{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:04:04Z","timestamp":1789376644830,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Interuniversity Center for Healthy Aging","award":["RED211993"],"award-info":[{"award-number":["RED211993"]}]},{"name":"Interuniversity Center for Healthy Aging","award":["CIES007"],"award-info":[{"award-number":["CIES007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceuticals"],"abstract":"<jats:p>Background\/Objectives: Cellular senescence and oxidative stress contribute to macrophage dysfunction and chronic inflammation during aging. Buddleja globosa (matico) is traditionally consumed as an infusion and has reported antioxidant and anti-inflammatory properties. This study evaluated whether an aqueous extract of B. globosa (BgAE) modulates senescence-associated responses in THP-1-derived macrophages exposed to hydrogen peroxide. Methods: The phenolic profile of BgAE was analyzed by HPLC-DAD and its antioxidant capacity by the oxygen radical absorbance capacity assay. Cell viability was assessed after exposure to 0.01\u201350 \u00b5g\/mL BgAE. THP-1-derived macrophages were pretreated with BgAE for 48 h, exposed to 50 \u00b5M H2O2 for 1 h, and allowed to recover for 5 days. Senescence-associated \u03b2-galactosidase, inflammatory gene expression, and p16, p21, phosphorylated NF-\u03baB, and total NF-\u03baB protein levels were evaluated. Results: BgAE showed antioxidant capacity and did not significantly reduce cell viability under the conditions tested. H2O2 increased senescence-associated \u03b2-galactosidase positivity, TNFA expression, p16 and p21 abundance, and NF-\u03baB phosphorylation. BgAE reduced H2O2-induced \u03b2-galactosidase positivity at 1 and 50 \u00b5g\/mL and modulated TNFA, p16, p21, and NF-\u03baB responses, with effects varying across the concentrations evaluated. The 0.01 and 1 \u00b5g\/mL conditions generally showed greater attenuation of H2O2-induced changes, whereas 50 \u00b5g\/mL BgAE alone increased senescence-associated signaling. Conclusions: BgAE modulates selected senescence-associated and inflammatory responses in oxidatively stressed THP-1-derived macrophages. These findings provide initial in vitro evidence supporting further investigation and improved standardization of B. globosa aqueous preparations as modulators of oxidative stress-associated cellular responses.<\/jats:p>","DOI":"10.3390\/ph19091447","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:10:23Z","timestamp":1789373423000},"page":"1447","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Aqueous Extract of Buddleja globosa Modulates Oxidative Stress-Induced Senescence-Associated Responses in THP-1-Derived Macrophages"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8873-8699","authenticated-orcid":false,"given":"Roberto","family":"Bravo-Sagua","sequence":"first","affiliation":[{"name":"Laboratory of Obesity and Metabolism (OMEGA), Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos (INTA), Universidad de Chile, El L\u00edbano 5524, Macul, Santiago 7830490, Chile"},{"name":"Advanced Center for Chronic Diseases (ACCDiS), Universidad de Chile, Santiago 8380492, Chile"},{"name":"Interuniversity Center for Healthy Aging RED21993, Santiago 8320216, Chile"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Jakelin","family":"Gallardo","sequence":"additional","affiliation":[{"name":"Laboratory of Obesity and Metabolism (OMEGA), Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos (INTA), Universidad de Chile, El L\u00edbano 5524, Macul, Santiago 7830490, Chile"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"given":"Daniela","family":"Bergez","sequence":"additional","affiliation":[{"name":"Laboratory of Obesity and Metabolism (OMEGA), Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos (INTA), Universidad de Chile, El L\u00edbano 5524, Macul, Santiago 7830490, Chile"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6161-5998","authenticated-orcid":false,"given":"Alina","family":"Concepci\u00f3n-Alvarez","sequence":"additional","affiliation":[{"name":"Instituto de Nutrici\u00f3n y Tecnolog\u00eda de Los 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Chile"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9404-1193","authenticated-orcid":false,"given":"Adriano Costa","family":"de Camargo","sequence":"additional","affiliation":[{"name":"Instituto de Nutrici\u00f3n y Tecnolog\u00eda de Los Alimentos (INTA), Universidad de Chile, El L\u00edbano 5524, Macul, Santiago 7830490, Chile"},{"name":"Instituto de Ciencias Aplicadas, Universidad Aut\u00f3noma de Chile, Providencia, Santiago 7500910, 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Target. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1038\/s12276-025-01527-9","article-title":"Targeting Immunosenescence and Inflammaging: Advancing Longevity Research","volume":"57","author":"Nguyen","year":"2025","journal-title":"Exp. Mol. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1038\/s41574-018-0059-4","article-title":"Inflammaging: A New Immune\u2013Metabolic Viewpoint for Age-Related Diseases","volume":"14","author":"Franceschi","year":"2018","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1016\/j.apsb.2024.01.008","article-title":"Macrophage Senescence in Health and Diseases","volume":"14","author":"Wang","year":"2024","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1038\/s43587-024-00694-0","article-title":"Aged Bone Marrow Macrophages Drive Systemic Aging and Age-Related Dysfunction via Extracellular Vesicle-Mediated Induction of Paracrine Senescence","volume":"4","author":"Hou","year":"2024","journal-title":"Nat. Aging"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1038\/s43587-026-01101-6","article-title":"P21+TREM2+ Senescent Macrophages Fuel Inflammaging and Metabolic Dysfunction-Associated Steatotic Liver Disease","volume":"6","author":"Avila","year":"2026","journal-title":"Nat. Aging"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4150","DOI":"10.1016\/j.cell.2024.05.059","article-title":"Guidelines for Minimal Information on Cellular Senescence Experimentation in Vivo","volume":"187","author":"Ogrodnik","year":"2024","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2400079","DOI":"10.1002\/adbi.202400079","article-title":"Biomarkers of Cellular Senescence and Aging: Current State-of-the-Art, Challenges and Future Perspectives","volume":"8","author":"Muthamil","year":"2024","journal-title":"Adv. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100102","DOI":"10.1016\/j.mocell.2024.100102","article-title":"Brief Guide to Senescence Assays Using Cultured Mammalian Cells","volume":"47","author":"Kang","year":"2024","journal-title":"Mol. Cells"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102832","DOI":"10.1016\/j.arr.2025.102832","article-title":"Apheresis for Senescence: Targeting the Senescence-Associated Secretory Phenotype to Delay Aging and Age-Related Diseases","volume":"111","author":"Akgun","year":"2025","journal-title":"Ageing Res. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1038\/s41580-024-00738-8","article-title":"SenNet Recommendations for Detecting Senescent Cells in Different Tissues","volume":"25","author":"Suryadevara","year":"2024","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"102558","DOI":"10.1016\/j.arr.2024.102558","article-title":"Oxidative Stress and Cell Senescence as Drivers of Ageing: Chicken and Egg","volume":"102","year":"2024","journal-title":"Ageing Res. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Stojanovic, B., Jovanovic, I., Dimitrijevic Stojanovic, M., Stojanovic, B.S., Kovacevic, V., Radosavljevic, I., Jovanovic, D., Miletic Kovacevic, M., Zornic, N., and Arsic, A.A. (2025). Oxidative Stress-Driven Cellular Senescence: Mechanistic Crosstalk and Therapeutic Horizons. Antioxidants, 14.","DOI":"10.3390\/antiox14080987"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kumari, R., and Jat, P. (2021). Mechanisms of Cellular Senescence: Cell Cycle Arrest and Senescence Associated Secretory Phenotype. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.645593"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"102619","DOI":"10.1016\/j.arr.2024.102619","article-title":"The Senotherapeutic Potential of Phytochemicals for Age-Related Intestinal Disease","volume":"104","author":"Costa","year":"2025","journal-title":"Ageing Res. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1172\/JCI95148","article-title":"Mechanisms and Functions of Cellular Senescence","volume":"128","author":"Herranz","year":"2018","journal-title":"J. Clin. Investig."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Becerra, \u00c1., Soto, F., Mill\u00e1n, D., Valenzuela-Fuenzalida, J.J., Moya, M.P., Le\u00f3n-Rojas, J.E., and Cort\u00e9s, M.E. (2026). Antioxidant and Anti-Inflammatory Properties of Buddleja Globosa Hope (Matico): A Systematic Review of Phytochemical Composition, Molecular Mechanisms, and Translational Evidence. Antioxidants, 15.","DOI":"10.3390\/antiox15070790"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Otero, C., Klagges, C., Morales, B., Sotomayor, P., Escobar, J., Fuentes, J.A., Moreno, A.A., Llancalahuen, F.M., Arratia-Perez, R., and Gordillo-Fuenzalida, F. (2023). Anti-Inflammatory Chilean Endemic Plants. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15030897"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Torres-Vega, J., G\u00f3mez-Alonso, S., P\u00e9rez-Navarro, J., Alarc\u00f3n-Enos, J., and Pastene-Navarrete, E. (2021). Polyphenolic Compounds Extracted and Purified from Buddleja Globosa Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography. Molecules, 26.","DOI":"10.3390\/molecules26082192"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Varesi, A., Chirumbolo, S., Campagnoli, L.I.M., Pierella, E., Piccini, G.B., Carrara, A., Ricevuti, G., Scassellati, C., Bonvicini, C., and Pascale, A. (2022). The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence. Antioxidants, 11.","DOI":"10.3390\/antiox11071224"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Sun, S., Yu, Y., Jo, Y., Han, J.H., Xue, Y., Cho, M., Bae, S.-J., Ryu, D., Park, W., and Ha, K.-T. (2025). Impact of Extraction Techniques on Phytochemical Composition and Bioactivity of Natural Product Mixtures. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1615338"},{"key":"ref_22","first-page":"2201","article-title":"Advancements in Targeting Macrophage Senescence for Age-Associated Conditions","volume":"16","author":"Xiao","year":"2024","journal-title":"Aging Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"12697","DOI":"10.18632\/aging.206124","article-title":"A New Model and Precious Tool to Study Molecular Mechanisms of Macrophage Aging","volume":"16","author":"Smith","year":"2024","journal-title":"Aging"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s10522-026-10419-y","article-title":"Epigallocatechin Gallate Mitigates Oxidative Stress-Induced Transient Senescence and Injury by Preserving Mitochondrial Integrity and Restoring Redox-Inflammatory Homeostasis in Murine Macrophages","volume":"27","author":"Kumar","year":"2026","journal-title":"Biogerontology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1002\/tox.23711","article-title":"Rutin Alleviates Bisphenol A-glycidyl Methacrylate-induced Generation of Proinflammatory Mediators through the MAPK and NF-\u03baB Pathways in Macrophages","volume":"38","author":"Huang","year":"2023","journal-title":"Environ. Toxicol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s11033-025-10232-9","article-title":"Comparative Evaluation of Cellular Senescence in Naturally Aged and Stress-Induced Murine Macrophages for Identifying Optimum Senescent Macrophage Study Systems","volume":"52","author":"Danish","year":"2025","journal-title":"Mol. Biol. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.bbamem.2012.08.013","article-title":"Effect of Oxidative Stress on Plasma Membrane Fluidity of THP-1 Induced Macrophages","volume":"1828","author":"Palacio","year":"2013","journal-title":"Biochim. Et Biophys. Acta (BBA)\u2014Biomembr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1038\/s41580-024-00727-x","article-title":"The Senescence-Associated Secretory Phenotype and Its Physiological and Pathological Implications","volume":"25","author":"Wang","year":"2024","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Beltran-Velasco, A.I., and Clemente-Su\u00e1rez, V.J. (2025). Impact of Peripheral Inflammation on Blood\u2013Brain Barrier Dysfunction and Its Role in Neurodegenerative Diseases. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26062440"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kwiatkowska, K.M., Mavrogonatou, E., Papadopoulou, A., Sala, C., Calzari, L., Gentilini, D., Bacalini, M.G., Dall\u2019Olio, D., Castellani, G., and Ravaioli, F. (2023). Heterogeneity of Cellular Senescence: Cell Type-Specific and Senescence Stimulus-Dependent Epigenetic Alterations. Cells, 12.","DOI":"10.3390\/cells12060927"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"157425","DOI":"10.1016\/j.phymed.2025.157425","article-title":"Reversal of BCAA-Driven Inflammatory Senescence by Traditional Herbal Oil Prevents Atopic Dermatitis Relapse","volume":"148","author":"Wang","year":"2025","journal-title":"Phytomedicine"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S0378-8741(01)00297-5","article-title":"Effects of Buddleja Globosa Leaf and Its Constituents Relevant to Wound Healing","volume":"77","author":"Mensah","year":"2001","journal-title":"J. Ethnopharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"112828","DOI":"10.1016\/j.biopha.2022.112828","article-title":"Chemical Characterization of Extracts of Leaves of Kadsua Coccinea (Lem.) A.C. Sm. by UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Assessment of Their Antioxidant and Anti-Inflammatory Activities","volume":"149","author":"Shi","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Tedesco, S., De Majo, F., Kim, J., Trenti, A., Trevisi, L., Fadini, G.P., Bolego, C., Zandstra, P.W., Cignarella, A., and Vitiello, L. (2018). Convenience versus Biological Significance: Are PMA-Differentiated THP-1 Cells a Reliable Substitute for Blood-Derived Macrophages When Studying in Vitro Polarization?. Front. Pharmacol., 9.","DOI":"10.3389\/fphar.2018.00071"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1039\/C9NP00011A","article-title":"Synergy and Antagonism in Natural Product Extracts: When 1 + 1 Does Not Equal 2","volume":"36","author":"Caesar","year":"2019","journal-title":"Nat. Prod. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4067\/S0717-66432018000100001","article-title":"Cat\u00e1logo de Las Plantas Vasculares de Chile","volume":"75","author":"Rodriguez","year":"2018","journal-title":"Gayana Bot."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bridi, R., Echeverr\u00eda, J., Larena, A., Nu\u00f1ez Pizarro, P., Atala, E., De Camargo, A.C., Oh, W.Y., Shahidi, F., Garcia, O., and Ah-Hen, K.S. (2022). Honeybee Pollen From Southern Chile: Phenolic Profile, Antioxidant Capacity, Bioaccessibility, and Inhibition of DNA Damage. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.775219"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4026","DOI":"10.1021\/jf049696w","article-title":"Lipophilic and Hydrophilic Antioxidant Capacities of Common Foods in the United States","volume":"52","author":"Wu","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.2174\/1871530320666200513082300","article-title":"Hydroethanolic Extract of Lampaya Medicinalis Phil. (Verbenaceae) Decreases Proinflammatory Marker Expression in Palmitic Acid-Exposed Macrophages","volume":"20","author":"Ormazabal","year":"2020","journal-title":"Endocr. Metab. Immune Disord. Drug Targets"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A New Mathematical Model for Relative Quantification in Real-Time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."}],"container-title":["Pharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/9\/1447\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:13:40Z","timestamp":1789373620000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8247\/19\/9\/1447"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,12]]},"references-count":40,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ph19091447"],"URL":"https:\/\/doi.org\/10.3390\/ph19091447","relation":{},"ISSN":["1424-8247"],"issn-type":[{"value":"1424-8247","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,12]]}}}