{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:49:42Z","timestamp":1791967782268,"version":"4.3.4"},"reference-count":79,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T00:00:00Z","timestamp":1610755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004343","name":"Stavros Niarchos Foundation","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100004343","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005411","name":"Foundation for Education and European Culture","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100005411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>In this work, novel chrysin-loaded poly(\u03b5-caprolactone) and poly(3-hydroxybutyrate) microcarriers were synthesized according to a modified oil-in-water single emulsion\/solvent evaporation method, utilizing poly(vinyl alcohol) surfactant as stabilizer and dispersing agent for the emulsification, and were evaluated for their physico-chemical and morphological properties, loading capacity and entrapment efficiency and in vitro release of their load. The findings suggest that the novel micro-formulations possess a spherical and relatively wrinkled structure with sizes ranging between 2.4 and 24.7 \u00b5m and a highly negative surface charge with z-potential values between (\u221218.1)\u2013(\u221214.1) mV. The entrapment efficiency of chrysin in the poly(\u03b5-caprolactone) and poly(3-hydroxybutyrate) microcarriers was estimated to be 58.10% and 43.63%, whereas the loading capacity was found to be 3.79% and 15.85%, respectively. The average release percentage of chrysin was estimated to be 23.10% and 18.01%, respectively. The novel micromaterials were further biologically evaluated for their hemolytic activity through hemocompatibility studies over a range of hematological parameters and cytoxicity against the epithelial human breast cancer cell line MDA-MB 231. The poly(\u03b5-caprolactone) and poly(3-hydroxybutyrate) microcarriers reached an IC50 value with an encapsulated chrysin content of 149.19 \u00b5M and 312.18 \u00b5M, respectively, and showed sufficient blood compatibility displaying significantly low (up to 2%) hemolytic percentages at concentrations between 5 and 500 \u00b5g\u00b7mL\u22121.<\/jats:p>","DOI":"10.3390\/pharmaceutics13010109","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T03:34:25Z","timestamp":1611113665000},"page":"109","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Evaluation of the Hemocompatibility and Anticancer Potential of Poly(\u03b5-Caprolactone) and Poly(3-Hydroxybutyrate) Microcarriers with Encapsulated Chrysin"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6803-5395","authenticated-orcid":false,"given":"Eleftherios","family":"Halevas","sequence":"first","affiliation":[{"name":"Institute of Biosciences &amp; Applications, National Centre for Scientific Research \u201cDemokritos\u201d, 15310 Athens, Greece"},{"name":"Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4825-9022","authenticated-orcid":false,"given":"Chrysoula","family":"Kokotidou","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Technology, University of Crete, Voutes Campus, 70013 Heraklion, Greece"},{"name":"Institute for Electronic Structure and Laser FORTH, N. Plastira 100, 70013 Heraklion, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elda","family":"Zaimai","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Moschona","sequence":"additional","affiliation":[{"name":"Laboratory of Organic Chemistry, Department of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"},{"name":"Laboratory of Natural Resources and Renewable Energies, Chemical Process and Energy Resources Institute, Centre for Research and Technology-Hellas (CERTH), 6th km Harilaou-Thermis, 57001 Thermi, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efstratios","family":"Lialiaris","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Medical School, Democritus University of Thrace, 68100 Alexandroupolis, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Mitraki","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Technology, University of Crete, Voutes Campus, 70013 Heraklion, Greece"},{"name":"Institute for Electronic Structure and Laser FORTH, N. 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