{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:09:46Z","timestamp":1787065786211,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T00:00:00Z","timestamp":1625011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["#"],"award-info":[{"award-number":["#"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["# 2018\/23015-7 and  # 2018\/09088-1"],"award-info":[{"award-number":["# 2018\/23015-7 and  # 2018\/09088-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (&gt;9 log10 CFU\u00b7g\u22121), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.<\/jats:p>","DOI":"10.3390\/pharmaceutics13070998","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T02:44:39Z","timestamp":1625107479000},"page":"998","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro"],"prefix":"10.3390","volume":"13","author":[{"given":"Virg\u00ednia Barreto","family":"Lordello","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Rodovia Araraquara-Ja\u00fa, Km 01-s\/n, Campus Ville, Araraquara 14800-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9ia Bagliotti","family":"Meneguin","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Rodovia Araraquara-Ja\u00fa, Km 01-s\/n, Campus Ville, Araraquara 14800-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Raquel","family":"de Annunzio","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Rodovia Araraquara-Ja\u00fa, Km 01-s\/n, Campus Ville, Araraquara 14800-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria P\u00eda","family":"Taranto","sequence":"additional","affiliation":[{"name":"Reference Center for Lactobacilli (CERELA-CONICET), San Miguel de Tucum\u00e1n, Chacabuco 145, Tucum\u00e1n T4000 ILC, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6698-0545","authenticated-orcid":false,"given":"Marlus","family":"Chorilli","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Rodovia Araraquara-Ja\u00fa, Km 01-s\/n, Campus Ville, Araraquara 14800-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9135-3690","authenticated-orcid":false,"given":"Carla Raquel","family":"Fontana","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Rodovia Araraquara-Ja\u00fa, Km 01-s\/n, Campus Ville, Araraquara 14800-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2146-662X","authenticated-orcid":false,"given":"Daniela Cardoso Umbelino","family":"Cavallini","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), Rodovia Araraquara-Ja\u00fa, Km 01-s\/n, Campus Ville, Araraquara 14800-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,30]]},"reference":[{"key":"ref_1","unstructured":"FAO (Food and Agricultural Organization of the United Nations), and World Health Organization (2021, March 19). 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