{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:09:16Z","timestamp":1787501356688,"version":"build-2736575974"},"reference-count":85,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T00:00:00Z","timestamp":1642032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005669","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Estado da Para\u00edba","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100005669","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>The compound (E)-2-cyano-N,3-diphenylacrylamide (JMPR-01) was structurally developed using bioisosteric modifications of a hybrid prototype as formed from fragments of indomethacin and paracetamol. Initially, in vitro assays were performed to determine cell viability (in macrophage cultures), and its ability to modulate the synthesis of nitrite and cytokines (IL-1\u03b2 and TNF\u03b1) in non-cytotoxic concentrations. In vivo, anti-inflammatory activity was explored using the CFA-induced paw edema and zymosan-induced peritonitis models. To investigate possible molecular targets, molecular docking was performed with the following crystallographic structures: LT-A4-H, PDE4B, COX-2, 5-LOX, and iNOS. As results, we observed a significant reduction in the production of nitrite and IL-1\u03b2 at all concentrations used, and also for TNF\u03b1 with JMPR-01 at 50 and 25 \u03bcM. The anti-edematogenic activity of JMPR-01 (100 mg\/kg) was significant, reducing edema at 2\u20136 h, similar to the dexamethasone control. In induced peritonitis, JMPR-01 reduced leukocyte migration by 61.8, 68.5, and 90.5% at respective doses of 5, 10, and 50 mg\/kg. In silico, JMPR-01 presented satisfactory coupling; mainly with LT-A4-H, PDE4B, and iNOS. These preliminary results demonstrate the strong potential of JMPR-01 to become a drug for the treatment of inflammatory diseases.<\/jats:p>","DOI":"10.3390\/pharmaceutics14010188","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T03:14:03Z","timestamp":1642130043000},"page":"188","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Compound (E)-2-Cyano-N,3-diphenylacrylamide (JMPR-01): A Potential Drug for Treatment of Inflammatory Diseases"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8354-3198","authenticated-orcid":false,"given":"Pablo Rayff","family":"da Silva","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"},{"name":"Laborat\u00f3rio de Ensaios Farmacol\u00f3gicos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"},{"name":"Laborat\u00f3rio de Desenvolvimento e S\u00edntese de F\u00e1rmacos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renan Fernandes","family":"do Esp\u00edrito Santo","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Osvaldo Cruz, Salvador 40296-710, BA, Brazil"},{"name":"Faculdade de Farm\u00e1cia, Universidade Federal da Bahia, Salvador 40170-290, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7256-8891","authenticated-orcid":false,"given":"Camila de Oliveira","family":"Melo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"},{"name":"Laborat\u00f3rio de Desenvolvimento e S\u00edntese de F\u00e1rmacos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5870-464X","authenticated-orcid":false,"given":"F\u00e1bio Emanuel","family":"Pach\u00fa Cavalcante","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ensaios Farmacol\u00f3gicos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Th\u00e1ssia Borges","family":"Costa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ensaios Farmacol\u00f3gicos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmim Vilarim","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ensaios Farmacol\u00f3gicos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvnni M. S. de Medeiros","family":"e Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento e S\u00edntese de F\u00e1rmacos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e1lia Ferreira","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Produtos Naturais, Sint\u00e9ticos e Bioativos, Universidade Federal da Paraiba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0113-7864","authenticated-orcid":false,"given":"Cristiane Flora","family":"Villarreal","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz, Funda\u00e7\u00e3o Osvaldo Cruz, Salvador 40296-710, BA, Brazil"},{"name":"Faculdade de Farm\u00e1cia, Universidade Federal da Bahia, Salvador 40170-290, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1590-3750","authenticated-orcid":false,"given":"Ricardo Ol\u00edmpio","family":"de Moura","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"},{"name":"Laborat\u00f3rio de Desenvolvimento e S\u00edntese de F\u00e1rmacos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6083-5390","authenticated-orcid":false,"given":"Vanda Lucia","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"},{"name":"Laborat\u00f3rio de Ensaios Farmacol\u00f3gicos, Departamento de Farm\u00e1cia, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,13]]},"reference":[{"key":"ref_1","first-page":"PA1588","article-title":"Investigation of CD14 with toll like receptors expression in pleural effusion for detection of thoracic disorders","volume":"50","author":"Mortaz","year":"2017","journal-title":"Eur. 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