{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T12:43:02Z","timestamp":1775306582716,"version":"3.50.1"},"reference-count":75,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-15-00108"],"award-info":[{"award-number":["19-15-00108"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["\u0410\u0410\u0410\u0410-\u041019-119021290033-1"],"award-info":[{"award-number":["\u0410\u0410\u0410\u0410-\u041019-119021290033-1"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011051","name":"Council on grants of the President of the Russian Federation","doi-asserted-by":"publisher","award":["SP-822.2018.4"],"award-info":[{"award-number":["SP-822.2018.4"]}],"id":[{"id":"10.13039\/501100011051","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Uterine leiomyoma (UL) is one of the most common benign tumors in women that often leads to many reproductive complications. Suicide genetherapy was suggested as a promising approach for UL treatment. In the present study, we describe iRGD ligand-conjugated cysteine-rich peptide carrier RGD1-R6 for targeted DNA delivery to \u03b1v\u03b23 integrin-expressing primary UL cells. The physico-chemical properties, cytotoxicity, transfection efficiency and specificity of DNA\/RGD1-R6 polyplexes were investigated. TheHSV-1thymidine kinase encoding plasmid delivery to PANC-1pancreatic carcinoma cells and primary UL cells resulted in significant suicide gene therapy effects. Subsequent ganciclovir treatment decreased cells proliferative activity, induced of apoptosis and promoted cells death.The obtained results allow us to concludethatthe developed RGD1-R6 carrier can be considered a promising candidate for suicide gene therapy of uterine leiomyoma.<\/jats:p>","DOI":"10.3390\/pharmaceutics13020202","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T13:01:12Z","timestamp":1612270872000},"page":"202","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development of iRGD-Modified Peptide Carriers for Suicide Gene Therapy of Uterine Leiomyoma"],"prefix":"10.3390","volume":"13","author":[{"given":"Anna","family":"Egorova","sequence":"first","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Shtykalova","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Selutin","sequence":"additional","affiliation":[{"name":"Department of Immunology and Intercellular Interactions, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Shved","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianna","family":"Maretina","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergei","family":"Selkov","sequence":"additional","affiliation":[{"name":"Department of Immunology and Intercellular Interactions, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladislav","family":"Baranov","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2487-2423","authenticated-orcid":false,"given":"Anton","family":"Kiselev","sequence":"additional","affiliation":[{"name":"Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya Line 3, 199034 Saint-Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3109\/19396368.2015.1123325","article-title":"Comparative systems genetics view of endometriosis and uterine leiomyoma: Two sides of the same coin?","volume":"62","author":"Baranov","year":"2016","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2398","DOI":"10.1093\/humrep\/det275","article-title":"Targeting adenoviral vectors for enhanced gene therapy of uterine leiomyomas","volume":"28","author":"Nair","year":"2013","journal-title":"Hum. Reprod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S0015-0282(00)01714-3","article-title":"Impact of intramural leiomyomata in patients with a normal endometrial cavity on in vitro fertilization\u2013embryo transfer cycle outcome","volume":"75","author":"Surrey","year":"2001","journal-title":"Fertil. Steril."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1093\/annonc\/mdn164","article-title":"Practical guidance for the management of aromatase inhibitor-associated bone loss","volume":"19","author":"Hadji","year":"2008","journal-title":"Ann. Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1093\/biolre\/iox094","article-title":"Selective progesterone receptor modulators for fertility preservation in women with symptomatic uterine fibroids","volume":"97","author":"Ali","year":"2017","journal-title":"Biol. Reprod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1093\/humupd\/dml015","article-title":"Gene therapy and uterine leiomyoma: A review","volume":"12","author":"Salama","year":"2006","journal-title":"Hum. Reprod. Update"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/S0889-8545(21)00664-1","article-title":"Abdominal myomectomy as a treatment for symptomatic uterine fibroids","volume":"22","author":"Hutchins","year":"1995","journal-title":"Obstet. Gynecol. Clin. N. Am."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Gwak, S.J., and Lee, J.S. (2019). Suicide gene therapy by amphiphilic copolymer nanocarrier for spinal cord tumor. Nanomaterials, 9.","DOI":"10.3390\/nano9040573"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/sj.cgt.7700844","article-title":"Folate-linked nanoparticle-mediated suicide gene therapy in human prostate cancer and nasopharyngeal cancer with herpes simplex virus thymidine kinase","volume":"12","author":"Hattori","year":"2005","journal-title":"Cancer Gene Ther."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9766","DOI":"10.1016\/j.biomaterials.2011.08.089","article-title":"Biomaterials Suicide gene therapy using reducible poly (oligo-d-arginine) for the treatment of spinal cord tumors","volume":"32","author":"Won","year":"2011","journal-title":"Biomaterials"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"13","DOI":"10.2174\/1566523033347426","article-title":"Suicide gene therapy mediated by the herpes simplex virus thymidine kinase Gene\/ganciclovir system: Fifteen years of application","volume":"3","author":"Fillat","year":"2003","journal-title":"Curr. Gene Ther."},{"key":"ref_12","first-page":"735","article-title":"Nonviral vector-mediated thymidine kinase gene transfer and ganciclovir treatment in leiomyoma cells","volume":"91","author":"Niu","year":"1998","journal-title":"Obstet. Gynecol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.1016\/j.fertnstert.2016.03.001","article-title":"Magnetic nanoparticles as a new approach to improve the efficacy of gene therapy against differentiated human uterine fibroid cells and tumor-initiating stem cells","volume":"105","author":"Shalaby","year":"2016","journal-title":"Fertil. Steril."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/s41417-019-0101-2","article-title":"Targeted AAVP-based therapy in a mouse model of human glioblastoma: A comparison of cytotoxic versus suicide gene delivery strategies","volume":"27","author":"Staquicini","year":"2020","journal-title":"Cancer Gene Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/s41573-019-0012-9","article-title":"Adeno-associated virus vector as a platform for gene therapy delivery","volume":"18","author":"Wang","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.2217\/nnm.14.90","article-title":"Peptide vectors for gene delivery: From single peptides to multifunctional peptide nanocarriers","volume":"9","author":"Teunissen","year":"2014","journal-title":"Nanomedicine"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.2217\/nnm.15.115","article-title":"RALA-mediated delivery of FKBPL nucleic acid therapeutics","volume":"10","author":"Bennett","year":"2015","journal-title":"Nanomedicine"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.addr.2017.07.021","article-title":"Nonviral cancer gene therapy: Delivery cascade and vector nanoproperty integration","volume":"115","author":"Zhou","year":"2017","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"9","DOI":"10.2217\/nnm-2017-0206","article-title":"Modification of degradable nonviral delivery vehicle with a novel bifunctional peptide to enhance transfection in vivo","volume":"13","author":"Zhang","year":"2018","journal-title":"Nanomedicine"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110173","DOI":"10.1016\/j.micromeso.2020.110173","article-title":"Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks","volume":"300","author":"Abdelhamid","year":"2020","journal-title":"Microporous Mesoporous Mater."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Langlet-bertin, B., Leborgne, C., Scherman, D., Bechinger, B., Mason, A.J., and Kichler, A. (2010). Design and evaluation of histidine-rich amphipathic peptides for siRNA delivery. Pharm. Res., 1426\u20131436.","DOI":"10.1007\/s11095-010-0138-2"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"330","DOI":"10.2174\/1568026615666150812120755","article-title":"Peptide modules for overcoming barriers of nucleic acids transport to cells","volume":"16","author":"Egorova","year":"2016","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.tips.2017.01.003","article-title":"Cell-penetrating peptides: From basic research to clinics","volume":"38","author":"Guidotti","year":"2017","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.bmc.2017.12.013","article-title":"Bombesin\/oligoarginine fusion peptides for gastrin releasing peptide receptor (GRPR) targeted gene delivery","volume":"26","author":"Begum","year":"2018","journal-title":"Bioorgan. Med. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"19926","DOI":"10.1038\/s41598-019-56455-2","article-title":"Effective lung-targeted RNAi in mice with peptide-based delivery of nucleic acid","volume":"9","author":"Kurrikoff","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.bmcl.2015.11.052","article-title":"Cysteine and arginine-rich peptides as molecular carriers","volume":"26","author":"Shirazi","year":"2016","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1039\/C7BM00584A","article-title":"Peptides, polypeptides and peptide-polymer hybrids as nucleic acid carriers","volume":"5","author":"Ahmed","year":"2017","journal-title":"Biomater. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6913","DOI":"10.2147\/IJN.S180957","article-title":"Peptide T7-modified polypeptide with disulfide bonds for targeted delivery of plasmid DNA for gene therapy of prostate cancer","volume":"13","author":"Lu","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1016\/j.ijpharm.2012.10.020","article-title":"Characterization of reducible peptide oligomers as carriers for gene delivery","volume":"441","author":"Kiselev","year":"2013","journal-title":"Int. J. Pharm."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jconrel.2011.01.030","article-title":"Glutathione-responsive nano-vehicles as a promising platform for targeted intracellular drug and gene delivery","volume":"152","author":"Cheng","year":"2011","journal-title":"J. Control. Release"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.bbamem.2006.02.005","article-title":"Cationic amphipathic histidine-rich peptides for gene delivery","volume":"1758","author":"Kichler","year":"2006","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ijpharm.2019.05.052","article-title":"Design and evaluation of ionizable peptide amphiphiles for siRNA delivery","volume":"566","author":"Neuberg","year":"2019","journal-title":"Int. J. Pharm."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4476","DOI":"10.1021\/acs.jpcb.0c01853","article-title":"Characterization of the DNA and Membrane Interactions of a Bioreducible Cell-Penetrating Foldamer in its Monomeric and Dimeric Form","volume":"124","author":"Aisenbrey","year":"2020","journal-title":"J. Phys. Chem. B"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/j.bmcl.2019.01.043","article-title":"Design and synthesis of novel dual-cyclic RGD peptides for \u03b1v\u03b23 integrin targeting","volume":"29","author":"Liu","year":"2019","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1055\/s-0030-1251475","article-title":"Why leiomyomas are called fibroids: The central role of extracellular matrix in symptomatic women","volume":"28","author":"Malik","year":"2010","journal-title":"Semin. Reprod. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/0076-6879(87)44196-7","article-title":"[27] Arginine-glycine-aspartic acid adhesion receptors","volume":"144","author":"Pytela","year":"1987","journal-title":"Methods Enzymol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7543","DOI":"10.1016\/j.biomaterials.2014.05.021","article-title":"Tumor targeting RGD conjugated bio-reducible polymer for VEGF siRNA expressing plasmid delivery","volume":"35","author":"Kim","year":"2014","journal-title":"Biomaterials"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.jddst.2016.12.005","article-title":"RGD-derivatized PEI-PEG copolymers: Influence of the degree of substitution on the targeting behavior","volume":"37","author":"Pezzoli","year":"2017","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Bjorge, J.D., Pang, A., and Fujita, D.J. (2017). Delivery of gene targeting siRNAs to breast cancer cells using a multifunctional peptide complex that promotes both targeted delivery and endosomal release. PLoS ONE.","DOI":"10.1371\/journal.pone.0180578"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2019\/9367845","article-title":"iRGD: A Promising Peptide for Cancer Imaging and a Potential Therapeutic Agent for Various Cancers","volume":"2019","author":"Zuo","year":"2019","journal-title":"J. Oncol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5687","DOI":"10.1038\/s41467-020-19225-7","article-title":"Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy","volume":"11","author":"Gregory","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Egorova, A., Selutin, A., Maretina, M., Selkov, S., Baranov, V., and Kiselev, A. (2020). Characterization of iRGD-ligand modified arginine-histidine-rich peptides for nucleic acid therapeutics delivery to \u03b1v\u03b23 integrin-expressing cancer cells. Pharmaceuticals, 13.","DOI":"10.3390\/ph13100300"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1134\/S0026893320030061","article-title":"Cys-flanked cationic peptides for cell delivery of the herpes simplex virus thymidine kinase gene for suicide gene therapy of uterine leiomyoma","volume":"54","author":"Egorova","year":"2020","journal-title":"Mol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1002\/jgm.2811","article-title":"Development of a receptor-targeted gene delivery system using CXCR4 ligand-conjugated cross-linking peptides","volume":"16","author":"Egorova","year":"2014","journal-title":"J. Gene Med."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Aitken, A., and Learmonth, M. (1996). Estimation of disulfide bonds using Ellman\u2019s reagent. The Protein Protocols Handbook, Humana Press.","DOI":"10.1007\/978-1-60327-259-9_82"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"5397","DOI":"10.1016\/j.biomaterials.2010.03.036","article-title":"Reducing non-specific binding and uptake of nanoparticles and improving cell targeting with an antifouling PEO-b-P\u03b3MPS copolymer coating","volume":"31","author":"Chen","year":"2010","journal-title":"Biomaterials"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.ijpharm.2013.07.069","article-title":"Cytoplasm-responsive nanocarriers conjugated with a functional cell-penetrating peptide for systemic siRNA delivery","volume":"455","author":"Tanaka","year":"2013","journal-title":"Int. J. Pharm."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.ijpharm.2015.05.003","article-title":"Functional peptide nanocarriers for delivery of novel anti-RelA RNA interference agents as a topical treatment of atopic dermatitis","volume":"489","author":"Kanazawa","year":"2015","journal-title":"Int. J. Pharm."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.ijpharm.2016.10.033","article-title":"Systemic delivery of small interfering RNA targeting nuclear factor \u03baB in mice with collagen-induced arthritis using arginine-histidine-cysteine based oligopeptide-modified polymer nanomicelles","volume":"515","author":"Kanazawa","year":"2016","journal-title":"Int. J. Pharm."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.ijpharm.2018.03.026","article-title":"Transdermal anti-nuclear kappaB siRNA therapy for atopic dermatitis using a combination of two kinds of functional oligopeptide","volume":"542","author":"Ibaraki","year":"2018","journal-title":"Int. J. Pharm."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1038\/s41434-018-0042-7","article-title":"Anti-angiogenic treatment of endometriosis via anti-VEGFA siRNA delivery by means of peptide-based carrier in a rat subcutaneous model","volume":"25","author":"Egorova","year":"2018","journal-title":"Gene Ther."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"15307","DOI":"10.1074\/jbc.272.24.15307","article-title":"Binding of cationic \u03b1-helical peptides to plasmid DNA and their gene transfer abilities into cells","volume":"272","author":"Niidome","year":"1997","journal-title":"J. Biol. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1002\/(SICI)1097-0282(1997)44:3<269::AID-BIP6>3.0.CO;2-T","article-title":"DNA condensation by multivalent cations","volume":"44","author":"Bloomfield","year":"1997","journal-title":"Biopolymers"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0168-3659(02)00178-5","article-title":"Structure-activity relationships of poly(l-lysines): Effects of pegylation and molecular shape on physicochemical and biological properties in gene delivery","volume":"83","author":"Vanderkerken","year":"2002","journal-title":"J. Control. Release"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/jgm.522","article-title":"Cell-surface glycosaminoglycans inhibit cation-mediated gene transfer","volume":"6","author":"Ruponen","year":"2004","journal-title":"J. Gene Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jconrel.2008.07.018","article-title":"Glycosaminoglycan-resistant and pH-sensitive lipid-coated DNA complexes produced by detergent removal method","volume":"131","author":"Lehtinen","year":"2008","journal-title":"J. Control. Release"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1002\/jgm.975","article-title":"Interaction of polyamine gene vectors with RNA leads to the dissociation of plasmid DNA-carrier complexes","volume":"8","author":"Huth","year":"2006","journal-title":"J. Gene Med."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Mounkes, L.C., Zhong, W., Cipres-Palacin, G., Heath, T.D., and Debs, R.J. (1998). Proteoglycans mediate cationic liposome-DNA complex-based gene delivery in vitro and in vivo. J. Biol. Chem.","DOI":"10.1074\/jbc.273.40.26164"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.tibtech.2018.02.006","article-title":"Design of polymeric gene carriers for effective intracellular delivery","volume":"36","author":"Lai","year":"2018","journal-title":"Trends Biotechnol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.addr.2019.08.004","article-title":"Brief update on endocytosis of nanomedicines","volume":"144","author":"Patel","year":"2019","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"335","DOI":"10.2174\/156652308786071014","article-title":"Polymer-based gene delivery: A current review on the uptake and intracellular trafficking of polyplexes","volume":"8","author":"Midoux","year":"2008","journal-title":"Curr. Gene Ther."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0167-4781(00)00220-7","article-title":"Polymer chemical structure is a key determinant of physicochemical and colloidal properties of polymer\u2013DNA complexes for gene delivery","volume":"1517","author":"Jones","year":"2000","journal-title":"Biochim. Biophys. Acta Gene Struct. Expr."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"10017","DOI":"10.1021\/acs.langmuir.5b02408","article-title":"Comblike polyethylenimine-based polyplexes: Balancing toxicity, cell internalization, and transfection efficiency via polymer chain topology","volume":"31","author":"Haladjova","year":"2015","journal-title":"Langmuir"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.jconrel.2019.10.046","article-title":"Polymeric siRNA gene delivery\u2014Transfection efficiency versus cytotoxicity","volume":"316","author":"Kargaard","year":"2019","journal-title":"J. Control. Release"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2860","DOI":"10.2174\/138161207782023793","article-title":"Anti-Angiogenesis and RGD-Containing Snake Venom Disintegrins","volume":"13","author":"Swenson","year":"2007","journal-title":"Curr. Pharm. Des."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jconrel.2010.02.002","article-title":"Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles","volume":"149","author":"Vachutinsky","year":"2011","journal-title":"J. Control. Release"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.2147\/IJN.S38378","article-title":"Nanodrugs: Pharmacokinetics and safety","volume":"9","author":"Onoue","year":"2014","journal-title":"Int. J. Nanomed."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1055\/s-0037-1606303","article-title":"New and Emerging Therapies for Uterine Fibroids","volume":"35","author":"Fritton","year":"2017","journal-title":"Semin. Reprod. Med."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Castillo-Rodr\u00edguez, R.A., Arango-Rodr\u00edguez, M.L., Escobedo, L., Hernandez-Baltazar, D., Gompel, A., Forgez, P., and Mart\u00ednez-Fong, D. (2014). Suicide HSVtk gene delivery by neurotensin-polyplex nanoparticles via the bloodstream and GCV treatment specifically inhibit the growth of human MDA-MB-231 triple negative breast cancer tumors xenografted in athymic mice. PLoS ONE.","DOI":"10.1371\/journal.pone.0097151"},{"key":"ref_70","first-page":"1096","article-title":"Suicide Gene Therapy Mediated with Exosomes","volume":"12","author":"Andrea","year":"2020","journal-title":"Cancers"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1016\/0002-9378(93)90293-R","article-title":"Expression of connexin-43 in human myometrium and leiomyoma","volume":"169","author":"Andersen","year":"1993","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/S0165-0173(99)00099-5","article-title":"Gap junctions: The \u201ckiss of death\u201d and the \u201ckiss of life\u201d","volume":"32","author":"Rozental","year":"2000","journal-title":"Brain Res. Rev."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/cgt.2009.64","article-title":"The anti-glioma effect of suicide gene therapy using BMSC expressing HSV\/TK combined with overexpression of Cx43 in glioma cells","volume":"17","author":"Huang","year":"2010","journal-title":"Cancer Gene Ther."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1182\/blood.V84.5.1415.bloodjournal8451415","article-title":"Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis","volume":"84","author":"Koopman","year":"1994","journal-title":"Blood"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.colsurfb.2017.06.022","article-title":"Composite liposome-PEI\/nucleic acid lipopolyplexes for safe and efficient gene delivery and gene knockdown","volume":"158","author":"Pinnapireddy","year":"2017","journal-title":"Colloids Surf. B Biointerfaces"}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/13\/2\/202\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:19:08Z","timestamp":1760159948000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/13\/2\/202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,2]]},"references-count":75,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["pharmaceutics13020202"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics13020202","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,2]]}}}