{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:43:07Z","timestamp":1760366587803,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T00:00:00Z","timestamp":1641254400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Microvesicles, so-called endothelial large extracellular vesicles (LEVs), are of great interest as biological markers and cell-free biotherapies in cardiovascular and oncologic diseases. However, their therapeutic perspectives remain limited due to the lack of reliable data regarding their systemic biodistribution after intravenous administration. Methods: Applied to a mouse model of peripheral ischemia, radiolabeled endothelial LEVs were tracked and their in vivo whole-body distribution was quantified by microSPECT\/CT imaging. Hindlimb perfusion was followed by LASER Doppler and motility impairment function was evaluated up to day 28 post-ischemia. Results: Early and specific homing of LEVs to ischemic hind limbs was quantified on the day of ischemia and positively correlated with reperfusion intensity at a later stage on day 28 after ischemia, associated with an improved motility function. Conclusions: This concept is a major asset for investigating the biodistribution of LEVs issued from other cell types, including cancer, thus partly contributing to better knowledge and understanding of their fate after injection.<\/jats:p>","DOI":"10.3390\/pharmaceutics14010121","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T20:21:56Z","timestamp":1641759716000},"page":"121","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT\/CT Imaging"],"prefix":"10.3390","volume":"14","author":[{"given":"Romain","family":"Giraud","sequence":"first","affiliation":[{"name":"C2VN, INSERM, INRAE, Aix Marseille University, 13385 Marseille, France"},{"name":"CERIMED, CNRS, Marseille, Aix Marseille University, 13385 Marseille, France"},{"name":"Radiopharmacy, P\u00f4le Pharmacie, University Hospitals of Marseille, APHM, 13005 Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6668-6031","authenticated-orcid":false,"given":"Ana\u00efs","family":"Moyon","sequence":"additional","affiliation":[{"name":"C2VN, INSERM, INRAE, Aix Marseille University, 13385 Marseille, France"},{"name":"CERIMED, CNRS, Marseille, Aix Marseille University, 13385 Marseille, France"},{"name":"Radiopharmacy, P\u00f4le Pharmacie, University Hospitals of Marseille, APHM, 13005 Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phanie","family":"Simoncini","sequence":"additional","affiliation":[{"name":"C2VN, INSERM, INRAE, Aix Marseille University, 13385 Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne-Claire","family":"Duchez","sequence":"additional","affiliation":[{"name":"C2VN, INSERM, INRAE, Aix 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