{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:03:04Z","timestamp":1762254184121,"version":"build-2065373602"},"reference-count":54,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH\u2014CEBRA PAR","award":["1R21DA043959-01"],"award-info":[{"award-number":["1R21DA043959-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Recent research points to mesenchymal stem cells\u2019 potential for treating neurological disorders, especially drug addiction. We examined the longitudinal effect of placenta-derived mesenchymal stromal-like cells (PLX-PAD) in a rat model for cocaine addiction. Sprague\u2013Dawley male rats were trained to self-administer cocaine or saline daily until stable maintenance. Before the extinction phase, PLX-PAD cells were administered by intracerebroventricular or intranasal routes. Neurogenesis was evaluated, as was behavioral monitoring for craving. We labeled the PLX-PAD cells with gold nanoparticles and followed their longitudinal migration in the brain parallel to their infiltration of essential peripheral organs both by micro-CT and by inductively coupled plasma-optical emission spectrometry. Cell locations in the brain were confirmed by immunohistochemistry. We found that PLX-PAD cells attenuated cocaine-seeking behavior through their capacity to migrate to specific mesolimbic regions, homed on the parenchyma in the dentate gyrus of the hippocampus, and restored neurogenesis. We believe that intranasal cell therapy is a safe and effective approach to treating addiction and may offer a novel and efficient approach to rehabilitation.<\/jats:p>","DOI":"10.3390\/pharmaceutics14071311","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T04:12:01Z","timestamp":1655871121000},"page":"1311","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Placenta-Derived Mesenchymal-like Adherent Stromal Cells as an Effective Cell Therapy for Cocaine Addiction in a Rat Model"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6878-1902","authenticated-orcid":false,"given":"Hilla","family":"Pe\u2019er-Nissan","sequence":"first","affiliation":[{"name":"Neuropharmacology Laboratory, The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadas","family":"Ahdoot-Levi","sequence":"additional","affiliation":[{"name":"Neuropharmacology Laboratory, The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4429-7024","authenticated-orcid":false,"given":"Oshra","family":"Betzer","sequence":"additional","affiliation":[{"name":"The Leslie and Susan Gonda (Goldschmied) Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan 5290002, Israel"},{"name":"Faculty of Engineering & The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pnina Shirel","family":"Itzhak","sequence":"additional","affiliation":[{"name":"Neuropharmacology Laboratory, The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niva","family":"Shraga-Heled","sequence":"additional","affiliation":[{"name":"Pluristem Therapeutics Inc., Haifa 3508409, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris","family":"Gispan","sequence":"additional","affiliation":[{"name":"Neuropharmacology Laboratory, The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel"},{"name":"The Leslie and Susan Gonda (Goldschmied) Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Menachem","family":"Motiei","sequence":"additional","affiliation":[{"name":"Faculty of Engineering & The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur","family":"Doroshev","sequence":"additional","affiliation":[{"name":"The Department of Chemical Engineering, Biotechnology and Materials Ariel University, Ariel 40700, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4747-0774","authenticated-orcid":false,"given":"Yaakov","family":"Anker","sequence":"additional","affiliation":[{"name":"The Department of Chemical Engineering, Biotechnology and Materials Ariel University, Ariel 40700, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachela","family":"Popovtzer","sequence":"additional","affiliation":[{"name":"Faculty of Engineering & The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4417-7460","authenticated-orcid":false,"given":"Racheli","family":"Ofir","sequence":"additional","affiliation":[{"name":"Pluristem Therapeutics Inc., Haifa 3508409, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gal","family":"Yadid","sequence":"additional","affiliation":[{"name":"Neuropharmacology Laboratory, The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel"},{"name":"The Leslie and Susan Gonda (Goldschmied) Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0165-0173(01)00088-1","article-title":"The Neuropsychological Basis of Addictive Behaviour","volume":"36","author":"Everitt","year":"2001","journal-title":"Brain Res. 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