{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:00:19Z","timestamp":1789984819976,"version":"4.0.1"},"reference-count":55,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Molius Sa\u011fl\u0131k Teknolojileri A.\u015e."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background: Sarcopenia is an aging-related syndrome characterized by loss of skeletal muscle mass, strength, and function, with no approved pharmacological treatment. This study aimed to design, synthesize, and evaluate novel ML-167 derivatives for sarcopenia-associated muscle wasting. Methods: ML-167 derivatives were synthesized and characterized by 1H NMR, 13C NMR, and mass spectrometry. Biological activity was evaluated in C2C12 myoblasts using MTT and Western blot assays. Selected compounds were evaluated in a dexamethasone-induced muscle wasting model. Muscle strength, mass, biochemical parameters, and histopathology were assessed. Structure\u2013activity relationships were analysed using cell viability, molecular docking, and ligand efficiency data, alongside physicochemical properties. Results: Compounds 6b (116%), 3a (115%), 8d (114%), 4c (112%), 3m (112%), and 3c (111%) showed the highest C2C12 viability at 1 \u03bcM. Compounds 3a, 3c, and 6b increased MyH3 expression by approximately 1.6\u20131.7-fold, while MyoG remained near basal levels. In vivo, these compounds improved muscle strength, mass, and biochemical parameters without overt hepatic or renal abnormalities based on assessed serum and histopathological findings. Among the evaluated derivatives, compound 6b emerged as the most promising overall lead candidate, showing the greatest improvement in skeletal muscle mass and metabolic parameters, whereas compound 3c demonstrated the strongest histopathological protection and favourable effects on muscle strength with favourable physicochemical properties. Molecular docking supported the SAR trends, although did not consistently correlate with cellular activity. Conclusions: The findings identify 3a, 3c, and 6b as promising lead compounds and support further investigation of ML-167 derivatives as potential pharmacological approaches for sarcopenia-associated muscle wasting.<\/jats:p>","DOI":"10.3390\/ph19091442","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:29:29Z","timestamp":1789133369000},"page":"1442","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design, Synthesis, and Biological Evaluation of Novel ML-167 Analogues as Therapeutic Candidates Against Sarcopenia-Induced Muscle Wasting"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3736-2545","authenticated-orcid":false,"given":"Issam Ameziane","family":"El Hassani","sequence":"first","affiliation":[{"name":"Trustlife Labs, Drug Research & Development Center, Istanbul 34774, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3601-807X","authenticated-orcid":false,"given":"Shabnam","family":"Alizadeh","sequence":"additional","affiliation":[{"name":"Trustlife Labs, Drug Research & Development Center, Istanbul 34774, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajda","family":"Ashraf","sequence":"additional","affiliation":[{"name":"Trustlife Labs, Drug Research & Development Center, Istanbul 34774, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6276-4124","authenticated-orcid":false,"given":"N. 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Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101319","DOI":"10.1016\/j.mam.2024.101319","article-title":"Muscle aging and sarcopenia: The pathology, etiology, and most promising therapeutic targets","volume":"100","author":"Campanario","year":"2024","journal-title":"Mol. Asp. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2636","DOI":"10.1016\/S0140-6736(19)31138-9","article-title":"Sarcopenia","volume":"393","author":"Sayer","year":"2019","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s11357-023-01016-9","article-title":"Sarcopenia: No consensus, no diagnostic criteria, and no approved indication\u2014How did we get here?","volume":"46","author":"Evans","year":"2023","journal-title":"GeroScience"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.3390\/ijms11041509","article-title":"Oxidative Stress, Molecular Inflammation and Sarcopenia","volume":"11","author":"Meng","year":"2010","journal-title":"Int. J. Mol. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"102267","DOI":"10.1016\/j.arr.2024.102267","article-title":"Restoration of epigenetic impairment in the skeletal muscle and chronic inflam-mation resolution as a therapeutic approach in sarcopenia","volume":"96","author":"Livshits","year":"2024","journal-title":"Ageing Res. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1042\/CS20230319","article-title":"Hallmarks of ageing in human skeletal muscle and implications for understanding the pathophysiology of sarcopenia in women and men","volume":"137","author":"Granic","year":"2023","journal-title":"Clin. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/s40520-016-0626-2","article-title":"Serum markers of inflammation and oxidative stress in sarcopenia","volume":"29","author":"Can","year":"2017","journal-title":"Aging Clin. Exp. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.taap.2010.10.006","article-title":"Sodium arsenite delays the differentiation of C2C12 mouse myoblast cells and alters methylation patterns on the transcription factor myogenin","volume":"250","author":"Steffens","year":"2011","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e649","DOI":"10.1002\/mco2.649","article-title":"Skeletal muscle: Molecular structure, myogenesis, biological functions, and diseases","volume":"5","author":"Feng","year":"2024","journal-title":"MedComm"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/s41526-023-00273-4","article-title":"Skeletal muscle gene expression dysregulation in long-term spaceflights and aging is clock-dependent","volume":"9","author":"Malhan","year":"2023","journal-title":"npj Microgravity"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kolonay, D.W., Sattler, K.M., Strawser, C., Rafael-Fortney, J., Mihaylova, M.M., Miller, K.E., Lepper, C., and Baskin, K.K. (2024). Temporal regulation of the Mediator complex during muscle proliferation, differentiation, regeneration, aging, and disease. Front. Cell Dev. Biol., 12.","DOI":"10.3389\/fcell.2024.1331563"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"116920","DOI":"10.1016\/j.biopha.2024.116920","article-title":"Novel drug targets and molecular mechanisms for sarcopenia based on systems biology","volume":"176","author":"Ceyhan","year":"2024","journal-title":"Biomed. Pharmacother."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Chen, K., Wang, S., Fu, S., Kim, J., Park, P., Liu, R., and Lei, K. (2025). 4(3H)-Quinazolinone: A Natural Scaffold for Drug and Agrochemical Discovery. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26062473"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1080\/00397911.2018.1431282","article-title":"Current perspectives on quinazolines with potent biological activities: A review","volume":"48","author":"Gupta","year":"2018","journal-title":"Synth. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2400057","DOI":"10.1002\/ardp.202400057","article-title":"A literature review on pharmacological aspects, docking studies, and synthetic approaches of quinazoline and quinazolinone derivatives","volume":"357","author":"Ghoneim","year":"2024","journal-title":"Arch. Pharm."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/jhet.4382","article-title":"Medicinal perspective of quinazolinone derivatives: Recent developments and structure\u2013activity relationship studies","volume":"59","author":"Wahan","year":"2022","journal-title":"J. Heterocycl. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1080\/17568919.2025.2539670","article-title":"The current landscape of quinazoline derivatives with in vivo anticancer therapeutic potential\u2013part II","volume":"17","author":"Huang","year":"2025","journal-title":"Future Med. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.2174\/1568026622666220307144015","article-title":"The Antibacterial Activity of Quinazoline and Quinazolinone Hybrids","volume":"22","author":"Li","year":"2022","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"41353","DOI":"10.1039\/D0RA06642G","article-title":"Recent advances in the pharmacological diversification of quinazoline\/quinazolinone hybrids","volume":"10","author":"Auti","year":"2020","journal-title":"RSC Adv."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1080\/17568919.2025.2507558","article-title":"Recent advances in the investigation of the quinazoline nucleus and derivatives with potential anticancer activities","volume":"17","author":"Antoniolli","year":"2025","journal-title":"Future Med. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Ashraf, S., Alizadeh, S., Hassani, I.A.E., Ozturk, A., Suer, N.C., Kim, W., Candan, G., Ayaz, G.B., Ozdemir, M., and Ye\u015filyurt, G. (2026). Synthesis, crystal structure, DFT, and molecular docking studies of quinazoline-based ML-167 derivatives. J. Mol. Struct., in press.","DOI":"10.1016\/j.molstruc.2026.147455"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.omtn.2023.02.003","article-title":"Identification of a novel small-molecule inhibitor of miR-29b attenuates muscle atrophy","volume":"31","author":"Liu","year":"2023","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"ref_24","first-page":"e23074","article-title":"Zeb1 and Tle3 are trans-factors that differentially regulate the expression of myosin heavy chain-embryonic and skeletal muscle differentiation","volume":"37","author":"Kumar","year":"2023","journal-title":"FASEB J. Off. Publ. Fed. Am. Soc. Exp. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"dev184507","DOI":"10.1242\/dev.184507","article-title":"Myosin heavy chain-embryonic regulates skeletal muscle differentiation during mammalian development","volume":"147","author":"Agarwal","year":"2020","journal-title":"Development"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/s00418-012-0972-x","article-title":"Myogenesis and muscle regeneration","volume":"138","author":"Yusuf","year":"2012","journal-title":"Histochem. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3714","DOI":"10.1021\/jm000942e","article-title":"Fast Calculation of Molecular Polar Surface Area as a Sum of Fragment-Based Contributions and Its Application to the Prediction of Drug Transport Properties","volume":"43","author":"Ertl","year":"2000","journal-title":"J. Med. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(96)00423-1","article-title":"Experimental and computational approaches to esti-mate solubility and permeability in drug discovery and development settings","volume":"23","author":"Lipinski","year":"1997","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_29","unstructured":"Di, L., and Kerns, E.H. (2015). Drug-Like Properties: Concepts, Structure Design and Methods from ADME to Toxicity Optimization, Academic press."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"101","DOI":"10.2174\/138620710790596763","article-title":"Solubility and Permeability Measurement and Applications in Drug Discovery","volume":"13","author":"Burton","year":"2010","journal-title":"CCHTS"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1007\/s00216-009-2634-y","article-title":"Analytical tools for the physicochemical profiling of drug candidates to predict absorption\/distribution","volume":"394","author":"Henchoz","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1186\/s40064-016-2427-7","article-title":"Dietary supplementation with shiikuwasha extract attenuates dexamethasone-induced skeletal muscle atrophy in aged rats","volume":"5","author":"Sakata","year":"2016","journal-title":"SpringerPlus"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Lim, S.-J., Kim, H.-J., Kim, H., Nam, H., Nam, K.-S., Kim, I., Kang, R., Hwang, I., and Kang, J.-S. (2025). Skeletal Muscle Atrophy Induced by Dexamethasone Is Attenuated by Amino Acid Complex Supplementation in Rats. Life, 15.","DOI":"10.3390\/life15040517"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kim, S., Kim, K., Park, J., and Jun, W. (2021). Curcuma longa L. Water extract improves dexamethasone-induced sarcopenia by modulating the muscle-related gene and oxidative stress in mice. Antioxidants, 10.","DOI":"10.3390\/antiox10071000"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"104985","DOI":"10.1016\/j.jff.2022.104985","article-title":"Yeast hydrolysate ameliorates dexamethasone-induced muscle atrophy by suppressing MuRF-1 expression in C2C12 cells and C57BL\/6 mice","volume":"90","author":"Chang","year":"2022","journal-title":"J. Funct. Foods"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Jia, H., Yamashita, T., Li, X., and Kato, H. (2022). Laurel attenuates dexamethasone-induced skeletal muscle atrophy in vitro and in a rat model. Nutrients, 14.","DOI":"10.3390\/nu14102029"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1002\/jcsm.12968","article-title":"Targeting MuRF1 by small molecules in a HFpEF rat model improves myocardial diastolic function and skeletal muscle contractility","volume":"13","author":"Adams","year":"2022","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"eabc8059","DOI":"10.1126\/science.abc8059","article-title":"Inhibition of prostaglandin-degrading enzyme 15-PGDH rejuvenates aged muscle mass and strength","volume":"371","author":"Palla","year":"2021","journal-title":"Science"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2300134","DOI":"10.1002\/bies.202300134","article-title":"Signaling roles of platelets in skeletal muscle regeneration","volume":"45","author":"Graca","year":"2023","journal-title":"BioEssays"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1056\/NEJMra035363","article-title":"Thrombocytosis","volume":"350","author":"Schafer","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3378","DOI":"10.1172\/JCI27196","article-title":"Platelets in inflammation and atherogenesis","volume":"115","author":"Gawaz","year":"2005","journal-title":"J. Clin. Investig."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Suzuki, A. (1998). Cross-Coupling Reactions of Organoboron Compounds with Organic Halides. Metal-Catalyzed Cross-Coupling Reactions, John Wiley & Sons, Ltd.","DOI":"10.1002\/9783527612222.ch2"},{"key":"ref_43","unstructured":"(Schr\u00f6dinger, 2023). Schr\u00f6dinger, Version 2023-3."},{"key":"ref_44","unstructured":"(Protein Preparation Wizard, Epik, Impact, and Prime, 2023). Protein Preparation Wizard, Epik, Impact, and Prime, Version 2."},{"key":"ref_45","unstructured":"(LigPrep, 2023). LigPrep, Version 2023-3."},{"key":"ref_46","unstructured":"Lesyk, D., and Kheilik, Y. (2026, July 27). Shake-Flask Aqueous Solubility Assay (Kinetic Solubility). Available online: https:\/\/www.protocols.io\/view\/shake-flask-aqueous-solubility-assay-kinetic-solub-djtz4np6.pdf."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4127","DOI":"10.1016\/j.bmc.2011.05.005","article-title":"Exploratory analysis of kinetic solubility measurements of a small molecule library","volume":"19","author":"Guha","year":"2011","journal-title":"Bioorg. Med. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.drudis.2011.11.007","article-title":"Bridging solubility between drug discovery and development","volume":"17","author":"Di","year":"2012","journal-title":"Drug Discov. Today"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.addr.2007.05.008","article-title":"Solubility of sparingly-soluble ionizable drugs","volume":"59","author":"Avdeef","year":"2007","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"98","DOI":"10.5599\/admet.760","article-title":"Estimation of the octanol-water distribution coefficient of basic compounds by a cationic microemulsion electrokinetic chromatography system","volume":"8","author":"Fuguet","year":"2020","journal-title":"ADMET DMPK"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jpba.2015.12.028","article-title":"A GC\u2013FID method for quantitative analysis of N, N-carbonyldiimidazole","volume":"121","author":"Lee","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1023\/A:1011954332221","article-title":"PH-Metric logP 10. Determination of Liposomal Membrane-Water Partition Coefficients of lonizable Drugs","volume":"15","author":"Avdeef","year":"1998","journal-title":"Pharm. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"42323","DOI":"10.1038\/srep42323","article-title":"Modified forelimb grip strength test detects aging-associated physiological decline in skeletal muscle function in male mice","volume":"7","author":"Takeshita","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"105521","DOI":"10.1016\/j.fbio.2024.105521","article-title":"Glycyrrhiza uralensis extract improves dexame-thasone-induced sarcopenia via regulating myostatin-and FoxO3a-mediated E3 ubiquitin ligase","volume":"62","author":"Zheng","year":"2024","journal-title":"Food Biosci."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Wu, W., Guo, X., Qu, T., Huang, Y., Tao, J., He, J., Wang, X., Luo, J., An, P., and Zhu, Y. (2024). The combination of lactoferrin and creatine ameliorates muscle decay in a sarcopenia murine model. Nutrients, 16.","DOI":"10.3390\/nu16121958"}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/9\/1442\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:03:52Z","timestamp":1789981432000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/9\/1442"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":55,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ph19091442"],"URL":"https:\/\/doi.org\/10.3390\/ph19091442","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}