{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:00:15Z","timestamp":1777510815745,"version":"3.51.4"},"reference-count":34,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,8,19]],"date-time":"2019-08-19T00:00:00Z","timestamp":1566172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016H1D3A1938159"],"award-info":[{"award-number":["2016H1D3A1938159"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1A6A1A03025523"],"award-info":[{"award-number":["2018R1A6A1A03025523"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017R1A2A2A07001272"],"award-info":[{"award-number":["2017R1A2A2A07001272"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2019M3E5D1A02069623"],"award-info":[{"award-number":["2019M3E5D1A02069623"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>In this study, we fabricated a doxycycline (doxy)-eluting nanofiber-covered endotracheal stent for the prevention of stent intubation-related tissue fibrosis and re-stenosis. The nanofiber was deposited directly on the outer surface of the stent using a coaxial electrospinning method to form a doxy-eluting cover sleeve. Poly(d,l-lactide) was used as the shell-forming polymer and dedicated drug release-control membrane. Polyurethane was selected as the drug-loading core polymer. The compositional ratio of the core to shell was adjusted to 1:0, 1:2, and 1:4 by changing the electro-spray rate of each polymeric solution and microscopic observation of nanofibers using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the fluorescence microscopy proved core-shell structure of nanofibers. The in vitro release study suggested that the release of doxy could be controlled by increasing the compositional ratio of the shell. The growth of HT1080 fibrosarcoma cells was inhibited by the 10% doxy-containing nanofiber. The real-time polymerase chain reaction (PCR) in HT1080 cells and xenografted tissue models indicated that the doxy-releasing nanofiber inhibited mRNA expression of metalloproteinases (MT1-MMP, MMP-2, and MMP-9). Overall, our study demonstrates that a doxy-eluting core-shell nanofiber stent can be successfully fabricated using coaxial electrospinning and displays the potential to prevent fibrotic re-stenosis, which is the most problematic clinical complication of tracheal stent intubation.<\/jats:p>","DOI":"10.3390\/pharmaceutics11080421","type":"journal-article","created":{"date-parts":[[2019,8,19]],"date-time":"2019-08-19T11:22:38Z","timestamp":1566213758000},"page":"421","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Doxycycline-Eluting Core-Shell Type Nanofiber-Covered Trachea Stent for Inhibition of Cellular Metalloproteinase and Its Related Fibrotic Stenosis"],"prefix":"10.3390","volume":"11","author":[{"given":"Rengarajan","family":"Baskaran","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, Inha University College of Medicine, Incheon 22332, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Un-Jeong","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Inha University College of Medicine, Incheon 22332, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enkhzaya","family":"Davaa","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Inha University College of Medicine, Incheon 22332, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Eun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Inha University College of Medicine, Incheon 22332, Korea"},{"name":"Inha Institute of Aerospace Medicine, Inha University College of Medicine, Incheon 22332, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Inha University College of Medicine, Incheon 22332, Korea"},{"name":"Inha Institute of Aerospace Medicine, Inha University College of Medicine, Incheon 22332, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junghan","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Inha University College of Medicine, Incheon 22332, Korea"},{"name":"Inha Institute of Aerospace Medicine, Inha University College of Medicine, Incheon 22332, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su-Geun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Inha University College of Medicine, Incheon 22332, Korea"},{"name":"Inha Institute of Aerospace Medicine, Inha University College of Medicine, Incheon 22332, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1136\/bmj.321.7267.1000","article-title":"Tracheal stenosis after intubation","volume":"321","author":"Spittle","year":"2000","journal-title":"BMJ"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/S0003-4975(00)01614-3","article-title":"Malignant laryngotracheal obstruction: A way to treat serial stenoses of the upper airways","volume":"70","author":"Wassermann","year":"2000","journal-title":"Ann. 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