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Project","award":["2019IIT23"],"award-info":[{"award-number":["2019IIT23"]}]},{"name":"Second Phase Project of Innovation Institute of High-Level University","award":["2020YFA0908800"],"award-info":[{"award-number":["2020YFA0908800"]}]},{"name":"Second Phase Project of Innovation Institute of High-Level University","award":["81871376"],"award-info":[{"award-number":["81871376"]}]},{"name":"Second Phase Project of Innovation Institute of High-Level University","award":["JCYJ20190812171820731"],"award-info":[{"award-number":["JCYJ20190812171820731"]}]},{"name":"Second Phase Project of Innovation Institute of High-Level University","award":["JCYJ20190807144209381"],"award-info":[{"award-number":["JCYJ20190807144209381"]}]},{"name":"Second Phase Project of Innovation Institute of High-Level University","award":["202102010383"],"award-info":[{"award-number":["202102010383"]}]},{"name":"Second Phase Project of Innovation Institute of High-Level University","award":["2019IIT23"],"award-info":[{"award-number":["2019IIT23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Osteonecrosis of the femoral head (ONFH) is a disease that is commonly seen in the clinic, but its detection rate remains limited, especially at the early stage. We developed an ultrasound molecular imaging (UMI) approach for early diagnosis of ONFH by detecting the expression of integrin \u03b1v\u03b23 during the pathological changes in steroid-induced osteonecrosis of the femoral head (SIONFH) in rat models. The integrin \u03b1v\u03b23-targeted PLGA or lipid microbubbles modified with iRGD peptides were fabricated and characterized. Their adhesion efficiency to mouse brain microvascular endothelial cells in vitro was examined, and the better LIPOiRGD was used for further in vivo molecular imaging of SIONFH rats at 1, 3 and 5 weeks; revealing significantly higher UMI signals could be observed in the 3-week and 5-week SIONFH rats but not in the 1-week SIONFH rats in comparison with the non-targeted microbubbles (32.75 \u00b1 0.95 vs. 0.17 \u00b1 0.09 for 5 weeks, p &lt; 0.05; 5.60 \u00b1 1.31 dB vs. 0.94 \u00b1 0.81 dB for 3 weeks, p &lt; 0.01; 1.13 \u00b1 0.13 dB vs. 0.73 \u00b1 0.31 dB for 1 week, p &gt; 0.05). These results were consistent with magnetic resonance imaging data and confirmed by immunofluorescence staining experiments. In conclusion, our study provides an alternative UMI approach to the early evaluation of ONFH.<\/jats:p>","DOI":"10.3390\/pharmaceutics14091898","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T01:40:13Z","timestamp":1662601213000},"page":"1898","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Imaging of Steroid-Induced Osteonecrosis of the Femoral Head through iRGD-Targeted Microbubbles"],"prefix":"10.3390","volume":"14","author":[{"given":"Ping","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Ultrasound, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510407, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510407, China"},{"name":"Department of Ultrasound, Suzhou Hospital of Anhui Medical University (Suzhou Municipal Hospital of Anhui Province), Suzhou 234000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3291-0482","authenticated-orcid":false,"given":"Jiaqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510407, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manlin","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Second People\u2019s Hospital of Shenzhen, The First Affiliated Hospital of Shenzhen University, Shenzhen 518061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Litao","family":"Sun","sequence":"additional","affiliation":[{"name":"Cancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People\u2019s Hospital (Affiliated People\u2019s Hospital), Hangzhou Medical College, Hangzhou 310014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Yan","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Circuit Design, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1007\/s00264-015-3079-x","article-title":"A prospective cohort study of the clinical presentation of non-traumatic osteonecrosis of the femoral head: Spine and knee symptoms as clinical presentation of hip osteonecrosis","volume":"40","author":"Hauzeur","year":"2016","journal-title":"Int. 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