{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:30:30Z","timestamp":1787585430658,"version":"build-2736575974"},"reference-count":41,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>This exploratory study compared the flowers, leaves, and roots of Hypochaeris sessiliflora to evaluate physicochemical properties, bioactive compounds, in vitro chemical antioxidant activity, antimicrobial activity, and locomotion-disrupting effects on Caenorhabditis elegans. Bioactive compounds were determined by liquid chromatography; in vitro chemical antioxidant activity by microplate spectrophotometry; antimicrobial activity against a range of ATCC and multidrug-resistant microorganisms; and locomotion-disrupting effects on Caenorhabditis elegans. The results revealed significant differences between plant organs. The leaves contained the highest concentrations of calcium (1163.1 mg\/100 g DW), potassium (3256.6 mg\/100 g DW), vitamin C (39.2 mg\/100 g DW), organic acids (1213.5 mg\/100 g DW), and total quantified chlorophylls (89.0 mg\/100 g DW). The flowers had the highest levels of total quantified carotenoids (152.1 mg\/100 g DM) and total quantified phenolic compounds (12,341.1 mg\/100 g DM). They exhibited the highest in vitro chemical antioxidant activity, as measured by the ABTS assay (6.1 mmol TE\/100 g DW). The extracts exhibited antimicrobial activity that depended on the plant organ and the microorganism tested, with the root extract being the most active against Staphylococcus epidermidis (MIC = 8.2 mg\/mL). No activity was detected against Candida species or against multidrug-resistant bacteria under the experimental conditions used. Furthermore, the extracts under the experimental conditions used did not significantly alter C. elegans\u2019 locomotion. Overall, this study provides baseline evidence of the organ-specific chemical composition and biological properties of H. sessiliflora, supporting further investigation of the compounds responsible for the observed in vitro chemical antioxidant and selective and antimicrobial activities.<\/jats:p>","DOI":"10.3390\/ph19081314","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T12:57:53Z","timestamp":1787230673000},"page":"1314","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unexplored Bioactive, Antioxidant, Antimicrobial, and Nematicidal Potential of the Flowers, Leaves, and Roots of \u2018Yellow Chicory\u2019 (Hypochaeris sessiliflora)"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4495-3500","authenticated-orcid":false,"given":"Elena","family":"Coyago-Cruz","sequence":"first","affiliation":[{"name":"Carrera de Ingenier\u00eda en Biotecnolog\u00eda, Universidad Polit\u00e9cnica Salesiana, Sede Quito, Campus El Gir\u00f3n, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5903-6735","authenticated-orcid":false,"given":"Gabriela","family":"M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Carrera de Ingenier\u00eda en Biotecnolog\u00eda, Universidad Polit\u00e9cnica Salesiana, Sede Quito, Campus El Gir\u00f3n, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9037-9306","authenticated-orcid":false,"given":"Johana","family":"Z\u00fa\u00f1iga-Miranda","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica (CENBIO), Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0368-4328","authenticated-orcid":false,"given":"Alejandro","family":"Porras","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Quito 171103, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8129-233X","authenticated-orcid":false,"given":"Carlos","family":"Barba-Ostria","sequence":"additional","affiliation":[{"name":"Escuela de Medicina, Colegio de Ciencias de la Salud Quito, Universidad San Francisco de Quito (USFQ), Quito 170901, Ecuador"},{"name":"Instituto de Microbiolog\u00eda, Universidad San Francisco de Quito (USFQ), Quito 170901, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivanna","family":"Sarabia","sequence":"additional","affiliation":[{"name":"Carrera de Ingenier\u00eda en Biotecnolog\u00eda, Universidad Polit\u00e9cnica Salesiana, Sede Quito, Campus El Gir\u00f3n, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3756-2387","authenticated-orcid":false,"given":"Linda","family":"Guam\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica (CENBIO), Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3393-5522","authenticated-orcid":false,"given":"Celina","family":"Coello","sequence":"additional","affiliation":[{"name":"Carrera de Ingenier\u00eda en Biotecnolog\u00eda, Universidad Polit\u00e9cnica Salesiana, Sede Quito, Campus El Gir\u00f3n, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7620-9140","authenticated-orcid":false,"given":"Jorge","family":"Heredia-Moya","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica (CENBIO), Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0401-873X","authenticated-orcid":false,"given":"Blanca","family":"Naranjo","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Quito 171103, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7597-2977","authenticated-orcid":false,"given":"Mar\u00eda","family":"Segovia-Salcedo","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Quito 171103, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1016\/j.biotechadv.2015.08.001","article-title":"Discovery and Resupply of Pharmacologically Active Plant-Derived Natural Products: A Review","volume":"33","author":"Atanassov","year":"2015","journal-title":"Biotechnol. Adv."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nrd4510","article-title":"The Re-Emergence of Natural Products for Drug Discovery in the Genomics Era","volume":"14","author":"Harvey","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Pi\u0105tkowska, E., Biel, W., Witkowicz, R., and K\u0119pi\u0144ska-Pacelik, J. (2022). Chemical Composition and Antioxidant Activity of Asteraceae Family Plants. Appl. Sci., 12.","DOI":"10.3390\/app122312293"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Kozuharova, E., Malfa, G., Acquaviva, R., Spadaro, V., Ionkova, I., Statti, G., and Raimondo, F. (2026). The Wild Plants from the Family Asteraceae That Are Traditionally Used for Food in Sicily and Bulgaria and Their Health Benefits. Foods, 15.","DOI":"10.3390\/foods15060988"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhu, H., Liu, C., and Qian, H. (2022). Pharmaceutical Potential of High-Altitude Plants for Fatigue-Related Disorders: A Review. Plants, 11.","DOI":"10.3390\/plants11152004"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Souilah, N., Ullah, Z., Bendif, H., Medjroubi, K., Hazmoune, T., Hamel, T., \u00d6zt\u00fcrk, M., Nieto, G., and Akkal, S. (2020). Phenolic Compounds from an Algerian Endemic Species of Hypochaeris laevigata Var. hipponensis and Investigation of Antioxidant Activities. Plants, 9.","DOI":"10.3390\/plants9040514"},{"key":"ref_7","unstructured":"(2024, June 14). WFO The World Flora Online. Available online: https:\/\/wfoplantlist.org\/plant-list."},{"key":"ref_8","unstructured":"(2026, June 26). MBG Hypochaeris Sessiliflora Kunth. Available online: https:\/\/www.mobot.org\/mobot\/ParamoCajas\/results.aspx?taxname=Hypochaeris%20sessiliflora."},{"key":"ref_9","unstructured":"(2026, June 26). WHO Antimicrobial Resistance. Available online: http:\/\/www.who.int\/en\/news-room\/fact-sheets\/detail\/antimicrobial-resistance."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"M\u00e9ndez, G., Coyago-Cruz, E., Lomas, P., Cerna, M., and Heredia-Moya, J. (2025). Functional, Antioxidant, and Antimicrobial Profile of Medicinal Leaves from the Amazon. Antioxidants, 14.","DOI":"10.3390\/antiox14080965"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Coyago-Cruz, E., M\u00e9ndez, G., Z\u00fa\u00f1iga-Miranda, J., Jami, N., Acurio-V\u00e1sconez, R., and Heredia-Moya, J. (2025). Dynamics of Bioactive Compounds and Their Relationship with Antioxidant and Antimicrobial Activity in the Pulp, Peel, and Seeds of \u2018 Salak\u2019 during Ripening. Foods, 14.","DOI":"10.3390\/foods14203476"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Coyago-Cruz, E., Mayorga-Ramos, A., M\u00e9ndez, G., Alpusig-Guanoluisa, L., Rivera-Rueda, F., Z\u00fa\u00f1iga-Miranda, J., Barba-Ostria, C., and Heredia-Moya, J. (2026). Functional Evaluation, Antioxidant, Antimicrobial, Antibiofilm, and Haemolytic Capacity of Calathea lutea (Bijao) and Calathea inocephala (Shutupipanga) Leaves. Antioxidants, 15.","DOI":"10.3390\/antiox15030274"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Coyago-Cruz, E., Barrigas, A., Guachamin, A., Heredia-Moya, J., Zu\u00f1iga-Miranda, J., and Vera, E. (2024). Bioactive Composition of Tropical Flowers and Their Antioxidant and Antimicrobial Properties. Foods, 13.","DOI":"10.3390\/foods13233766"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"23407","DOI":"10.1038\/s41598-024-74068-2","article-title":"Standardisation of High Throughput Microdilution Antifungal Susceptibility Testing for Candida albicans and Cryptococcus neoformans","volume":"14","author":"Floyd","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_15","unstructured":"(2024). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically (Standard No. CLSI M07). Available online: https:\/\/clsi.org\/shop\/standards\/m07\/."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.jgg.2023.10.004","article-title":"Two Diversities Meet in the Rhizosphere: Root Specialized Metabolites and Microbiome","volume":"51","author":"Wang","year":"2024","journal-title":"J. Genet. Genom."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zhang, C., Feng, C., Zheng, Y., Wang, J., and Wang, F. (2020). Root Exudates Metabolic Profiling Suggests Distinct Defense Mechanisms between Resistant and Susceptible Tobacco Cultivars Aagainst Black Shank Disease. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.559775"},{"key":"ref_18","first-page":"21","article-title":"Physicochemical Characteristics and Antioxidant Capacity of Ecuadorian Paramo Flowers","volume":"8","author":"Guachamin","year":"2023","journal-title":"Rev. Bionatura"},{"key":"ref_19","first-page":"123","article-title":"Caracterizaci\u00f3n de Plantas Medicinales Nativas de Yacubiana e Identificaci\u00f3n de Compuestos Bioactivos Para El Control Biol\u00f3gico","volume":"12","author":"Bonilla","year":"2025","journal-title":"UTCiencia Cienc. Tecnol. Serv. Pueblo"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"685","DOI":"10.3417\/2006136","article-title":"Systematics of the South American Hypochaeris Sessiliflora Complex (Asteraceae, Cichorieae)","volume":"96","author":"Urtubey","year":"2009","journal-title":"Ann. Mo. Bot. Gard."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Vojvodi\u0107, S., Bo\u017eovi\u0107, D., A\u0107imovi\u0107, M., Ga\u0161i\u0107, U., Zekovi\u0107, Z., Bebek, A., Bursa\u0107, D., Zlatkovi\u0107, B., and Pavli\u0107, B. (2025). A Preliminary Insight into Under-Researched Plants from the Asteraceae Family in the Balkan Peninsula: Bioactive Compound Diversity and Antioxidant Potential. Plants, 14.","DOI":"10.3390\/plants14182904"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"34753","DOI":"10.1038\/s41598-025-18323-0","article-title":"Phytochemical Profiling, HPLC Analysis, and Antimicrobial Potential of Curio radicans (L. f.) P.V. Heath","volume":"15","author":"Naseer","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1126\/science.aaz7614","article-title":"The Physiology of Plant Responses to Drought","volume":"368","author":"Gupta","year":"2020","journal-title":"Science"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Overman, A.R., and Scholtz, R.V. (2013). Accumulation of Biomass and Mineral Elements with Calendar Time by Cotton: Application of the Expanded Growth Model. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0072810"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ciocarlan, A., Shvetsova, M., Zinicovscaia, I., Chaligava, O., Grozdov, D., Aricu, A., and Ciocarlan, N. (2024). Mineral Composition of Fifteen Species of Asteraceae Family Growing in the Republic of Moldova Using Neutron Activation Analysis. Agronomy, 14.","DOI":"10.3390\/agronomy14102387"},{"key":"ref_26","first-page":"45","article-title":"Comparative Analysis of Ascorbic and Phenol Content Percentage in Leaves, Stems and Flowers of Hibiscus rosa Sinensis","volume":"9","author":"Tyagi","year":"2017","journal-title":"Der Pharm. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Tanasa, M.V., Negreanu-Pirjol, T., Olariu, L., Negreanu-Pirjol, B., Lepadatu, A., Anghel, L., and Rosoiu, N. (2025). Bioactive Compounds from Vegetal Organs of Taraxacum Species (Dandelion) with Biomedical Applications: A Review. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26020450"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Coyago-Cruz, E., Moya, M., M\u00e9ndez, G., Villac\u00eds, M., Rojas-Silva, P., Corell, M., Mapelli-Brahm, P., Vicario, I., and Mel\u00e9ndez-Mart\u00ednez, A. (2023). Exploring Plants with Flowers: From Therapeutic Nutritional Benefits to Innovative Sustainable Uses. Foods, 12.","DOI":"10.3390\/foods12224066"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Sicari, V., Loizzo, M., Silva, A., Romeo, R., Spampinato, G., Tundis, R., Leporini, M., and Musarella, C. (2021). The Effect of Blanching on Phytochemical Content and Bioactivity of Hypochaeris and Hyoseris Species (Asteraceae), Vegetables Traditionally Used in Southern Italy. Foods, 10.","DOI":"10.3390\/foods10010032"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Platzer, M., Kiese, S., Herfellner, T., Schweiggert-Weisz, U., Miesbauer, O., and Eisner, P. (2021). Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays. Molecules, 26.","DOI":"10.3390\/molecules26051244"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Rolnik, A., and Olas, B. (2021). The Plants of the Asteraceae Family as Agents in the Protection of Human Health. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22063009"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Randriatsalama, A., Ranarivelo, N., Razafintsalama, V., and Ranaivoarisoa, R. (2024). Antimicrobial Properties of Selected Plant Species within the Asteraceae Family. Biol. Life Sci. Forum, 31.","DOI":"10.3390\/ECM2023-16410"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"153626","DOI":"10.1016\/j.phymed.2021.153626","article-title":"Antibacterial Plant Compounds, Extracts and Essential Oils: An Updated Review on Their Effects and Putative Mechanisms of Action","volume":"90","author":"Micol","year":"2021","journal-title":"Phytomedicine"},{"key":"ref_34","first-page":"758","article-title":"In Vitro Antifungal Activity of Leaf and Root Extracts of the Medicinal Plant, Hypochaeris radicata L.","volume":"5","author":"Senguttuvan","year":"2013","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Teodoro, G., Ellepola, K., Seneviratne, C., and Koga-Ito, C. (2015). Potential Use of Phenolic Acids as Anti-Candida Agents: A Review. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.01420"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"17903","DOI":"10.3390\/molecules201017903","article-title":"Activity of Polyphenolic Compounds against Candida glabrata","volume":"20","year":"2015","journal-title":"Molecules"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.1128\/AAC.50.5.1710-1714.2006","article-title":"Antifungal Activity of C-27 Steroidal Saponins","volume":"50","author":"Yang","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"107274","DOI":"10.1016\/j.actatropica.2024.107274","article-title":"Seasonal and Circadian Evaluation of Ageratum Conyzoides Essential Oil and Its Nematicidal Activity against Caenorhabditis elegans","volume":"256","author":"Lima","year":"2024","journal-title":"Acta Trop."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3723567","DOI":"10.1155\/2022\/3723567","article-title":"Ferulic Acid Exerts Neuroprotective Effects via Autophagy Induction in C. elegans and Cellular Models of Parkinson\u2019s Disease","volume":"2022","author":"Long","year":"2022","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/s13071-015-0708-6","article-title":"Nematicidal Activity of Annona crassiflora Leaf Extract on Caenorhabditis elegans","volume":"8","author":"Machado","year":"2015","journal-title":"Parasites Vectors"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e14456","DOI":"10.1111\/jfbc.14456","article-title":"Responses of Bitter Melon Saponins to Oxidative Stress and Aging via the IIS Pathway Linked with Sir-2.1 and Hlh-30","volume":"46","author":"Zhang","year":"2022","journal-title":"J. Food Biochem."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/8\/1314\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T14:57:57Z","timestamp":1787583477000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/8\/1314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,20]]},"references-count":41,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ph19081314"],"URL":"https:\/\/doi.org\/10.3390\/ph19081314","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,20]]}}}