{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T09:07:12Z","timestamp":1789031232729,"version":"build-2803163510"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation","award":["DTCRD-113(2)-E-18"],"award-info":[{"award-number":["DTCRD-113(2)-E-18"]}]},{"award":["DTCRD-113(2)-E-18"],"award-info":[{"award-number":["DTCRD-113(2)-E-18"]}],"id":[{"id":"https:\/\/ror.org\/04rbvc675","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Chung Shan Medical University and Changhua Christian Hospital","award":["CSMU-CCH-113-10"],"award-info":[{"award-number":["CSMU-CCH-113-10"]}]},{"award":["CSMU-CCH-113-10"],"award-info":[{"award-number":["CSMU-CCH-113-10"]}],"id":[{"id":"https:\/\/ror.org\/05d9dtr71","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Interstitial cystitis\/bladder pain syndrome (IC\/BPS) is a chronic inflammatory bladder disorder that lacks an effective treatment agent. Fucoidan (FUC), isolated from Fucus vesiculosus, is a sulfated polysaccharide that exhibits potent anti-inflammatory and anti-fibrotic activities. This study investigated the protective effects and underlying mechanisms of FUC in IC\/BPS. Methods: NLRP3 inflammasome activation and AKT signaling were evaluated by Western blotting, qRT-PCR, immunofluorescence, pharmacological inhibition, gene silencing, and overexpression. Molecular docking assessed potential FUC\u2013protein interactions. In vivo mouse model evaluated the protective effects and toxicity of FUC. Results: In SV-HUC-1 cells, FUC significantly attenuated TNF-\u03b1-induced expression of NLRP3 and cleaved caspase-1 and c-IL-1\u03b2 expression at both the transcriptional and protein levels. Residual inflammasome activity after FUC treatment remained further suppressible by MCC950 or siNLRP3. FUC also reduced TNF\u03b1-induced AKT phosphorylation, whereas AKT silencing and overexpression enhanced and partially reversed the inhibitory effect of FUC on inflammasome signaling, respectively. The molecular docking results suggested potential interactions between FUC and key inflammasome-related proteins, including NLRP3, caspase-1, IL-1\u03b2, and AKT. In interstitial cystitis mice, FUC mitigated cyclophosphamide (CYP)-induced bladder injury, reduced bladder expression of inflammasome-associated markers, and exhibited no significant toxicity in major organs. Conclusions: These findings indicate that FUC alleviates urothelial inflammatory injury and experimental cystitis, at least in part, by suppressing the AKT\/NLRP3 inflammasome axis, supporting its potential as a therapeutic candidate for IC\/BPS.<\/jats:p>","DOI":"10.3390\/ph19091427","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:07:41Z","timestamp":1789027661000},"page":"1427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fucoidan from Fucus Vesiculosus Alleviates Interstitial Cystitis by Suppressing the AKT\/NLRP3 Inflammasome Axis"],"prefix":"10.3390","volume":"19","author":[{"given":"Chun-Shuo","family":"Hsu","sequence":"first","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"},{"name":"Department of Obstetrics and Gynecology, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi 62247, Taiwan"},{"name":"School of Medicine, Tzu Chi University, Hualien 970374, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Chu-Liang","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5123-4729","authenticated-orcid":false,"given":"Yong-Syuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Chung Shan Medical University Hospital, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Hung-Ju","family":"Chien","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua 50006, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4942-1888","authenticated-orcid":false,"given":"Yi-Hsien","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"},{"name":"Department of Medical Research, Chung Shan Medical University Hospital, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"865463","DOI":"10.1155\/2014\/865463","article-title":"Evidence for bladder urothelial pathophysiology in functional bladder disorders","volume":"2014","author":"Keay","year":"2014","journal-title":"BioMed Res. 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