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Due to the physical bactericidal mechanism of membrane disruption, antimicrobial peptides are considered as potential therapeutic candidates without inducing microbial resistance. Scorpion venom-derived peptide, Androctonus amoreuxi Antimicrobial Peptide 1 (AamAP1), has been proved to have broad-spectrum antimicrobial properties. However, AamAP1 can induce hemolysis and shows strong toxicity against mammalian cells. Herein, the antimicrobial activity and mechanism of a novel synthetic antimicrobial peptide, GK-19, derived from AamAP1 and its derivatives, was evaluated. Five bacteria and three fungi were used to evaluate the antimicrobial effects of GK-19 in vitro. Scalded mice models combined with skin and soft tissue infections (SSTIs) were used to evaluate its applicability. The results indicated that GK-19 could not only inhibit Gram-positive and Gram-negative bacterial growth, but also kill fungi by disrupting the microbial cell membrane. Meanwhile, GK-19 showed negligible toxicity to mammalian cells, low hemolytic activity and high stability in plasma. Furthermore, in scalded mice models combined with SSTIs induced by either Methicillin-Resistant Staphylococcus aureus (MRSA) or Candida albicans, GK-19 showed significant antimicrobial and healing effects. Overall, it was demonstrated that GK-19 might be a promising drug candidate in the battle against drug-resistant bacterial and fungal infections.<\/jats:p>","DOI":"10.3390\/pharmaceutics14091937","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T22:37:28Z","timestamp":1663108648000},"page":"1937","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3746-9489","authenticated-orcid":false,"given":"Chenghua","family":"Song","sequence":"first","affiliation":[{"name":"National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruichao","family":"Wen","sequence":"additional","affiliation":[{"name":"National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1107-7965","authenticated-orcid":false,"given":"Xiaoyan","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi","family":"Kou","sequence":"additional","affiliation":[{"name":"National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Second Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengkang","family":"Chang","sequence":"additional","affiliation":[{"name":"National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Lv","sequence":"additional","affiliation":[{"name":"National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"},{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongqian","family":"Wu","sequence":"additional","affiliation":[{"name":"National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an 710061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,13]]},"reference":[{"key":"ref_1","first-page":"63","article-title":"Rise in global antibiotic use","volume":"20","author":"Hofer","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e893","DOI":"10.1016\/S2542-5196(21)00280-1","article-title":"Global antibiotic consumption and usage in humans, 2000\u20132018: A spatial modelling study","volume":"5","author":"Browne","year":"2021","journal-title":"Lancet Planet. Health"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/s41579-020-0348-5","article-title":"Resisting antimicrobial resistance","volume":"18","author":"Nathan","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/S0140-6736(05)17907-0","article-title":"Outpatient antibiotic use in Europe and association with resistance: A cross-national database study","volume":"365","author":"Goossens","year":"2005","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/s41579-021-00649-x","article-title":"Antibiotic resistance in the environment","volume":"20","author":"Larsson","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1038\/s41564-020-0723-z","article-title":"A broad-spectrum antibiotic adjuvant reverses multidrug-resistant Gram-negative pathogens","volume":"5","author":"Song","year":"2020","journal-title":"Nat. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.tim.2018.12.010","article-title":"Alternatives to Conventional Antibiotics in the Era of Antimicrobial Resistance","volume":"27","author":"Ghosh","year":"2019","journal-title":"Trends Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1038\/s41579-018-0125-x","article-title":"The cost of antimicrobial resistance","volume":"17","author":"Hofer","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.tim.2017.11.005","article-title":"The Search for \u2018Evolution-Proof\u2019 Antibiotics","volume":"26","author":"Bell","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/s41579-019-0293-3","article-title":"Antibiotic development\u2014Economic, regulatory and societal challenges","volume":"18","author":"Balasegaram","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1038\/s41579-021-00585-w","article-title":"Antibiofilm activity of host defence peptides: Complexity provides opportunities","volume":"19","author":"Hancock","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"106434","DOI":"10.1016\/j.ijantimicag.2021.106434","article-title":"The cathelicidin-derived close-to-nature peptide D-11 sensitises Klebsiella pneumoniae to a range of antibiotics in vitro, ex vivo and in vivo","volume":"58","author":"Xu","year":"2021","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.ijantimicag.2019.07.014","article-title":"Eradication of meticillin-resistant Staphylococcus aureus from human skin by the novel LL-37-derived peptide P10 in four pharmaceutical ointments","volume":"54","author":"Nibbering","year":"2019","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.ijantimicag.2019.05.019","article-title":"Antimicrobial activity of two novel antimicrobial peptides AA139 and SET-M33 against clinically and genotypically diverse Klebsiella pneumoniae isolates with differing antibiotic resistance profiles","volume":"54","author":"Jongh","year":"2019","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/nrmicro1098","article-title":"Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria?","volume":"3","author":"Brogden","year":"2005","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/s41579-019-0167-8","article-title":"Tracking antibiotic mechanisms","volume":"17","author":"Pambos","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"26516","DOI":"10.1073\/pnas.1909585117","article-title":"The antimicrobial peptide ZY4 combats multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii infection","volume":"116","author":"Mwangi","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.peptides.2012.03.016","article-title":"Antimicrobial\/cytolytic peptides from the venom of the North African scorpion, Androctonus amoreuxi: Biochemical and functional characterization of natural peptides and a single site-substituted analog","volume":"35","author":"Almaaytah","year":"2012","journal-title":"Peptides"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"502","DOI":"10.3390\/ph7050502","article-title":"Enhanced Antimicrobial Activity of AamAP1-Lysine, a Novel Synthetic Peptide Analog Derived from the Scorpion Venom Peptide AamAP1","volume":"7","author":"Almaaytah","year":"2014","journal-title":"Pharmaceuticals"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Almaaytah, A., Farajallah, A., Abualhaijaa, A., and Al-Balas, Q. (2018). A3, a Scorpion Venom Derived Peptide Analogue with Potent Antimicrobial and Potential Antibiofilm Activity against Clinical Isolates of Multi-Drug Resistant Gram Positive Bacteria. Molecules, 23.","DOI":"10.20944\/preprints201805.0356.v1"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.2147\/IDR.S204626","article-title":"The evaluation of the synergistic antimicrobial and antibiofilm activity of AamAP1-Lysine with conventional antibiotics against representative resistant strains of both Gram-positive and Gram-negative bacteria","volume":"12","author":"Almaaytah","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5284","DOI":"10.1021\/acsnano.7b09109","article-title":"Self-Assembling Myristoylated Human \u03b1-Defensin 5 as a Next-Generation Nanobiotics Potentiates Therapeutic Efficacy in Bacterial Infection","volume":"12","author":"Lei","year":"2018","journal-title":"ACS Nano"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.ijantimicag.2014.12.015","article-title":"Efficacy and safety profile of the novel antimicrobial peptide PXL150 in a mouse model of infected burn wounds","volume":"45","author":"Noppa","year":"2015","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1128\/CMR.00029-06","article-title":"Epidemiology of Invasive Candidiasis: A Persistent Public Health Problem","volume":"20","author":"Pfaller","year":"2007","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1093\/cid\/ciab672","article-title":"Advancing Diagnostic Stewardship for Healthcare-Associated Infections, Antibiotic Resistance, and Sepsis","volume":"74","author":"Curren","year":"2022","journal-title":"Clin. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"baac011","DOI":"10.1093\/database\/baac011","article-title":"A review on antimicrobial peptides databases and the computational tools","volume":"2022","author":"Ramazi","year":"2022","journal-title":"Database"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"819563","DOI":"10.3389\/fonc.2022.819563","article-title":"Clinical Applications and Anticancer Effects of Antimicrobial Peptides: From Bench to Bedside","volume":"12","author":"Jafari","year":"2022","journal-title":"Front. Oncol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"616979","DOI":"10.3389\/fmicb.2021.616979","article-title":"Review: Lessons Learned from Clinical Trials Using Antimicrobial Peptides (AMPs)","volume":"12","author":"Dijksteel","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"001154","DOI":"10.1099\/mic.0.001154","article-title":"Bacterial pore-forming toxins","volume":"168","author":"Ulhuq","year":"2022","journal-title":"Microbiology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1002\/med.21879","article-title":"Antimicrobial peptides, conventional antibiotics, and their synergistic utility for the treatment of drug-resistant infections","volume":"42","author":"Zhu","year":"2022","journal-title":"Med. Res. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zhang, R., Xu, L., and Dong, C. (2022). Antimicrobial peptides: An overview of their structure, function and mechanism of action. Protein Pept. Lett., 29.","DOI":"10.2174\/0929866529666220613102145"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e202200239","DOI":"10.1002\/cbic.202200239","article-title":"Membrane Disruptive Action of Cationic Antibacterial Peptide B1CTcu3","volume":"23","author":"Ajayakumar","year":"2022","journal-title":"ChemBioChem"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Luo, Y., and Song, Y. (2021). Mechanism of Antimicrobial Peptides: Antimicrobial, Anti-Inflammatory and Antibiofilm Activities. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222111401"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e02340-16","DOI":"10.1128\/AAC.02340-16","article-title":"Intracellular Targeting Mechanisms by Antimicrobial Peptides","volume":"61","author":"Le","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1080\/10408410802143808","article-title":"Properties and Applications of Antimicrobial Peptides in Biodefense Against Biological Warfare Threat Agents","volume":"34","author":"Dawson","year":"2008","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1016\/j.peptides.2010.05.014","article-title":"Novel family of antimicrobial peptides from the skin of Rana shuchinae","volume":"31","author":"Zheng","year":"2010","journal-title":"Peptides"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1038\/ni.1944","article-title":"IL-37 is a fundamental inhibitor of innate immunity","volume":"11","author":"Nold","year":"2010","journal-title":"Nat. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1016\/j.peptides.2010.04.004","article-title":"Antimicrobial peptide of an anti-lipopolysaccharide factor modulates of the inflammatory response in RAW264.7 cells","volume":"31","author":"Lin","year":"2010","journal-title":"Peptides"},{"key":"ref_39","first-page":"1","article-title":"Cathelicidin LL-37: LPS-neutralizing, pleiotropic peptide","volume":"14","author":"Golec","year":"2007","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"24095","DOI":"10.1021\/acsami.1c02089","article-title":"Ultrafast Fabrication of Self-Healing and Injectable Carboxymethyl Chitosan Hydrogel Dressing for Wound Healing","volume":"13","author":"Cao","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"7078","DOI":"10.1021\/acsnano.1c00204","article-title":"Dual-Dynamic-Bond Cross-Linked Antibacterial Adhesive Hydrogel Sealants with On-Demand Removability for Post-Wound-Closure and Infected Wound Healing","volume":"15","author":"Liang","year":"2021","journal-title":"ACS Nano"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"12687","DOI":"10.1021\/acsnano.1c04206","article-title":"Functional Hydrogels as Wound Dressing to Enhance Wound Healing","volume":"15","author":"Liang","year":"2021","journal-title":"ACS Nano"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/s41579-020-00469-5","article-title":"Nanomaterials arising amid antibiotic resistance","volume":"19","author":"Gao","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s41579-020-0420-1","article-title":"Nanomaterial-based therapeutics for antibiotic-resistant bacterial infections","volume":"19","author":"Makabenta","year":"2020","journal-title":"Nat. Rev. Microbiol."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/14\/9\/1937\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:30:33Z","timestamp":1760142633000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/14\/9\/1937"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,13]]},"references-count":44,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["pharmaceutics14091937"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics14091937","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,13]]}}}