{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T07:32:53Z","timestamp":1785569573540,"version":"3.56.0"},"reference-count":64,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Padova, STARS Starting Grants (STARS StG)","award":["MAST_STARS18_02, CUP C91I18001190005"],"award-info":[{"award-number":["MAST_STARS18_02, CUP C91I18001190005"]}]},{"name":"University of Padova, PRIDJ","award":["MAST_SID17_01, CUP C93C17002300005"],"award-info":[{"award-number":["MAST_SID17_01, CUP C93C17002300005"]}]},{"name":"University of Padova, PRIDJ","award":["GARO_SID19_02"],"award-info":[{"award-number":["GARO_SID19_02"]}]},{"name":"National Science Center, Poland, SONATINA","award":["2019\/32\/C\/NZ7\/00156"],"award-info":[{"award-number":["2019\/32\/C\/NZ7\/00156"]}]},{"name":"National Institute of Public Health \u2013 National Institute of Hygiene, Poland","award":["BW-4\/2020"],"award-info":[{"award-number":["BW-4\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Despite significant advances in chemotherapy, the overall prognosis of hepatocellular carcinoma (HCC) remains extremely poor. HCC targeting strategies were combined with the tumor cell cytotoxicity of oncolytic viruses (OVs) to develop a more efficient and selective therapeutic system. OVs were coated with a polygalactosyl-b-agmatyl diblock copolymer (Gal32-b-Agm29), with high affinity for the asialoglycoprotein receptor (ASGPR) expressed on the liver cell surface, exploiting the electrostatic interaction of the positively charged agmatine block with the negatively charged adenoviral capsid surface. The polymer coating altered the viral particle diameter (from 192 to 287 nm) and zeta-potential (from \u201324.7 to 23.3 mV) while hiding the peculiar icosahedral symmetrical OV structure, as observed by TEM. Coated OVs showed high potential therapeutic value on the human hepatoma cell line HepG2 (cytotoxicity of 72.4% \u00b1 4.96), expressing a high level of ASGPRs, while a lower effect was attained with ASPGR-negative A549 cell line (cytotoxicity of 54.4% \u00b1 1.59). Conversely, naked OVs showed very similar effects in both tested cell lines. Gal32-b-Agm29 OV coating enhanced the infectivity and immunogenic cell death program in HepG2 cells as compared to the naked OV. This strategy provides a rationale for future studies utilizing oncolytic viruses complexed with polymers toward effective treatment of hepatocellular carcinoma.<\/jats:p>","DOI":"10.3390\/pharmaceutics13070949","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T11:01:38Z","timestamp":1624532498000},"page":"949","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Polymer Coated Oncolytic Adenovirus to Selectively Target Hepatocellular Carcinoma Cells"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5001-7826","authenticated-orcid":false,"given":"Mariangela","family":"Garofalo","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Bellato","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Magliocca","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Malfanti","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Catholique de Louvain, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials, Avenue Mounier, 73 bte B1 73.12, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1887-6361","authenticated-orcid":false,"given":"Lukasz","family":"Kuryk","sequence":"additional","affiliation":[{"name":"Department of Virology, National Institute of Public Health\u2014National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland"},{"name":"Clinical Science, Targovax Oy, Saukonpaadenranta 2, 00180 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate","family":"Rinner","sequence":"additional","affiliation":[{"name":"Division of Biomedical Research, Medical University of Graz, Roseggerweg 48, 8036 Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuele","family":"Negro","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58\/B, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4705-5909","authenticated-orcid":false,"given":"Stefano","family":"Salmaso","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Caliceti","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2499-5490","authenticated-orcid":false,"given":"Francesca","family":"Mastrotto","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via F. Marzolo 5, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3322\/CA.2007.0010","article-title":"Cancer Statistics","volume":"58","author":"Jemal","year":"2008","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.bpg.2015.09.007","article-title":"Changing epidemiology of hepatocellular carcinoma in Asia","volume":"29","author":"Goh","year":"2015","journal-title":"Best Pract. Res. Clin. Gastroenterol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1038\/s41575-020-0284-x","article-title":"Polyploidy in liver development, homeostasis and disease","volume":"17","author":"Donne","year":"2020","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1111\/j.1469-0691.2009.03035.x","article-title":"Hepatitis B and C virus-related carcinogenesis","volume":"15","author":"Fung","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3603","DOI":"10.3748\/wjg.v16.i29.3603","article-title":"Non-viral causes of hepatocellular carcinoma","volume":"16","author":"Blonski","year":"2010","journal-title":"World J. Gastroenterol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1038\/onc.2010.36","article-title":"Viral hepatocarcinogenesis","volume":"29","author":"Tsai","year":"2010","journal-title":"Oncogene"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"69","DOI":"10.14218\/JCTH.2017.00031","article-title":"Current and Future Treatment of Hepatocellular Carcinoma: An Updated Comprehensive Review","volume":"6","author":"Daher","year":"2018","journal-title":"J. Clin. Transl. Hepatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ejps.2017.02.016","article-title":"Re-programming pullulan for targeting and controlled release of doxorubicin to the hepatocellular carcinoma cells","volume":"103","author":"Balasso","year":"2017","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"108852","DOI":"10.1016\/j.biopha.2019.108852","article-title":"Current status of nanomaterial-based treatment for hepatocellular carcinoma","volume":"116","author":"Baig","year":"2019","journal-title":"Biomed. Pharm."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/2162402X.2015.1105429","article-title":"Oncolytic adenoviruses coated with MHC-I tumor epitopes increase the antitumor immunity and efficacy against melanoma","volume":"5","author":"Capasso","year":"2016","journal-title":"Oncoimmunology"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Garofalo, M., Villa, A., Rizzi, N., Kuryk, L., Mazzaferro, V., and Ciana, P. (2018). Systemic Administration and Targeted Delivery of Immunogenic Oncolytic Adenovirus Encapsulated in Extracellular Vesicles for Cancer Therapies. Viruses, 10.","DOI":"10.3390\/v10100558"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e1319028","DOI":"10.1080\/2162402X.2017.1319028","article-title":"A novel in silico framework to improve MHC-I epitopes and break the tolerance to melanoma","volume":"6","author":"Capasso","year":"2017","journal-title":"Oncoimmunology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7150\/ntno.47226","article-title":"Cancer-derived EVs show tropism for tissues at early stage of neoplastic transformation","volume":"5","author":"Garofalo","year":"2021","journal-title":"Nanotheranostics"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kuryk, L., Bertinato, L., Staniszewska, M., Pancer, K., Wieczorek, M., Salmaso, S., Caliceti, P., and Garofalo, M. (2020). From Conventional Therapies to Immunotherapy: Melanoma Treatment in Review. Cancers, 12.","DOI":"10.3390\/cancers12103057"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/mt.2009.266","article-title":"Current Issues and Future Directions of Oncolytic Adenoviruses","volume":"18","author":"Yamamoto","year":"2010","journal-title":"Mol. Ther."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jmv.25229","article-title":"Anti-tumor specific T-cell responses induced by oncolytic adenovirus ONCOS-102 in peritoneal mesothelioma mouse model","volume":"90","author":"Kuryk","year":"2018","journal-title":"J. Med. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1002\/jmv.25501","article-title":"Abscopal effect when combining oncolytic adenovirus and checkpoint inhibitor in a humanized NOG mouse model of melanoma","volume":"91","author":"Kuryk","year":"2019","journal-title":"J. Med. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kuryk, L., M\u00f8ller, A.-S., Vuolanto, A., Pesonen, S., Garofalo, M., Cerullo, V., and Jaderberg, M. (2019). Optimization of Early Steps in Oncolytic Adenovirus ONCOS-401 Production in T-175 and HYPERFlasks. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20030621"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Jing, Y., Chavez, V., Khatwani, N., Ban, Y., Espejo, A.P., Chen, X., and Merchan, J.R. (2020). In vivo antitumor activity by dual stromal and tumor-targeted oncolytic measles viruses. Cancer Gene Ther.","DOI":"10.1038\/s41417-020-0171-1"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kuryk, L., Vassilev, L., Ranki, T., Hemminki, A., Karioja-Kallio, A., Levalampi, O., Vuolanto, A., Cerullo, V., and Pesonen, S. (2017). Toxicological and bio-distribution profile of a GM-CSF-expressing, double-targeted, chimeric oncolytic adenovirus ONCOS-102\u2014Support for clinical studies on advanced cancer treatment. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0182715"},{"key":"ref_21","first-page":"1","article-title":"Two roads for oncolytic immunotherapy development","volume":"2","author":"Kaufman","year":"2019","journal-title":"J. Immunother. Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s40257-016-0238-9","article-title":"Talimogene Laherparepvec (T-VEC) and Other Oncolytic Viruses for the Treatment of Melanoma","volume":"18","author":"Bommareddy","year":"2017","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.3389\/fonc.2019.01182","article-title":"Oncolytic Adenovirus-A Nova for Gene-Targeted Oncolytic Viral Therapy in HCC","volume":"9","author":"Abudoureyimu","year":"2019","journal-title":"Front. Oncol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-20213-7","article-title":"Synergistic Anti-tumour Effects of Quercetin and Oncolytic Adenovirus expressing TRAIL in Human Hepatocellular Carcinoma","volume":"8","author":"Zou","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_25","first-page":"1","article-title":"Hepatocellular carcinoma-targeting oncolytic adenovirus overcomes hypoxic tumor microenvironment and effectively disperses through both central and peripheral tumor regions","volume":"8","author":"Yoon","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1089\/hum.2011.003","article-title":"Systemic Delivery of a Novel Liver-Detargeted Oncolytic Adenovirus Causes Reduced Liver Toxicity but Maintains the Antitumor Response in a Breast Cancer Bone Metastasis Model","volume":"22","author":"Zhang","year":"2011","journal-title":"Hum. Gene Ther."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s42490-019-0016-x","article-title":"Overcoming the limitations of locally administered oncolytic virotherapy","volume":"1","author":"Hong","year":"2019","journal-title":"BMC Biomed. Eng."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.jconrel.2015.02.022","article-title":"Asialoglycoprotein receptor mediated hepatocyte targeting\u2014Strategies and applications","volume":"203","author":"Devarajan","year":"2015","journal-title":"J. Control. Release"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3008","DOI":"10.1021\/acs.biomac.5b00906","article-title":"Asialoglycoprotein Receptor-Mediated Gene Delivery to Hepatocytes Using Galactosylated Polymers","volume":"16","author":"Thapa","year":"2015","journal-title":"Biomacromolecules"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1080\/00397919608003754","article-title":"An Improved Procedure for the Large Scale Preparation of 2-Chloro-4,6-dimethoxy-1,3,5-triazine","volume":"26","author":"Cronin","year":"1996","journal-title":"Synth. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"13159","DOI":"10.1016\/S0040-4020(99)00809-1","article-title":"4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride: An efficient condensing agent leading to the formation of amides and esters","volume":"55","author":"Kunishima","year":"1999","journal-title":"Tetrahedron"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1039\/C5PY00166H","article-title":"Rapid synthesis of ultrahigh molecular weight and low polydispersity polystyrene diblock copolymers by RAFT-mediated emulsion polymerization","volume":"6","author":"Truong","year":"2015","journal-title":"Polym. Chem."},{"key":"ref_33","unstructured":"Luisa, M.-P.F.M., and Giuseppe, M. (2018). Sulfated Glycopolymers. (WO\/2018\/007827), Patent."},{"key":"ref_34","unstructured":"Mattias, A.P.B., and Nicolas, T. (2007). Method for Synthesis of Acrylamide Derivatives. (7,294,743), U.S. Patent."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3451","DOI":"10.1021\/ma402435n","article-title":"Pushing the Limit of the RAFT Process: Multiblock Copolymers by One-Pot Rapid Multiple Chain Extensions at Full Monomer Conversion","volume":"47","author":"Gody","year":"2014","journal-title":"Macromolecules"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6714","DOI":"10.1039\/C6PY01292B","article-title":"One-pot RAFT and fast polymersomes assembly: A \u2018beeline\u2019 from monomers to drug-loaded nanovectors","volume":"7","author":"Mastrotto","year":"2016","journal-title":"Polym. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/S1525-0016(03)00200-4","article-title":"Enhanced therapeutic efficacy for ovarian cancer with a serotype 3 receptor-targeted oncolytic adenovirus","volume":"8","author":"Kanerva","year":"2003","journal-title":"Mol. Ther."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1002\/ijc.20129","article-title":"Modified adenoviruses for cancer gene therapy","volume":"110","author":"Kanerva","year":"2004","journal-title":"Int. J. Cancer"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/s41417-018-0038-x","article-title":"Quantification and functional evaluation of CD40L production from the adenovirus vector ONCOS-401","volume":"26","author":"Kuryk","year":"2019","journal-title":"Cancer Gene Ther."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Garofalo, M., Bertinato, L., Staniszewska, M., Wieczorek, M., Salmaso, S., Schrom, S., Rinner, B., Pancer, K.W., and Kuryk, L. (2021). Combination Therapy of Novel Oncolytic Adenovirus with Anti-PD1 Resulted in Enhanced Anti-Cancer Effect in Syngeneic Immunocompetent Melanoma Mouse Model. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13040547"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1038\/mt.2010.161","article-title":"Treatment of cancer patients with a serotype 5\/3 chimeric oncolytic adenovirus expressing GMCSF","volume":"18","author":"Koski","year":"2010","journal-title":"Mol. Ther. J. Am. Soc. Gene Ther."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1002\/jmv.25677","article-title":"Chimeric oncolytic Ad5\/3 virus replicates and lyses ovarian cancer cells through desmoglein-2 cell entry receptor","volume":"92","author":"Kuryk","year":"2020","journal-title":"J. Med. Virol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1158\/1535-7163.MCT-15-0559","article-title":"Oncolytic Adenovirus Loaded with L-carnosine as Novel Strategy to Enhance the Antitumor Activity","volume":"15","author":"Garofalo","year":"2016","journal-title":"Mol. Cancer Ther."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e01071","DOI":"10.1016\/j.heliyon.2018.e01071","article-title":"Development of asialoglycoprotein receptor directed nanoparticles for selective delivery of curcumin derivative to hepatocellular carcinoma","volume":"4","author":"Yousef","year":"2018","journal-title":"Heliyon"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5095","DOI":"10.1016\/S0021-9258(18)32543-2","article-title":"The Large Intracellular Pool of Asialoglycoprotein Receptors Functions during the Endocytosis of Asialoglycoproteins by Isolated Rat Hepatocytes","volume":"258","author":"Weigels","year":"1983","journal-title":"J. Biol. Chem."},{"key":"ref_46","first-page":"41","article-title":"Oligosaccharide Valency and Conformation in Determining Binding to the Asialoglycoprotein Receptor of Rat Hepatocytes","volume":"66","author":"Rice","year":"1993","journal-title":"Adv. Enzymol. Relat. Areas Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/s11095-006-9574-4","article-title":"Molar-Mass Characterization of Cationic Polymers for Gene Delivery by Aqueous Size-Exclusion Chromatography","volume":"23","author":"Jiang","year":"2006","journal-title":"Pharm. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.ajps.2016.11.001","article-title":"Molecular weight determination of a newly synthesized guanidinylated disulfide-containing poly(amido amine) by gel permeation chromatography","volume":"12","author":"Xing","year":"2017","journal-title":"Asian J. Pharm. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6479","DOI":"10.1074\/jbc.272.10.6479","article-title":"Complexes of Adenovirus with Polycationic Polymers and Cationic Lipids Increase the Efficiency of Gene Transfer in Vitro and in Vivo","volume":"272","author":"Fasbender","year":"1997","journal-title":"J. Biol. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/sj.gt.3301389","article-title":"Polymer-coated adenovirus permits efficient retargeting and evades neutralising antibodies","volume":"8","author":"Fisher","year":"2001","journal-title":"Gene Ther."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Nikitin, N., Trifonova, E., Evtushenko, E., Kirpichnikov, M., Atabekov, J., and Karpova, O. (2015). Comparative Study of Non-Enveloped Icosahedral Viruses Size. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0142415"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"283974","DOI":"10.1155\/2012\/283974","article-title":"ASGR1 and ASGR2, the Genes that Encode the Asialoglycoprotein Receptor (Ashwell Receptor), Are Expressed in Peripheral Blood Monocytes and Show Interindividual Differences in Transcript Profile","volume":"2012","author":"Harris","year":"2012","journal-title":"Mol. Biol. Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1038\/sj.cgt.7700985","article-title":"Inhibition of hepatocarcinoma by systemic delivery of Apoptin gene via the hepatic asialoglycoprotein receptor","volume":"14","author":"Peng","year":"2007","journal-title":"Cancer Gene Ther."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"9183","DOI":"10.1128\/JVI.77.17.9183-9191.2003","article-title":"Adenovirus Type 11 Uses CD46 as a Cellular Receptor","volume":"77","author":"Segerman","year":"2003","journal-title":"J. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s00430-019-00645-2","article-title":"Human adenovirus binding to host cell receptors: A structural view","volume":"209","author":"Stasiak","year":"2020","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.omtm.2020.06.010","article-title":"Transient Chimeric Ad5\/37 Fiber Enhances NK-92 Carrier Cell-Mediated Delivery of Oncolytic Adenovirus Type 5 to Tumor Cells","volume":"18","author":"Gao","year":"2020","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1021\/bc200666u","article-title":"Star-Like Oligo-Arginyl-Maltotriosyl Derivatives as Novel Cell-Penetrating Enhancers for the Intracellular Delivery of Colloidal Therapeutic Systems","volume":"23","author":"Bersani","year":"2012","journal-title":"Bioconjugate Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e955691","DOI":"10.4161\/21624011.2014.955691","article-title":"Consensus guidelines for the detection of immunogenic cell death","volume":"3","author":"Kepp","year":"2014","journal-title":"OncoImmunology"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/mt.2013.220","article-title":"Oncolytic Virotherapy and Immunogenic Cancer Cell Death: Sharpening the Sword for Improved Cancer Treatment Strategies","volume":"22","author":"Workenhe","year":"2014","journal-title":"Mol. Ther."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Garofalo, M., Staniszewska, M., Salmaso, S., Caliceti, P., Pancer, K.W., Wieczorek, M., and Kuryk, L. (2020). Prospects of Replication-Deficient Adenovirus Based Vaccine Development against SARS-CoV-2. Vaccines, 8.","DOI":"10.3390\/vaccines8020293"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1002\/ijc.30228","article-title":"Synergistic anti-tumor efficacy of immunogenic adenovirus ONCOS-102 (Ad5\/3-D24-GM-CSF) and standard of care chemotherapy in preclinical mesothelioma model","volume":"139","author":"Kuryk","year":"2016","journal-title":"Int. J. Cancer"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e1532763","DOI":"10.1080\/2162402X.2018.1532763","article-title":"Combination of immunogenic oncolytic adenovirus ONCOS-102 with anti-PD-1 pembrolizumab exhibits synergistic antitumor effect in humanized A2058 melanoma huNOG mouse model","volume":"8","author":"Kuryk","year":"2019","journal-title":"Oncoimmunology"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1146\/annurev-immunol-032712-100008","article-title":"Immunogenic Cell Death in Cancer Therapy","volume":"31","author":"Kroemer","year":"2013","journal-title":"Annu. Rev. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1002\/jcb.24012","article-title":"Intratumoral injection of taxol in vivo suppresses A549 tumor showing cytoplasmic vacuolization","volume":"113","author":"Wang","year":"2012","journal-title":"J. Cell. Biochem."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/13\/7\/949\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:23:10Z","timestamp":1760163790000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/13\/7\/949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,24]]},"references-count":64,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2021,7]]}},"alternative-id":["pharmaceutics13070949"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics13070949","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,24]]}}}