{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T21:30:56Z","timestamp":1773696656627,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["308755\/2018-9"],"award-info":[{"award-number":["308755\/2018-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/203.246\/2017"],"award-info":[{"award-number":["E-26\/203.246\/2017"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/211.025\/2019"],"award-info":[{"award-number":["E-26\/211.025\/2019"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/200.982\/2021"],"award-info":[{"award-number":["E-26\/200.982\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>P2X7R is a purinergic receptor with broad expression throughout the body, especially in immune system cells. P2X7R activation causes inflammatory mediators to release, including interleukin-1\u03b2 (IL-1\u03b2), the processing and release of which are critically dependent on this ion channel activation. P2X7R\u2019s therapeutic potential augments the discovery of new antagonistic compounds. Thus, we investigated whether the Eugenia sulcata essential oil could block P2X7R activity. The essential oil (ESO) dose-dependently inhibited ATP-promoted PI uptake and IL-1\u03b2 release with an IC50 of 113.3 \u00b1 3.7 ng\/mL and 274 \u00b1 91 ng\/mL, respectively, and the essential oil nanoemulsion (ESON) improved the ESO inhibitory effect with an IC50 of 81.4 \u00b1 7.2 ng\/mL and 62 \u00b1 2 ng\/mL, respectively. ESO and ESON reversed the carrageenan-activated peritonitis in mice, and ESON exhibited an efficacy higher than ESO. The majority substance from essential oil, \u03b2-caryophyllene, impaired the ATP-evoked PI uptake and IL-1\u03b2 release with an IC50 value of 26 \u00b1 0.007 ng\/mL and 97 \u00b1 0.012 ng\/mL, respectively. Additionally, \u03b2-caryophyllene reduced carrageenan-induced peritonitis, and the molecular modeling and computational simulation predicted the intermolecular interactions in the P2X7R situs. In silico, results indicated \u03b2-caryophyllene as a potent allosteric P2X7R antagonist, although this substance may present toxic effects for humans. These data confirm the nanoemulsion of essential oil from E. sulcata as a promisor biotechnology strategy for impaired P2X7R functions and the inflammatory response.<\/jats:p>","DOI":"10.3390\/pharmaceutics14050911","type":"journal-article","created":{"date-parts":[[2022,4,24]],"date-time":"2022-04-24T00:45:21Z","timestamp":1650761121000},"page":"911","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Eugenia sulcata (Myrtaceae) Nanoemulsion Enhances the Inhibitory Activity of the Essential Oil on P2X7R and Inflammatory Response In Vivo"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8521-5171","authenticated-orcid":false,"given":"Bettina Quintanilha","family":"Magalh\u00e3es","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia, Alimenta\u00e7\u00e3o e Sa\u00fade, Universidade Federal Fluminense, R. Alexandre Moura, 8-S\u00e3o Domingos, Niter\u00f3i 24210-200, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5332-9972","authenticated-orcid":false,"given":"Francisco P.","family":"Machado","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia de Produtos Naturais, Faculdade de Farm\u00e1cia, Universidade Federal Fluminense, Rua Doutor M\u00e1rio Viana 523, Santa Rosa, Niter\u00f3i 24241-000, Brazil"},{"name":"Post-Graduation Program in Vegetal Biotechnology and Bioprocesses, Rio de Janeiro Federal University, Avenida Carlos Chagas Filho, 373, Cidade Universit\u00e1ria, Niter\u00f3i 21941-599, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola S.","family":"Sanches","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia de Produtos Naturais, Faculdade de Farm\u00e1cia, Universidade Federal Fluminense, Rua Doutor M\u00e1rio Viana 523, Santa Rosa, Niter\u00f3i 24241-000, Brazil"},{"name":"Post-Graduation Program in Vegetal Biotechnology and Bioprocesses, Rio de Janeiro Federal University, Avenida Carlos Chagas Filho, 373, Cidade Universit\u00e1ria, Niter\u00f3i 21941-599, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara","family":"Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia de Produtos Naturais, Faculdade de Farm\u00e1cia, Universidade Federal Fluminense, Rua Doutor M\u00e1rio Viana 523, Santa Rosa, Niter\u00f3i 24241-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah Quintanilha","family":"Falc\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia Farmac\u00eautica I, Faculdade de Farm\u00e1cia, Universidade Federal Fluminense, Rua Doutor M\u00e1rio Viana 523, Santa Rosa, Niter\u00f3i 24241-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"von Ranke","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murilo Lamim","family":"Bello","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Rangel","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Guerra","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias, Faculdade de Forma\u00e7\u00e3o de Professores, Universidade do Estado do Rio de Janeiro, Francisco Portela 1470, S\u00e3o Gon\u00e7alo 24435-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0484-1918","authenticated-orcid":false,"given":"Leandro","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia de Produtos Naturais, Faculdade de Farm\u00e1cia, Universidade Federal Fluminense, Rua Doutor M\u00e1rio Viana 523, Santa Rosa, Niter\u00f3i 24241-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8218-834X","authenticated-orcid":false,"given":"Robson X.","family":"Faria","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Avalia\u00e7\u00e3o e Promo\u00e7\u00e3o da Sa\u00fade Ambiental, Instituto Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro 21040-900, Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias e Biotecnologia, Universidade Federal Fluminense, R. Alexandre Moura, 8-S\u00e3o Domingos, Niter\u00f3i 24210-200, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"key":"ref_1","unstructured":"Judd, W.S., Campbel, C.S., Kellogg, E.A., Stevens, P.F., and Donoghue, M.J. (2009). Sistem\u00e1tica Vegetal\u2014Um Enfoque Filogen\u00e9tico, Artmed Porto Alegre. [3rd ed.]."},{"key":"ref_2","unstructured":"Sobral, M., Proen\u00e7a, C., Souza, M., Mazine, F., and Lucas, E. (2010, October 10). Myrtaceae in Lista de Esp\u00e9cies da Flora do Brasil. Jardim Bot\u00e2nico do Rio de Janeiro. 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