{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T11:16:37Z","timestamp":1789125397008,"version":"build-2803163510"},"reference-count":69,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sirius Federal Territory","award":["25-03"],"award-info":[{"award-number":["25-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Microalgae and cyanobacteria represent a largely unexplored source of bioactive compounds, but systematic screening of extensive strain collections is limited by the cost and complexity of conventional chemical and pharmacological characterization. Here, we evaluated a scalable functional screening strategy integrating human cell-based gene expression profiling, quantitative molecular pathway analysis, and direct cytotoxicity testing to prioritize bioactive microalgal and cyanobacterial extracts for subsequent investigation. Results: Forty-eight extracts were screened using HL-60 human leukemia cells, and eight demonstrated detectable cytotoxic activity. Pathway-based prediction showed 92% agreement with direct cytotoxicity measurements. To assess whether pathway profiling could provide information on mechanisms of action, we performed an in-depth analysis of the most active Nostoc sp. SBV-48 extract and the control antimicrobial peptide Polyphemusin III. The inferred pathway responses were consistent with their previously reported mechanisms of cytotoxicity. Fractionation and chemical characterization of the Nostoc sp. SBV-48 extract identified Cryptophycin-1 as its major cytotoxic component, illustrating the intended sequential workflow from primary screening to characterization of prioritized hits. In addition, previously unreported cytotoxic activity against HL-60 cells was detected in three Desmidiales strains belonging to the genera Closterium, Cosmarium, and Spondylosium, which showed partially convergent pathway-level responses. Conclusions: Overall, the proposed approach provides a cost-effective first-stage strategy for functional screening and prioritization of complex microalgal extracts, allowing resource-intensive chemical characterization and broader pharmacological validation to be focused on the most promising candidates.<\/jats:p>","DOI":"10.3390\/ph19091440","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T10:51:37Z","timestamp":1789123897000},"page":"1440","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Cell-Based Transcriptomic and Pathway Profiling Enables Scalable Functional Screening of Bioactive Microalgal Extracts"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2773-3486","authenticated-orcid":false,"given":"Boris","family":"Sorokin","sequence":"first","affiliation":[{"name":"Moscow Center for Advanced Studies, 123592 Moscow, 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Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Daniil","family":"Luppov","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 123592 Moscow, Russia"},{"name":"Research Center for Genetics and Life Sciences, Sirius University of Science and Technology, 354340 Sirius, Russia"},{"name":"Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia, 123098 Moscow, 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J."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Olasehinde, T.A., Olaniran, A.O., and Okoh, A.I. (2017). Therapeutic potentials of microalgae in the treatment of Alzheimer\u2019s disease. Molecules, 22.","DOI":"10.3390\/molecules22030480"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.4194\/1303-2712-v18_10_06","article-title":"Influence of media and temperature on the growth and biological activities of Desmodesmus protuberans (F.E. Fritsch & M.F. Rich) E. Hegewald","volume":"18","author":"Demirel","year":"2018","journal-title":"Turk. J. Fish. Aquat. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"G\u00fcrlek, C., Yarkent, C., K\u00f6se, A., Oral, I., \u00d6ncel, S.S., and Elibol, M. (2019). Evaluation of several microalgal extracts as bioactive metabolites as potential pharmaceutical compounds. CMBEBIH 2019, Springer Nature.","DOI":"10.1007\/978-3-030-17971-7_41"},{"key":"ref_5","first-page":"100203","article-title":"Microalgae contribution in enhancing the circular economy drive of biochemical conversion systems\u2013A review","volume":"7","author":"Anyaoha","year":"2024","journal-title":"Carbon Resour. Convers."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.nbt.2020.11.011","article-title":"The role of microalgae in the bioeconomy","volume":"61","author":"Reis","year":"2021","journal-title":"New Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"43","DOI":"10.22159\/ajpcr.2016.v9i6.14507","article-title":"Pharmaceutically valuable bioactive compounds of algae","volume":"7","author":"Bhattacharjee","year":"2016","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/jpy.13431","article-title":"How many species of algae are there? A reprise. Four kingdoms, 14 phyla, 63 classes and still growing","volume":"60","author":"Guiry","year":"2024","journal-title":"J. Phycol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Dolganyuk, V., Belova, D., Babich, O., Prosekov, A., Ivanova, S., Katserov, D., Patyukov, N., and Sukhikh, S. (2020). Microalgae: A promising source of valuable bioproducts. Biomolecules, 10.","DOI":"10.3390\/biom10081153"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"150585","DOI":"10.1016\/j.scitotenv.2021.150585","article-title":"Algae biotechnology for industrial wastewater treatment, bioenergy production, and high-value bioproducts","volume":"806","author":"Ahmad","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Fabris, M., Abbriano, R.M., Pernice, M., Sutherland, D.L., Commault, A.S., Hall, C.C., Labeeuw, L., McCauley, J.I., Kuzhiuparambil, U., and Ray, P. (2020). Emerging technologies in algal biotechnology: Toward the establishment of a sustainable, algae-based bioeconomy. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.00279"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3823","DOI":"10.1002\/jsfa.13136","article-title":"Current challenges of microalgae applications: Exploiting the potential of non-conventional microalgae species","volume":"104","author":"Occhipinti","year":"2024","journal-title":"J. Sci. Food Agric."},{"key":"ref_13","first-page":"367","article-title":"Algal biotechnology","volume":"Volume 26","author":"Sahoo","year":"2015","journal-title":"The Algae World"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Abomohra, A., and Ende, S. (2024). Omics approaches for algal applications. Value-Added Products from Algae, Springer.","DOI":"10.1007\/978-3-031-42026-9"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1007\/s11101-022-09804-5","article-title":"Recent breakthroughs in integrated biomolecular and biotechnological approaches for enhanced lipid and carotenoid production from microalgae","volume":"22","author":"Ahmad","year":"2023","journal-title":"Phytochem. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1186\/s12934-021-01656-6","article-title":"Algal glycobiotechnology: Omics approaches for strain improvement","volume":"20","author":"Sirohi","year":"2021","journal-title":"Microb. Cell Fact."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Lauritano, C., Ferrante, M.I., and Rogato, A. (2019). Marine natural products from microalgae: An-omics overview. Mar. Drugs, 17.","DOI":"10.3390\/md17050269"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1089\/omi.2016.0065","article-title":"Multi-omics frontiers in algal research: Techniques and progress to explore biofuels in the postgenomics world","volume":"20","author":"Rai","year":"2016","journal-title":"OMICS J. Integr. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wolf, J., Chapman, R., Deepika, C., Pietri, M., Bensalem, S., and Hankamer, B. (2023). High-throughput screening to accelerate microalgae-based phycochemical production. Value-Added Products from Algae: Phycochemical Production and Applications, Springer International Publishing.","DOI":"10.1007\/978-3-031-42026-9_10"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Tripathi, B.N., and Kumar, D. (2017). Prospects and Challenges in Algal Biotechnology, Springer.","DOI":"10.1007\/978-981-10-1950-0"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.algal.2017.03.016","article-title":"Fucoxanthin production by heterokont microalgae","volume":"24","author":"Petrushkina","year":"2017","journal-title":"Algal Res."},{"key":"ref_22","first-page":"1","article-title":"Using proteomic and transcriptomic data to assess activation of intracellular molecular pathways","volume":"127","author":"Buzdin","year":"2021","journal-title":"Adv. Protein Chem. Struct. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"109716","DOI":"10.1016\/j.compbiomed.2025.109716","article-title":"Prospective trial of the Oncobox platform RNA sequencing bioinformatic analysis for personalized prescription of targeted drugs","volume":"187","author":"Sorokin","year":"2025","journal-title":"Comput. Biol. Med."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sorokin, M., Borisov, N., Kuzmin, D., Gudkov, A., Zolotovskaia, M., Garazha, A., and Buzdin, A. (2021). Algorithmic annotation of functional roles for components of 3,044 human molecular pathways. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.617059"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2280","DOI":"10.1016\/j.csbj.2022.05.006","article-title":"OncoboxPD: Human 51 672 molecular pathways database with tools for activity calculating and visualization","volume":"20","author":"Zolotovskaia","year":"2022","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Marggraf, M.B., Panteleev, P.V., Emelianova, A.A., Sorokin, M.I., Bolosov, I.A., Buzdin, A.A., Kuzmin, D.V., and Ovchinnikova, T.V. (2018). Cytotoxic potential of the novel horseshoe crab peptide Polyphemusin III. Mar. Drugs, 16.","DOI":"10.3390\/md16120466"},{"key":"ref_27","first-page":"113","article-title":"Pharmaceuticals from cultured algae","volume":"5","author":"Schwartz","year":"1990","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"117017","DOI":"10.1016\/j.watres.2021.117017","article-title":"CyanoMetDB, a comprehensive public database of secondary metabolites from cyanobacteria","volume":"196","author":"Jones","year":"2021","journal-title":"Water Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1134\/S000368381707002X","article-title":"Isolation and characterization of toxic cyanobacteria from different natural sources","volume":"53","author":"Bolatkhan","year":"2017","journal-title":"Appl. Biochem. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"112364","DOI":"10.1016\/j.ejmech.2020.112364","article-title":"Cryptophycin-55\/52 based antibody-drug conjugates: Synthesis, efficacy, and mode of action studies","volume":"199","author":"Lai","year":"2020","journal-title":"Eur. J. Med. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1007\/s002800000210","article-title":"Cryptophycin-induced hyperphosphorylation of Bcl-2, cell cycle arrest and growth inhibition in human H460 NSCLC cells","volume":"47","author":"Lu","year":"2001","journal-title":"Cancer Chemother. Pharmacol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1377\/hlthaff.25.2.420","article-title":"Estimating the cost of new drug development: Is it really $802 million?","volume":"25","author":"Adams","year":"2006","journal-title":"Health Aff."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"197","DOI":"10.2741\/s509","article-title":"Microalgae as a source of high-value bioactive compounds","volume":"10","author":"Bule","year":"2018","journal-title":"Front. Biosci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.pbi.2009.09.010","article-title":"Actin and microtubule cytoskeleton interactions","volume":"12","year":"2009","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.ajpath.2019.11.016","article-title":"Endothelial glycocalyx impairment in disease: Focus on hyaluronan shedding","volume":"190","author":"Flamion","year":"2020","journal-title":"Am. J. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"101430","DOI":"10.1016\/j.gendis.2024.101430","article-title":"Oncogenic activation of PIK3CA in cancers: Emerging targeted therapies in precision oncology","volume":"12","author":"Wang","year":"2025","journal-title":"Genes Dis."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Santos, J.C., Profit\u00f3s-Pelej\u00e0, N., S\u00e1nchez-Vinces, S., and Rou\u00e9, G. (2023). RHOA Therapeutic Targeting in Hematological Cancers. Cells, 12.","DOI":"10.3390\/cells12030433"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4917","DOI":"10.1038\/s41388-022-02487-4","article-title":"Src: Coordinating metabolism in cancer","volume":"41","author":"Pelaz","year":"2022","journal-title":"Oncogene"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Liang, J., Oyang, L., Rao, S., Han, Y., Luo, X., Yi, P., Lin, J., Xia, L., Hu, J., and Tan, S. (2021). Rac1, a potential target for tumor therapy. Front. Oncol., 11.","DOI":"10.3389\/fonc.2021.674426"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Maldonado, M.D.M., Medina, J.I., Velazquez, L., and Dharmawardhane, S. (2020). Targeting Rac and Cdc42 GEFs in metastatic cancer. Front. Cell Dev. Biol., 8.","DOI":"10.3389\/fcell.2020.00201"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.algal.2018.06.008","article-title":"Description of a new species of soil algae, Parietochloris grandis sp. nov., and study of its fatty acid profiles under different culturing conditions","volume":"33","author":"Maltsev","year":"2018","journal-title":"Algal Res."},{"key":"ref_42","first-page":"10","article-title":"Yellow-green algae with chlorophyllide c","volume":"8","author":"Guillard","year":"1972","journal-title":"J. Phycol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Waterbury, J.B., and Stanier, R.Y. (1981). Isolation and growth of cyanobacteria from marine and hypersaline environments. The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria, Springer.","DOI":"10.1007\/978-3-662-13187-9_9"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1139\/m62-029","article-title":"Studies of marine planktonic diatoms: I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran","volume":"8","author":"Guillard","year":"1962","journal-title":"Can. J. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Guillard, R.R.L. (1975). Culture of phytoplankton for feeding marine invertebrates. Culture of Marine Invertebrate Animals, Springer.","DOI":"10.1007\/978-1-4615-8714-9_3"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.18632\/aging.101102","article-title":"Molecular pathway activation features of pediatric acute myeloid leukemia (AML) and acute lymphoblast leukemia (ALL) cells","volume":"8","author":"Petrov","year":"2016","journal-title":"Aging"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1038\/s41592-019-0686-2","article-title":"SciPy 1.0: Fundamental algorithms for scientific computing in Python","volume":"17","author":"Virtanen","year":"2020","journal-title":"Nat. Methods"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0022-1759(86)90368-6","article-title":"Rapid colorimetric assay for cell growth and survival: Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability","volume":"89","author":"Denizot","year":"1986","journal-title":"J. Immunol. Methods"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s10482-024-02008-1","article-title":"The genus Coelastrella (Chlorophyceae, Chlorophyta): Molecular species delimitation, biotechnological potential, and description of a new species Coelastrella affinis sp. nov., based on an integrative taxonomic approach","volume":"117","author":"Krivina","year":"2024","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1007\/s10811-018-1471-9","article-title":"Simultaneous increase in cellular content and volumetric concentration of lipids in Bracteacoccus bullatus cultivated at reduced nitrogen and phosphorus concentrations","volume":"30","author":"Mamaeva","year":"2018","journal-title":"J. Appl. Phycol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"109018","DOI":"10.1016\/j.bej.2023.109018","article-title":"Enhancement of carbon bio-fixation and lipid accumulation in Coccomyxa subellipsoidea with triethanolamine CO2 absorbent manipulation","volume":"198","author":"Li","year":"2023","journal-title":"Biochem. Eng. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.4014\/jmb.2209.09012","article-title":"Biotechnological approaches for biomass and lipid production using microalgae Chlorella and its future perspectives","volume":"32","author":"Je","year":"2022","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Liu, Y., Wei, D., and Chen, W. (2022). Oleaginous microalga Coccomyxa subellipsoidea as a highly effective cell factory for CO2 fixation and high-protein biomass production by optimal supply of inorganic carbon and nitrogen. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.921024"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1039\/D4NP00019F","article-title":"Progress in the discovery and development of anticancer agents from marine cyanobacteria","volume":"42","author":"Luesch","year":"2025","journal-title":"Nat. Prod. Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"805","DOI":"10.2174\/1389203724666230411091726","article-title":"Anticancer Compounds from Cyanobacteria and their Implications in Apoptosis","volume":"24","author":"Gupta","year":"2023","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Robles-Ba\u00f1uelos, B., Dur\u00e1n-Riveroll, L.M., Rangel-L\u00f3pez, E., P\u00e9rez-L\u00f3pez, H.I., and Gonz\u00e1lez-Maya, L. (2022). Marine Cyanobacteria as Sources of Lead Anticancer Compounds: A Review of Families of Metabolites with Cytotoxic, Antiproliferative, and Antineoplastic Effects. Molecules, 27.","DOI":"10.3390\/molecules27154814"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Ceg\u0142owska, M., Szubert, K., Grygier, B., Lenart, M., Plewka, J., Milewska, A., Lis, K., Szczepa\u0144ski, A., Chykunova, Y., and Barreto-Duran, E. (2022). Pseudanabaena galeata CCNP1313-Biological Activity and Peptides Production. Toxins, 14.","DOI":"10.3390\/toxins14050330"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.toxicon.2015.09.030","article-title":"Selective inhibition of cancer cells\u2019 proliferation by compounds included in extracts from Baltic Sea cyanobacteria","volume":"108","author":"Felczykowska","year":"2015","journal-title":"Toxicon"},{"key":"ref_60","first-page":"514","article-title":"Cryptophycins: Cytotoxic cyclodepsipeptides with potential for tumor targeting","volume":"23","author":"Weiss","year":"2017","journal-title":"J. Pept. Sci. Off. Publ. Eur. Pept. Soc."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e202402779","DOI":"10.1002\/cbdv.202402779","article-title":"Transcriptome Analysis Reveals Possible Antitumor Mechanism of Intracellular Polysaccharide from Phaeodactylum tricornutum on Cervical Cancer HeLa Cells","volume":"22","author":"Sun","year":"2025","journal-title":"Chem. Biodivers."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e6409","DOI":"10.7717\/peerj.6409","article-title":"Sulfated polysaccharides from Phaeodactylum tricornutum: Isolation, structural characteristics, and inhibiting HepG2 growth activity in vitro","volume":"7","author":"Yang","year":"2019","journal-title":"PeerJ"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.ejps.2015.12.001","article-title":"Screening of new antileukemic agents from essential oils of algae extracts and computational modeling of their interactions with intracellular signaling nodes","volume":"83","author":"Yilancioglu","year":"2016","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"M\u00e9resse, S., Fodil, M., Fleury, F., and Ch\u00e9nais, B. (2020). Fucoxanthin, a Marine-Derived Carotenoid from Brown Seaweeds and Microalgae: A Promising Bioactive Compound for Cancer Therapy. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21239273"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Du, H.F., Wu, J.W., Zhu, Y.S., Hua, Z.H., Jin, S.Z., Ji, J.C., Wang, C.S., Qian, G.Y., Jin, X.D., and Ding, H.M. (2024). Fucoxanthin Induces Ferroptosis in Cancer Cells via Downregulation of the Nrf2\/HO-1\/GPX4 Pathway. Molecules, 29.","DOI":"10.3390\/molecules29122832"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Lau, T.Y., and Kwan, H.Y. (2022). Fucoxanthin Is a Potential Therapeutic Agent for the Treatment of Breast Cancer. Mar. Drugs, 20.","DOI":"10.3390\/md20060370"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"3296","DOI":"10.1093\/jxb\/eraa091","article-title":"Zygnematophyceae: From living algae collections to the establishment of future models","volume":"71","author":"Zhou","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/nph.15334","article-title":"The genus Closterium, a new model organism to study sexual reproduction in streptophytes","volume":"221","author":"Tsuchikane","year":"2019","journal-title":"New Phytol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.1126\/science.1132939","article-title":"The Connectivity Map: Using gene-expression signatures to connect small molecules, genes, and disease","volume":"313","author":"Lamb","year":"2006","journal-title":"Science"}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/9\/1440\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T11:01:21Z","timestamp":1789124481000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/9\/1440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":69,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ph19091440"],"URL":"https:\/\/doi.org\/10.3390\/ph19091440","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}