{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T01:43:24Z","timestamp":1785548604134,"version":"3.56.0"},"reference-count":48,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T00:00:00Z","timestamp":1623110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["150010\/2018-4"],"award-info":[{"award-number":["150010\/2018-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/50928-2"],"award-info":[{"award-number":["2014\/50928-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["23038.000776\/2017O54"],"award-info":[{"award-number":["23038.000776\/2017O54"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CAPES \/ COFECUB","award":["860\/15"],"award-info":[{"award-number":["860\/15"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Since the possibility of silencing specific genes linked to retinal degeneration has become a reality with the use of small interfering RNAs (siRNAs), this technology has been widely studied to promote the treatment of several ocular diseases. Despite recent advances, the clinical success of gene silencing in the retina is significantly reduced by inherent anatomical and physiological ocular barriers, and new strategies are required to achieve intraocular therapeutic effectiveness. In this study, we developed lipoplexes, prepared with sodium alginate as an adjuvant and strategically coated with hyaluronic acid (HA-LIP), and investigated the potential neuroprotective effect of these systems in a retinal light damage model. Successful functionalization of the lipoplexes with hyaluronic acid was indicated in the dynamic light scattering and transmission electron microscopy results. Moreover, these HA-LIP nanoparticles were able to protect and deliver siRNA molecules targeting caspase-3 into the retina. After retinal degeneration induced by high light exposure, in vitro and in vivo quantitative reverse transcription-PCR (RT-qPCR) assays demonstrated significant inhibition of caspase-3 expression by HA-LIP. Furthermore, these systems were shown to be safe, as no evidence of retinal toxicity was observed by electroretinography, clinical evaluation or histology.<\/jats:p>","DOI":"10.3390\/pharmaceutics13060845","type":"journal-article","created":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T21:16:58Z","timestamp":1623187018000},"page":"845","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Neuroprotective Effect of siRNA Entrapped in Hyaluronic Acid-Coated Lipoplexes by Intravitreal Administration"],"prefix":"10.3390","volume":"13","author":[{"given":"Marcela Coelho Silva","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil"},{"name":"Universit\u00e9 de Paris, CNRS, INSERM, UTCBS, Unit\u00e9 des Technologies Chimiques et Biologiques pour la Sant\u00e9, 75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9021-4912","authenticated-orcid":false,"given":"Marcelo Coutinho","family":"de Miranda","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pricila da Silva","family":"Cunha","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9038-1254","authenticated-orcid":false,"given":"Gracielle Ferreira","family":"Andrade","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias da Sa\u00fade, Universidade Federal do Esp\u00edrito Santo, 29932-540 S\u00e3o Mateus, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo de Oliveira","family":"Fulg\u00eancio","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7714-991X","authenticated-orcid":false,"given":"Dawidson Assis","family":"Gomes","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8068-5211","authenticated-orcid":false,"given":"S\u00edlvia Ligorio","family":"Fialho","sequence":"additional","affiliation":[{"name":"Diretoria de Pesquisa e Desenvolvimento, Funda\u00e7\u00e3o Ezequiel Dias, 30510-010 Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederico","family":"Pittella","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Juiz de Fora, 36036-900 Juiz de Fora, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7768-4605","authenticated-orcid":false,"given":"Christine","family":"Charrueau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, CNRS, INSERM, UTCBS, Unit\u00e9 des Technologies Chimiques et Biologiques pour la Sant\u00e9, 75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Escriou","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, CNRS, INSERM, UTCBS, Unit\u00e9 des Technologies Chimiques et Biologiques pour la Sant\u00e9, 75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1161-8936","authenticated-orcid":false,"given":"Armando","family":"Silva-Cunha","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1136\/bjophthalmol-2011-300539","article-title":"Global estimates of visual impairment: 2010","volume":"96","author":"Pascolini","year":"2012","journal-title":"Br. 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