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The safety and the effectiveness of phages have been mainly confirmed in the food industry as well as in animal models. In this study, we report on the successful isolation of phages specific to Vancomycin-resistant Enterococci, including Enterococcus faecium (VREfm) and Enterococcus faecalis from sewage samples, and demonstrate their efficacy and safety for VREfm infection in the greater wax moth Galleria mellonella model. No virulence-associated genes, antibiotic resistance genes or integrases were detected in the phages\u2019 genomes, rendering them safe to be used in an in vivo model. Phages may be considered as potential agents for therapy for bacterial infections secondary to multidrug-resistant organisms such as VREfm.<\/jats:p>","DOI":"10.3390\/pharmaceutics14081591","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T04:04:00Z","timestamp":1659326640000},"page":"1591","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Genomic and Functional Characterization of Vancomycin-Resistant Enterococci-Specific Bacteriophages in the Galleria mellonella Wax Moth Larvae Model"],"prefix":"10.3390","volume":"14","author":[{"given":"Lynn","family":"El Haddad","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Angelidakis","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1590-6828","authenticated-orcid":false,"given":"Justin R.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus F.","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austen L.","family":"Terwilliger","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P.","family":"Chaftari","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Duna","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena T.","family":"Yusuf","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia P.","family":"Harb","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Stibich","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"},{"name":"Xenex Disinfection Services, San Antonio, TX 78216, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Maresso","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy F.","family":"Chemaly","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"de Kraker, M.E., Stewardson, A.J., and Harbarth, S. (2016). Will 10 Million People Die a Year due to Antimicrobial Resistance by 2050?. PLoS Med., 13.","DOI":"10.1371\/journal.pmed.1002184"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1017\/ice.2014.3","article-title":"Frequency, risk factors, and outcomes of vancomycin-resistant Enterococcus colonization and infection in patients with newly diagnosed acute leukemia: Different patterns in patients with acute myelogenous and acute lymphoblastic leukemia","volume":"36","author":"Ford","year":"2015","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/cncr.31045","article-title":"Clinical impact of colonization with multidrug-resistant organisms on outcome after allogeneic stem cell transplantation in patients with acute myeloid leukemia","volume":"124","author":"Scheich","year":"2018","journal-title":"Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"339ra71","DOI":"10.1126\/scitranslmed.aaf2311","article-title":"Increased GVHD-related mortality with broad-spectrum antibiotic use after allogeneic hematopoietic stem cell transplantation in human patients and mice","volume":"8","author":"Shono","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"ref_5","first-page":"5","article-title":"Enterococci and Their Interactions with the Intestinal Microbiome","volume":"5","author":"Dubin","year":"2014","journal-title":"Microbiol. Spectr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/nrmicro2761","article-title":"The rise of the Enterococcus: Beyond vancomycin resistance","volume":"10","author":"Arias","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_7","first-page":"79","article-title":"The role of the healthcare environment in the spread of multidrug-resistant organisms: Update on current best practices for containment","volume":"2","author":"Chemaly","year":"2014","journal-title":"Ther. Adv. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1093\/infdis\/jiv597","article-title":"Gastrointestinal Tract Colonization Dynamics by Different Enterococcus faecium Clades","volume":"213","author":"Montealegre","year":"2016","journal-title":"J. Infect. Dis"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"529","DOI":"10.4065\/81.4.529","article-title":"Vancomycin-resistant enterococci: Colonization, infection, detection, and treatment","volume":"81","author":"Zirakzadeh","year":"2006","journal-title":"Mayo Clin. Proc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/j.bbmt.2016.02.009","article-title":"Impact of Gut Colonization by Antibiotic-Resistant Bacteria on the Outcomes of Allogeneic Hematopoietic Stem Cell Transplantation: A Retrospective, Single-Center Study","volume":"22","author":"Bilinski","year":"2016","journal-title":"Biol. Blood Marrow Transplant"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4332","DOI":"10.1172\/JCI43918","article-title":"Vancomycin-resistant Enterococcus domination of intestinal microbiota is enabled by antibiotic treatment in mice and precedes bloodstream invasion in humans","volume":"120","author":"Ubeda","year":"2010","journal-title":"J. Clin. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1186\/s12967-015-0640-8","article-title":"Gut microbiota and allogeneic transplantation","volume":"13","author":"Wang","year":"2015","journal-title":"J. Transl. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"31","DOI":"10.4161\/bact.1.1.14942","article-title":"Phages in nature","volume":"1","author":"Clokie","year":"2011","journal-title":"Bacteriophage"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1038\/nrmicro3564","article-title":"A century of the phage: Past, present and future","volume":"13","author":"Salmond","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1016\/j.dld.2015.07.008","article-title":"The human gut microbiota and virome: Potential therapeutic implications","volume":"47","author":"Scarpellini","year":"2015","journal-title":"Dig. Liver Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.ijid.2008.01.006","article-title":"Is phage therapy acceptable in the immunocompromised host?","volume":"12","author":"Borysowski","year":"2008","journal-title":"Int. J. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2874","DOI":"10.1128\/AAC.49.7.2874-2878.2005","article-title":"Human volunteers receiving Escherichia coli phage T4 orally: A safety test of phage therapy","volume":"49","author":"Bruttin","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s00430-009-0135-4","article-title":"Prophylactic effect of bacteriophages on mice subjected to chemotherapy-induced immunosuppression and bone marrow transplant upon infection with Staphylococcus aureus","volume":"199","author":"Zimecki","year":"2010","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S155","DOI":"10.1016\/j.healun.2018.01.376","article-title":"(373)\u2014Bacteriophage Treatment in a Lung Transplant Recipient","volume":"37","author":"Aslam","year":"2018","journal-title":"J. Heart Lung Transpl."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1093\/emph\/eoy005","article-title":"Phage treatment of an aortic graft infected with Pseudomonas aeruginosa","volume":"2018","author":"Chan","year":"2018","journal-title":"Evol. Med. Public Health"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/s41591-019-0437-z","article-title":"Engineered bacteriophages for treatment of a patient with a disseminated drug-resistant Mycobacterium abscessus","volume":"25","author":"Dedrick","year":"2019","journal-title":"Nat. Med."},{"key":"ref_22","first-page":"167","article-title":"A Systematic and Critical Review of Bacteriophage Therapy against Multi-Drug Resistant ESKAPE Organisms in Humans","volume":"69","author":"Harb","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00954-17","DOI":"10.1128\/AAC.00954-17","article-title":"Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails to Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection","volume":"61","author":"Schooley","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.3389\/fmicb.2016.01383","article-title":"'Get in Early'; Biofilm and Wax Moth (Galleria mellonella) Models Reveal New Insights into the Therapeutic Potential of Clostridium difficile Bacteriophages","volume":"7","author":"Nale","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1080\/21505594.2015.1135289","article-title":"Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing","volume":"7","author":"Tsai","year":"2016","journal-title":"Virulence"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2828","DOI":"10.1128\/AEM.03852-12","article-title":"Characterization of a Novel Panton-Valentine leukocidin (PVL)-encoding staphylococcal phage and its naturally PVL-lacking variant","volume":"79","author":"Moineau","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"El Haddad, L., Ben Abdallah, N., Plante, P.-L., Dumaresq, J., Katsarava, R., Labrie, S., Corbeil, J., St-Gelais, D., and Moineau, S. (2014). Improving the Safety of Staphylococcus aureus Polyvalent Phages by Their Production on a Staphylococcus xylosus Strain. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0102600"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"8227","DOI":"10.1128\/AEM.02371-12","article-title":"Methicillin-resistant Staphylococcus aureus phage plaque size enhancement using sublethal concentrations of antibiotics","volume":"78","author":"Kaur","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s00705-006-0849-1","article-title":"5500 Phages examined in the electron microscope","volume":"152","author":"Ackermann","year":"2007","journal-title":"Arch. Virol."},{"key":"ref_30","unstructured":"Clokie, M., and Kropinski, A. (2009). Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions, Humana Press."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"10232","DOI":"10.1128\/JVI.01574-12","article-title":"Complete Genome of Staphylococcus aureus Phage SA11","volume":"86","author":"Kim","year":"2012","journal-title":"J. Virol."},{"key":"ref_32","first-page":"109","article-title":"Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes","volume":"Volume 3","author":"Clokie","year":"2018","journal-title":"Bacteriophages: Methods and Protocols"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Lo, C.C., and Chain, P.S. (2014). Rapid evaluation and quality control of next generation sequencing data with FaQCs. BMC Bioinform., 15.","DOI":"10.1186\/s12859-014-0366-2"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"8365","DOI":"10.1038\/srep08365","article-title":"RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes","volume":"5","author":"Brettin","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"D535","DOI":"10.1093\/nar\/gkw1017","article-title":"Improvements to PATRIC, the all-bacterial Bioinformatics Database and Analysis Resource Center","volume":"45","author":"Wattam","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/nar\/gkh152","article-title":"ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences","volume":"32","author":"Laslett","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"W5","DOI":"10.1093\/nar\/gkn201","article-title":"NCBI BLAST: A better web interface","volume":"36","author":"Johnson","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"D694","DOI":"10.1093\/nar\/gkv1239","article-title":"VFDB 2016: Hierarchical and refined dataset for big data analysis--10 years on","volume":"44","author":"Chen","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/bioinformatics\/btu631","article-title":"Curation, integration and visualization of bacterial virulence factors in PATRIC","volume":"31","author":"Mao","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"D443","DOI":"10.1093\/nar\/gkn656","article-title":"ARDB--Antibiotic Resistance Genes Database","volume":"37","author":"Liu","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3348","DOI":"10.1128\/AAC.00419-13","article-title":"The comprehensive antibiotic resistance database","volume":"57","author":"McArthur","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kaminski, J., Gibson, M.K., Franzosa, E.A., Segata, N., Dantas, G., and Huttenhower, C. (2015). High-Specificity Targeted Functional Profiling in Microbial Communities with ShortBRED. PLoS Comput. Biol., 11.","DOI":"10.1371\/journal.pcbi.1004557"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/ismej.2014.106","article-title":"Improved annotation of antibiotic resistance determinants reveals microbial resistomes cluster by ecology","volume":"9","author":"Gibson","year":"2015","journal-title":"ISME J"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1093\/nar\/gkw1027","article-title":"Enabling the democratization of the genomics revolution with a fully integrated web-based bioinformatics platform","volume":"45","author":"Li","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_45","unstructured":"Li, H. (2013). Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. arXiv."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"W16","DOI":"10.1093\/nar\/gkw387","article-title":"PHASTER: A better, faster version of the PHAST phage search tool","volume":"44","author":"Arndt","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1093\/bioinformatics\/bts014","article-title":"PHACTS, a computational approach to classifying the lifestyle of phages","volume":"28","author":"McNair","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1101\/gr.2289704","article-title":"Mauve: Multiple alignment of conserved genomic sequence with rearrangements","volume":"14","author":"Darling","year":"2004","journal-title":"Genome Res."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Alghoribi, M.F., Gibreel, T.M., Dodgson, A.R., Beatson, S.A., and Upton, M. (2014). Galleria mellonella infection model demonstrates high lethality of ST69 and ST127 uropathogenic E. coli. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0101547"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"419","DOI":"10.4161\/viru.24930","article-title":"Galleria mellonella larvae as an infection model for group A streptococcus","volume":"4","author":"Loh","year":"2013","journal-title":"Virulence"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Lossouarn, J., Briet, A., Moncaut, E., Furlan, S., Bouteau, A., Son, O., Leroy, M., DuBow, M.S., Lecointe, F., and Serror, P. (2019). Enterococcus faecalis Countermeasures Defeat a Virulent Picovirinae Bacteriophage. Viruses, 11.","DOI":"10.3390\/v11010048"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e9171","DOI":"10.7717\/peerj.9171","article-title":"Isolation, characterization, and comparative genomic analysis of a phage infecting high-level aminoglycoside-resistant (HLAR) Enterococcus faecalis","volume":"8","author":"Zhang","year":"2020","journal-title":"PeerJ"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1128\/JB.00259-12","article-title":"Comparative analysis of the first complete Enterococcus faecium genome","volume":"194","author":"Lam","year":"2012","journal-title":"J. Bacteriol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"326","DOI":"10.3389\/fmicb.2018.00326","article-title":"Defeating Antibiotic- and Phage-Resistant Enterococcus faecalis Using a Phage Cocktail in Vitro and in a Clot Model","volume":"9","author":"Khalifa","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1504\/IJCBDD.2014.061645","article-title":"Aeromonas phages encode tRNAs for their overused codons","volume":"7","author":"Prabhakaran","year":"2014","journal-title":"Int. J. Comput. Biol. Drug Des."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1111\/j.1574-6968.2007.00996.x","article-title":"Isolation and characterization of a novel Enterococcus faecalis bacteriophage phiEF24C as a therapeutic candidate","volume":"278","author":"Uchiyama","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2306","DOI":"10.1093\/cid\/ciab001","article-title":"Emergence and Transmission of Daptomycin and Vancomycin-Resistant Enterococci Between Patients and Hospital Rooms","volume":"73","author":"Hanson","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2336","DOI":"10.1093\/cid\/ciz666","article-title":"Outbreak of Vancomycin-resistant Enterococcus faecium in Interventional Radiology: Detection Through Whole-genome Sequencing-based Surveillance","volume":"70","author":"Sundermann","year":"2020","journal-title":"Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1093\/jac\/dkz097","article-title":"Emergence of optrA-mediated linezolid resistance in enterococci from France, 2006\u20132016","volume":"74","author":"Sassi","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3","DOI":"10.12688\/f1000research.11831.1","article-title":"Vancomycin-resistant enterococcus infection in the hematopoietic stem cell transplant recipient: An overview of epidemiology, management, and prevention","volume":"7","author":"Benamu","year":"2018","journal-title":"F1000resarch"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.ijantimicag.2015.04.005","article-title":"Assessing phage therapy against Pseudomonas aeruginosa using a Galleria mellonella infection model","volume":"46","author":"Beeton","year":"2015","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/B978-0-12-394805-2.00002-6","article-title":"Utility of Greater Wax Moth Larva (Galleria mellonella) for Evaluating the Toxicity and Efficacy of New Antimicrobial Agents","volume":"78","author":"Desbois","year":"2012","journal-title":"Adv. Appl. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1080\/21505594.2016.1203486","article-title":"Galleria mellonella as a model host for microbiological and toxin research","volume":"7","author":"Champion","year":"2016","journal-title":"Virulence"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1007\/s00705-014-2055-x","article-title":"Efficiency of bacteriophage therapy against Cronobacter sakazakii in Galleria mellonella (greater wax moth) larvae","volume":"159","author":"Abbasifar","year":"2014","journal-title":"Arch. Virol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Jeon, J., Park, J.-H., and Yong, D. (2019). Efficacy of bacteriophage treatment against carbapenem-resistant Acinetobacter baumannii in Galleria mellonella larvae and a mouse model of acute pneumonia. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1443-5"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"e02900","DOI":"10.1128\/AEM.02900-18","article-title":"Two Novel Bacteriophages Improve Survival in Galleria mellonella Infection and Mouse Acute Pneumonia Models Infected with Extensively Drug-Resistant Pseudomonas aeruginosa","volume":"85","author":"Jeon","year":"2019","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Manohar, P., Nachimuthu, R., and Lopes, B.S. (2018). The therapeutic potential of bacteriophages targeting gram-negative bacteria using Galleria mellonella infection model. BMC Microbiol., 18.","DOI":"10.1186\/s12866-018-1234-4"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1128\/IAI.70.1.204-210.2002","article-title":"Bacteriophage therapy rescues mice bacteremic from a clinical isolate of vancomycin-resistant Enterococcus faecium","volume":"70","author":"Biswas","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.virol.2016.02.006","article-title":"Characterization of Enterococcus faecium bacteriophage IME-EFm5 and its endolysin LysEFm5","volume":"492","author":"Gong","year":"2016","journal-title":"Virology"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1099\/vir.0.067553-0","article-title":"Characterization and complete genome sequence analysis of novel bacteriophage IME-EFm1 infecting Enterococcus faecium","volume":"95","author":"Wang","year":"2014","journal-title":"J. Gen. Virol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"3843","DOI":"10.1007\/s00705-017-3503-1","article-title":"Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium","volume":"162","author":"Xing","year":"2017","journal-title":"Arch. Virol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Paul, K., Merabishvili, M., Hazan, R., Christner, M., Herden, U., Gelman, D., Khalifa, L., Yerushalmy, O., Coppenhagen-Glazer, S., and Harbauer, T. (2021). Bacteriophage Rescue Therapy of a Vancomycin-Resistant Enterococcus faecium Infection in a One-Year-Old Child following a Third Liver Transplantation. 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