{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T05:52:11Z","timestamp":1791611531583,"version":"4.3.4"},"reference-count":67,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T00:00:00Z","timestamp":1661126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["20-65-46006"],"award-info":[{"award-number":["20-65-46006"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Traumatic brain injury (TBI) is a major public health problem. Here, we developed a novel model of non-invasive TBI induced by laser irradiation in the telencephalon of adult zebrafish (Danio rerio) and assessed their behavior and neuromorphology to validate the model and evaluate potential targets for neuroreparative treatment. Overall, TBI induced hypolocomotion and anxiety-like behavior in the novel tank test, strikingly recapitulating responses in mammalian TBI models, hence supporting the face validity of our model. NeuN-positive cell staining was markedly reduced one day, but not seven days, after TBI, suggesting increased neuronal damage immediately after the injury, and its fast recovery. The brain-derived neurotrophic factor (Bdnf) level in the brain dropped immediately after the trauma, but fully recovered seven days later. A marker of microglial activation, Iba1, was elevated in the TBI brain, albeit decreasing from Day 3. The levels of hypoxia-inducible factor 1-alpha (Hif1a) increased 30 min after the injury, and recovered by Day 7, further supporting the construct validity of the model. Collectively, these findings suggest that our model of laser-induced brain injury in zebrafish reproduces mild TBI and can be a useful tool for TBI research and preclinical neuroprotective drug screening.<\/jats:p>","DOI":"10.3390\/pharmaceutics14081751","type":"journal-article","created":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T21:30:34Z","timestamp":1661203834000},"page":"1751","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4265-8981","authenticated-orcid":false,"given":"Maria A.","family":"Tikhonova","sequence":"first","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Scientific Research Institute of Neurosciences and Medicine (SRINM), 630117 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai A.","family":"Maslov","sequence":"additional","affiliation":[{"name":"Khristianovich Institute of Theoretical and Applied Mechanics SB RAS, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9609-7297","authenticated-orcid":false,"given":"Alim A.","family":"Bashirzade","sequence":"additional","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Scientific Research Institute of Neurosciences and Medicine (SRINM), 630117 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenyi V.","family":"Nehoroshev","sequence":"additional","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladislav Y.","family":"Babchenko","sequence":"additional","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda D.","family":"Chizhova","sequence":"additional","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Scientific Research Institute of Neurosciences and Medicine (SRINM), 630117 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9303-1452","authenticated-orcid":false,"given":"Elena O.","family":"Tsibulskaya","sequence":"additional","affiliation":[{"name":"Khristianovich Institute of Theoretical and Applied Mechanics SB RAS, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna A.","family":"Akopyan","sequence":"additional","affiliation":[{"name":"Scientific Research Institute of Neurosciences and Medicine (SRINM), 630117 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeniya V.","family":"Markova","sequence":"additional","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Research Institute of Fundamental and Clinical Immunology (RIFCI), 630099 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ling","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwok-Tung","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7525-1950","authenticated-orcid":false,"given":"Allan V.","family":"Kalueff","sequence":"additional","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Scientific Research Institute of Neurosciences and Medicine (SRINM), 630117 Novosibirsk, Russia"},{"name":"Neurobiology Program, Sirius Science and Technology University, 354349 Sochi, Russia"},{"name":"Neuroscience Group, Ural Federal University, 620075 Yekaterinburg, Russia"},{"name":"Institute of Translational Biomedicine, St. Petersburg State University, 199034 St. Petersburg, Russia"},{"name":"Almazov National Medical Research Centre, 197341 St. Petersburg, Russia"},{"name":"Granov Russian Scientific Center of Radiology and Surgical Technologies, 1997758 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyubomir I.","family":"Aftanas","sequence":"additional","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Scientific Research Institute of Neurosciences and Medicine (SRINM), 630117 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2656-8687","authenticated-orcid":false,"given":"Tamara G.","family":"Amstislavskaya","sequence":"additional","affiliation":[{"name":"V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Scientific Research Institute of Neurosciences and Medicine (SRINM), 630117 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.3233\/NRE-2007-22502","article-title":"The impact of traumatic brain injuries: A global perspective","volume":"22","author":"Hyder","year":"2007","journal-title":"NeuroRehabilitation"},{"key":"ref_2","first-page":"134ra160","article-title":"Chronic traumatic encephalopathy in blast-exposed military veterans and a blast neurotrauma mouse model","volume":"4","author":"Goldstein","year":"2012","journal-title":"Sci. Transl. 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