{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:37:37Z","timestamp":1789159057659,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T00:00:00Z","timestamp":1580688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bando Cluster Tecnologici Nazionali-Progetto IRMI","award":["CTN01_00177_88744"],"award-info":[{"award-number":["CTN01_00177_88744"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Standard treatments of chronic skin ulcers based on the direct application of dressings still present several limits with regard to a complete tissue regeneration. Innovative strategies in tissue engineering offer materials that can tune cell behavior and promote growth tissue favoring cell recruitment in the early stages of wound healing. A combination of Alginate (Alg), Sericin (SS) with Platelet Lysate (PL), as a freeze-dried sponge, is proposed to generate a bioactive wound dressing to care skin lesions. Biomembranes at different composition were tested for the release of platelet growth factors, cytotoxicity, protective effects against oxidative stress and cell proliferation induction. The highest level of the growth factors release occurred within 48 h, an optimized time to burst a healing process in vivo; the presence of SS differently modulated the release of the factors by interaction with the proteins composing the biomembranes. Any cytotoxicity was registered, whereas a capability to protect cells against oxidative stress and induce proliferation was observed when PL was included in the biomembrane. In a mouse skin lesion model, the biomembranes with PL promoted the healing process, inducing an accelerated and more pronounced burst of inflammation, formation of granulation tissue and new collagen deposition, leading to a more rapid skin regeneration.<\/jats:p>","DOI":"10.3390\/pharmaceutics12020120","type":"journal-article","created":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T02:59:18Z","timestamp":1580957958000},"page":"120","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Growth Factors Delivery System for Skin Regeneration: An Advanced Wound Dressing"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4858-6480","authenticated-orcid":false,"given":"Marta","family":"Nardini","sequence":"first","affiliation":[{"name":"Department of Internal Medicine (DIMI), University of Genova, viale Benedetto XV 10, 16132 Genova, Italy"},{"name":"Biotherapy Unit, Ospedale Policlinico San Martino IRCCS, largo Rosanna Benzi 10, 16132 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1128-7636","authenticated-orcid":false,"given":"Sara","family":"Perteghella","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, University of Pavia, viale Taramelli 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Mastracci","sequence":"additional","affiliation":[{"name":"Ospedale Policlinico San Martino IRCCS, largo Rosanna Benzi 10, 16132 Genova, Italy"},{"name":"Anatomic Pathology, Department of Integrated Surgical and Diagnostic Sciences (DISC), University of Genova, viale Benedetto XV 6, 16132 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Grillo","sequence":"additional","affiliation":[{"name":"Ospedale Policlinico San Martino IRCCS, largo Rosanna Benzi 10, 16132 Genova, Italy"},{"name":"Anatomic Pathology, Department of Integrated Surgical and Diagnostic Sciences (DISC), University of Genova, viale Benedetto XV 6, 16132 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1399-5783","authenticated-orcid":false,"given":"Giorgio","family":"Marrubini","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, University of Pavia, viale Taramelli 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0241-0620","authenticated-orcid":false,"given":"Elia","family":"Bari","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, University of Pavia, viale Taramelli 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2388-9693","authenticated-orcid":false,"given":"Matteo","family":"Formica","sequence":"additional","affiliation":[{"name":"Ospedale Policlinico San Martino IRCCS, largo Rosanna Benzi 10, 16132 Genova, Italy"},{"name":"Orthopedic Clinic, Department of Integrated Surgical and Diagnostic Sciences (DISC), University of Genova viale Benedetto XV 6, 16132 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Gentili","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory, Department of Experimental Medicine (DIMES), University of Genova, via Leon Battista Alberti 2, 16132 Genova, Italy"},{"name":"Center for Biomedical Research (CEBR), University of Genova, viale Benedetto XV 9, 16132 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranieri","family":"Cancedda","sequence":"additional","affiliation":[{"name":"Endolife S.r.l., Piazza della Vittoria 15\/23, 16121 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8062-6836","authenticated-orcid":false,"given":"Maria Luisa","family":"Torre","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, University of Pavia, viale Taramelli 12, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2647-4090","authenticated-orcid":false,"given":"Maddalena","family":"Mastrogiacomo","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine (DIMI), University of Genova, viale Benedetto XV 10, 16132 Genova, Italy"},{"name":"Biotherapy Unit, Ospedale Policlinico San Martino IRCCS, largo Rosanna Benzi 10, 16132 Genova, Italy"},{"name":"Center for 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