{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:02:21Z","timestamp":1789707741006,"version":"4.0.1"},"reference-count":51,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Dasiphora parvifolia, a little-studied Central Asian shrub, lacks standardized pharmacognostic data. This study aimed to comprehensively characterize its above-ground organs, establish diagnostic criteria, comparatively evaluate extraction methods, profile phytochemicals, and evaluate antimicrobial activity. Methods: Morphological, morphometric, and anatomical analyses were performed using light microscopy (ANOVA, p \u2264 0.05). Physicochemical parameters were determined gravimetrically. Percolation and ultrasonic-assisted extraction (UAE) were compared using varying ethanol concentrations, ratios, and maceration times. Phenolics were identified by HPLC-UV and confirmed by ESI-MS. Results: Diagnostic features included leaflet size (6.91 \u00d7 1.14 mm), stem length (17.39 cm), and flower diameter (6.92 mm). Microscopy revealed anomocytic stomata and multicellular trichomes (120 \u00b5m). Quality parameters (moisture 2.61%, total ash 5.55%, extractives 42.36%). UAE with 70% ethanol (1:20, 24 h pre-maceration) gave 7.53% total phenolics in the dry extract and 7.68% for percolation, corresponding to 20.81 and 28.72 mg\/g of raw material, respectively. Among the analyzed extracts, the highest concentrations were detected for gallic acid (3.61%), chlorogenic acid (2.94%), cynaroside (2.28%), and hyperoside (1.82%). Total polyphenols, flavonoids, tannins, \u03b2-carotene, and \u03b1-tocopherol were 3.70%, 1.36%, 2.67%, 330.08 mg\/kg, and 138.0 mg%, respectively. DPPE70 showed the highest activity against P. aeruginosa (17.0 mm), whereas DPUE70-24 showed the highest activity against S. aureus (22.0 mm). Conclusions: D. parvifolia can be considered a relevant source of phenolic compounds. The high levels of gallic and chlorogenic acids, together with hyperoside and cynaroside, indicate a substantial phenolic profile and may contribute to the antioxidant and antimicrobial activities of the extracts.<\/jats:p>","DOI":"10.3390\/ph19091443","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:29:29Z","timestamp":1789133369000},"page":"1443","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dasiphora parvifolia: Pharmacognostic Standardization and Antimicrobial Potential"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1447-0850","authenticated-orcid":false,"given":"Aigul","family":"Medeshova","sequence":"first","affiliation":[{"name":"School of Pharmacy, Karaganda Medical University, Gogol 40, Karaganda 100000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1000-2719","authenticated-orcid":false,"given":"Aizhan","family":"Essedova","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Karaganda Medical University, Gogol 40, Karaganda 100000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1735-8290","authenticated-orcid":false,"given":"Margarita","family":"Ishmuratova","sequence":"additional","affiliation":[{"name":"Research Park of Biotechnology and Eco-Monitoring, Buketov Karaganda National Research University, Universitetskaya Street, 28, Karaganda 100028, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4314-6942","authenticated-orcid":false,"given":"Perizat","family":"Orazbayeva","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Karaganda Medical University, Gogol 40, Karaganda 100000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Belniak","sequence":"additional","affiliation":[{"name":"Department of Applied and Social Pharmacy, Faculty of Pharmacy, Medical University of Lublin, 1 Chod\u017aki Street, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6990-3664","authenticated-orcid":false,"given":"Sabira","family":"Sabyrova","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Karaganda Medical University, Gogol 40, Karaganda 100000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"ref_1","unstructured":"WHO (2003). 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