{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:32:59Z","timestamp":1787567579992,"version":"build-2736575974"},"reference-count":25,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangxi Science and Technology Major Program","award":["GuiKe AA22096022"],"award-info":[{"award-number":["GuiKe AA22096022"]}]},{"name":"Guangxi Peak Discipline: Traditional Chinese Medicine","award":["2 [2026]"],"award-info":[{"award-number":["2 [2026]"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Alzheimer\u2019s disease (AD) is a prevalent neurodegenerative disorder for which effective treatments remain limited. Liubao tea, a distinctive post-fermented dark tea, develops unique aged and areca nut aromas through prolonged storage. However, comprehensive studies on its anti-Alzheimer\u2019s disease effects are lacking. This study aimed to identify the bioactive constituents of Liubao tea with varying storage durations and aroma profiles, and to further evaluate its neuroprotective potential against Alzheimer\u2019s disease. Methods: Chromatography and spectrophotometry were employed to quantitatively determine the primary bioactive components in various Liubao tea samples. A D-galactose-induced Alzheimer\u2019s disease zebrafish (Danio rerio) model was established to evaluate the neuroprotective effects of the aqueous extract of Liubao tea. Results: The contents of theabrownins, theaflavins, tea pigments, and catechins showed potential associations with the anti-AD capacity of Liubao tea. Variations in these bioactive components appeared to correlate with the neuroprotective activity. Conclusions: This study elucidates the anti-AD potential of aged Liubao tea and associates its characteristic constituents with neuroprotective effects. The findings provide essential theoretical support for developing Liubao tea as a promising natural product candidate worthy of further anti-AD research.<\/jats:p>","DOI":"10.3390\/ph19091336","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:20:02Z","timestamp":1787563202000},"page":"1336","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Potential Ameliorative Effect of Aged Liubao Tea Aqueous Extract on D-Galactose-Induced Pathological Damage in an Alzheimer\u2019s Disease Zebrafish Model"],"prefix":"10.3390","volume":"19","author":[{"given":"Shini","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Li","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, China"},{"name":"Teaching and Experimental Training Centre, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hangxing","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganghua","family":"Huang","sequence":"additional","affiliation":[{"name":"China Tea (Wuzhou) Co., Ltd., Wuzhou 543000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Leng","sequence":"additional","affiliation":[{"name":"Guangxi Key Lab High Incidence Infect Dis Integrat, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"155834","DOI":"10.1016\/j.phymed.2024.155834","article-title":"Integrated Bioinformatics and Multiomics Reveal Liupao Tea Extract Alleviating NAFLD via Regulating Hepatic Lipid Metabolism and Gut Microbiota","volume":"132","author":"Yan","year":"2024","journal-title":"Phytomedicine"},{"key":"ref_2","first-page":"118776","article-title":"Neuroprotective Effects of Polyphenol-Rich Extracts Obtained from Agricultural By-Products in an Induced Cognitive Decline Model of Zebrafish Larvae and in Human Neurons","volume":"188","author":"Tchoumtchoua","year":"2025","journal-title":"Biomed. 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