{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:06:11Z","timestamp":1791237971016,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T00:00:00Z","timestamp":1654560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"Ministry of Education of the Republic of Korea and the National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2020R1C1C1005293"],"award-info":[{"award-number":["NRF-2020R1C1C1005293"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Education of the Republic of Korea and the National Research Foundation of Korea","doi-asserted-by":"publisher","award":["HR16C0002"],"award-info":[{"award-number":["HR16C0002"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"South Korean government (MSIT) and the Ministry of Health &amp; Welfare, Republic of Korea","award":["NRF-2020R1C1C1005293"],"award-info":[{"award-number":["NRF-2020R1C1C1005293"]}]},{"name":"South Korean government (MSIT) and the Ministry of Health &amp; Welfare, Republic of Korea","award":["HR16C0002"],"award-info":[{"award-number":["HR16C0002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Recently, various types of in vitro-reconstructed 3D skin models have been developed for drug testing and disease modeling. Herein, we structurally and functionally validated a self-assembled reconstructed skin equivalent (RSE) and developed an IL-17a-induced in vitro psoriasis-like model using a self-assembled RSE. The tissue engineering approach was used to construct the self-assembled RSE. The dermal layer was generated using fibroblasts secreting their own ECM, and the epidermal layer was reconstructed by seeding keratinocytes on the dermal layer. To generate the psoriatic model, IL-17A was added to the culture medium during the air\u2013liquid interface culture period. Self-assembled RSE resulted in a fully differentiated epidermal layer, a well-established basement membrane, and dermal collagen deposition. In addition, self-assembled RSE was tested for 20 reference chemicals according to the Performance Standard of OECD TG439 and showed overall sensitivity, specificity, and accuracy of 100%, 90%, and 95%, respectively. The IL-17a-treated psoriatic RSE model exhibited psoriatic epidermal characteristics, such as epidermal hyperproliferation, parakeratosis, and increased expression of KRT6, KRT17, hBD2, and S100A9. Thus, our results suggest that a self-assembled RSE that structurally and functionally mimics the human skin has a great potential for testing various drugs or cosmetic ingredients and modeling inflammatory skin diseases.<\/jats:p>","DOI":"10.3390\/pharmaceutics14061211","type":"journal-article","created":{"date-parts":[[2022,6,12]],"date-time":"2022-06-12T23:55:24Z","timestamp":1655078124000},"page":"1211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Structural and Functional Validation of a Full-Thickness Self-Assembled Skin Equivalent for Disease Modeling"],"prefix":"10.3390","volume":"14","author":[{"given":"Bo Ram","family":"Mok","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, CHA University, Seongnam 13488, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Ji","family":"Shon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, CHA University, Seongnam 13488, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5458-1985","authenticated-orcid":false,"given":"A Ram","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, CHA University, Seongnam 13488, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyne","family":"Simard-Bisson","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, and Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, Qu\u00e9bec, QC G1J1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isra\u00ebl","family":"Martel","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, and Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, Qu\u00e9bec, QC G1J1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8883-6491","authenticated-orcid":false,"given":"Lucie","family":"Germain","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, and Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, Qu\u00e9bec, QC G1J1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Dermatology, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam 13497, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung U","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Dermatology, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam 13497, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1111\/exd.13531","article-title":"3D skin models for 3R research: The potential of 3D reconstructed skin models to study skin barrier function","volume":"27","author":"Niehues","year":"2018","journal-title":"Exp. 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