{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:08:50Z","timestamp":1780668530785,"version":"3.54.1"},"reference-count":55,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T00:00:00Z","timestamp":1652745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FAPEMIG","award":["PPM 00296\/16"],"award-info":[{"award-number":["PPM 00296\/16"]}]},{"name":"FAPEMIG","award":["APQ 02015\/14"],"award-info":[{"award-number":["APQ 02015\/14"]}]},{"name":"FAPEMIG","award":["309603\/2021-8"],"award-info":[{"award-number":["309603\/2021-8"]}]},{"name":"FAPEMIG","award":["2020\/01441-4"],"award-info":[{"award-number":["2020\/01441-4"]}]},{"name":"CNPq","award":["PPM 00296\/16"],"award-info":[{"award-number":["PPM 00296\/16"]}]},{"name":"CNPq","award":["APQ 02015\/14"],"award-info":[{"award-number":["APQ 02015\/14"]}]},{"name":"CNPq","award":["309603\/2021-8"],"award-info":[{"award-number":["309603\/2021-8"]}]},{"name":"CNPq","award":["2020\/01441-4"],"award-info":[{"award-number":["2020\/01441-4"]}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["PPM 00296\/16"],"award-info":[{"award-number":["PPM 00296\/16"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["APQ 02015\/14"],"award-info":[{"award-number":["APQ 02015\/14"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["309603\/2021-8"],"award-info":[{"award-number":["309603\/2021-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2020\/01441-4"],"award-info":[{"award-number":["2020\/01441-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Schistosomiasis, caused by parasites of the genus Schistosoma, is a neglected disease with high global prevalence, affecting more than 240 million people in several countries. Praziquantel (PZQ) is the only drug currently available for the treatment. S. mansoni NTPDases (known as SmNTPDases, ATP diphosphohydrolases or ecto-apyrases) are potential drug targets for the discovery of new antischistosomal drugs. In this study, we screen NTPDases inhibitors from Centella erecta (Apiaceae) using an ultrafiltration combined UHPLC-QTOF-MS method and potato apyrase, identifying asiaticoside as one of the apyrase-binding compounds. After isolation of asiaticoside from C. erecta extract, we assessed its in vivo antischistosomal activities against Schistosoma mansoni worms and its in vitro enzymatic apyrase inhibition. Also, molecular docking analysis of asiaticoside against potato apyrase, S. mansoni NTPDases 1 and 2 were performed. Asiaticoside showed a significant in vitro apyrase inhibition and molecular docking studies corroborate with its possible actions in potato apyrase and S. mansoni NTPDases. In mice harboring a patent S. mansoni infection, a single oral dose of asiaticoside (400 mg\/kg. p.o.) showed significantly in vivo antischistosomal efficacy, markedly decreasing the total worm load and egg burden, giving support for further exploration of apyrase inhibitors as antischistosomal agents.<\/jats:p>","DOI":"10.3390\/pharmaceutics14051071","type":"journal-article","created":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T08:34:29Z","timestamp":1652776469000},"page":"1071","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Identification of Asiaticoside from Centella erecta (Apiaceae) as Potential Apyrase Inhibitor by UF-UHPLC-MS and Its In Vivo Antischistosomal Activity"],"prefix":"10.3390","volume":"14","author":[{"given":"Lara Soares Aleixo","family":"de Carvalho","sequence":"first","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Departamento de Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Juiz de Fora, R. Jos\u00e9 Louren\u00e7o Kelmer s\/n, Campus Universit\u00e1rio, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8075-3253","authenticated-orcid":false,"given":"Vin\u00edcius Carius","family":"de Souza","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Modelagem Computacional, Departamento de Ci\u00eancia da Computa\u00e7\u00e3o, Instituto de Ci\u00eancias Exatas, Universidade Federal de Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vin\u00edcius C.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa em Doen\u00e7as Negligenciadas, Universidade Guarulhos, Guarulhos 07023-070, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6948-331X","authenticated-orcid":false,"given":"Aline Correa","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0334-0217","authenticated-orcid":false,"given":"Jorge Willian Leandro","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila V. S. Z.","family":"Capriles","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Modelagem Computacional, Departamento de Ci\u00eancia da Computa\u00e7\u00e3o, Instituto de Ci\u00eancias Exatas, Universidade Federal de Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila de F.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1766-7031","authenticated-orcid":false,"given":"Josu\u00e9","family":"de Moraes","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa em Doen\u00e7as Negligenciadas, Universidade Guarulhos, Guarulhos 07023-070, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9096-1336","authenticated-orcid":false,"given":"Ademar Alves","family":"da Silva Filho","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Departamento de Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Juiz de Fora, R. Jos\u00e9 Louren\u00e7o Kelmer s\/n, Campus Universit\u00e1rio, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.4155\/fmc-2017-0112","article-title":"Antischistosomal agents: State of art and perspectives","volume":"10","author":"Lago","year":"2018","journal-title":"Future Med. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"23437","DOI":"10.1038\/s41598-021-02792-0","article-title":"Pyrazoline derivatives as promising novel antischistosomal agents","volume":"11","author":"Morais","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Porto, R., Mengarda, A.C., Cajas, R.A., Salvadori, M.C., Teixeira, F.S., Arcanjo, D.D.R., Siyadatpanah, A., Pereira, M.L., Wilairatana, P., and Moraes, J. (2021). 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