytoskeleton of spinal motor neurons","volume":"13","author":"Chen","year":"2023","journal-title":"Brain Behav."},{"key":"ref_42","first-page":"356","article-title":"The Streptozotocin-Induced Diabetic Nude Mouse Model: Differences between Animals from Different Sources","volume":"61","author":"Graham","year":"2011","journal-title":"Comp. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1002\/1097-0010(200011)80:14<2021::AID-JSFA745>3.0.CO;2-2","article-title":"Evaluation of antioxidant activities of rosehip ethanol extracts in different test system","volume":"80","author":"Gao","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.jep.2016.11.001","article-title":"Garcinia kola aqueous suspension prevents cerebellar neurodegeneration in long-term diabetic rat\u2014A type 1 diabetes mellitus model","volume":"195","author":"Farahna","year":"2017","journal-title":"J. Ethnopharmacol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.scienta.2008.02.003","article-title":"Physico-chemical and antioxidant properties of cornelian cherry fruits (Cornus mas L.) grown in Turkey","volume":"116","author":"Tural","year":"2008","journal-title":"Sci. Hortic."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"035025","DOI":"10.1088\/1748-605X\/adcfe3","article-title":"Neuroprotective effects of Garcinia kola and curcumin on diabetic transected sciatic nerve","volume":"20","author":"Hamour","year":"2025","journal-title":"Biomed. Mater."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/8\/1301\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:38:49Z","timestamp":1786984729000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/8\/1301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":47,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ph19081301"],"URL":"https:\/\/doi.org\/10.3390\/ph19081301","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}