{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T12:14:37Z","timestamp":1779884077374,"version":"3.53.1"},"reference-count":44,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State of S\u00e3o Paulo Foundation\u2014FAPESP","award":["12\/50212-1"],"award-info":[{"award-number":["12\/50212-1"]}]},{"name":"State of S\u00e3o Paulo Foundation\u2014FAPESP","award":["17\/50333-7"],"award-info":[{"award-number":["17\/50333-7"]}]},{"name":"State of S\u00e3o Paulo Foundation\u2014FAPESP","award":["317118\/2021-8"],"award-info":[{"award-number":["317118\/2021-8"]}]},{"name":"Faculdade de Medicina Foundation","award":["12\/50212-1"],"award-info":[{"award-number":["12\/50212-1"]}]},{"name":"Faculdade de Medicina Foundation","award":["17\/50333-7"],"award-info":[{"award-number":["17\/50333-7"]}]},{"name":"Faculdade de Medicina Foundation","award":["317118\/2021-8"],"award-info":[{"award-number":["317118\/2021-8"]}]},{"name":"Brazilian National Council for Scientific and Technological Development (CNPq)","award":["12\/50212-1"],"award-info":[{"award-number":["12\/50212-1"]}]},{"name":"Brazilian National Council for Scientific and Technological Development (CNPq)","award":["17\/50333-7"],"award-info":[{"award-number":["17\/50333-7"]}]},{"name":"Brazilian National Council for Scientific and Technological Development (CNPq)","award":["317118\/2021-8"],"award-info":[{"award-number":["317118\/2021-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Aspergillosis is an invasive fungal disease associated with high mortality. Antifungal susceptibility testing (AFST) is receiving increasing consideration for managing patients, as well as for surveilling emerging drug resistance, despite having time-consuming and technically complex reference methodologies. The Sensititre YeastOne (SYO) and Etest methods are widely utilized for yeasts but have not been extensively evaluated for Aspergillus isolates. We obtained Posaconazole (POS), Voriconazole (VCZ), Itraconazole (ITC), Amphotericin B (AMB), Caspofungin (CAS), and Anidulafungin (AND) minimum inhibitory concentrations (MICs) for both the Etest (n = 330) and SYO (n = 339) methods for 106 sequenced clinical strains. For 84 A. fumigatus, we analyzed the performance of both commercial methods in comparison with the CLSI-AFST, using available cutoff values. An excellent correlation could be demonstrated for Etest-AMB and Etest-VCZ (p &lt; 0.01). SYO-MICs of AMB, VCZ, and POS resulted in excellent essential agreement (&gt;93%), and &gt;80% for AMB, VCZ, and ITC Etest-MICs. High categoric agreement was found for AMB, ITC, and CAS Etest-MICs (&gt;85%) and AMB SYO-MICs (&gt;90%). The considerable number of major\/very major errors found using Etest and SYO, possibly related to the proposed cutoffs and associated with the less time-consuming processes, support the need for the improvement of commercial methods for Aspergillus strains.<\/jats:p>","DOI":"10.3390\/pharmaceutics14102161","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T06:13:27Z","timestamp":1665468807000},"page":"2161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of the Sensititre YeastOne and Etest in Comparison with CLSI M38-A2 for Antifungal Susceptibility Testing of Three Azoles, Amphotericin B, Caspofungin, and Anidulafungin, against Aspergillusfumigatus and Other Species, Using New Clinical Breakpoints and Epidemiological Cutoff Values"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1335-8808","authenticated-orcid":false,"given":"Marcia S. C.","family":"Melhem","sequence":"first","affiliation":[{"name":"Mycology Unit, Parasitology and Mycology Department, Instituto Adolfo Lutz, Secretary of Health, Government of S\u00e3o Paulo State, S\u00e3o Paulo 01246-902, SP, Brazil"},{"name":"Graduate Program in Sciences, Coordination of Diseases Control, Secretary of Health, Government of S\u00e3o Paulo State, S\u00e3o Paulo 01246-902, SP, Brazil"},{"name":"Graduate Program in Infectious and Parasitic Diseases, Faculdade de Medicina, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2908-0801","authenticated-orcid":false,"given":"Vivian C.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica em Imunologia (LIM 48), Hospital das Cl\u00ednicas, Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia A.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica em Imunologia (LIM 48), Hospital das Cl\u00ednicas, Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidiane de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Mycology Unit, Parasitology and Mycology Department, Instituto Adolfo Lutz, Secretary of Health, Government of S\u00e3o Paulo State, S\u00e3o Paulo 01246-902, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas X.","family":"Bonfietti","sequence":"additional","affiliation":[{"name":"Mycology Unit, Parasitology and Mycology Department, Instituto Adolfo Lutz, Secretary of Health, Government of S\u00e3o Paulo State, S\u00e3o Paulo 01246-902, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria. W.","family":"Szeszs","sequence":"additional","affiliation":[{"name":"Mycology Unit, Parasitology and Mycology Department, Instituto Adolfo Lutz, Secretary of Health, Government of S\u00e3o Paulo State, S\u00e3o Paulo 01246-902, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello M. C.","family":"Magri","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica em Imunologia (LIM 48), Hospital das Cl\u00ednicas, Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"},{"name":"Division of Infectious Diseases, Hospital das Cl\u00ednicas da Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francine S.","family":"Dorneles","sequence":"additional","affiliation":[{"name":"Graduate Program in Infectious and Parasitic Diseases, Faculdade de Medicina, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Taguchi","sequence":"additional","affiliation":[{"name":"Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel V. S.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica em Imunologia (LIM 48), Hospital das Cl\u00ednicas, Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana L.","family":"Motta","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Division of Central Laboratory\u2014Laboratory of Medical Investigation\u2014LIM 03, Hospital das Cl\u00ednicas, Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjorie V.","family":"Batista","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Hospital das Cl\u00ednicas da Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiko","family":"Kamei","sequence":"additional","affiliation":[{"name":"Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3864-041X","authenticated-orcid":false,"given":"Maria A.","family":"Shikanai-Yasuda","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica em Imunologia (LIM 48), Hospital das Cl\u00ednicas, Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"},{"name":"Department of Infectious and Parasitic Diseases, Faculdade de Medicina, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1586\/eri.11.13","article-title":"Invasive Aspergillosis in Hematopoietic Stem Cell and Solid Organ Transplantation","volume":"9","author":"Salman","year":"2011","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1007\/s00134-007-0791-z","article-title":"Management of Invasive Pulmonary Aspergillosis in Non-Neutropenic Critically Ill Patients","volume":"33","author":"Trof","year":"2007","journal-title":"Intensive Care Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e149","DOI":"10.1016\/S1473-3099(20)30847-1","article-title":"Defining and Managing COVID-19-Associated Pulmonary Aspergillosis: The 2020 ECMM\/ISHAM Consensus Criteria for Research and Clinical Guidance","volume":"21","author":"Koehler","year":"2021","journal-title":"Lancet Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"ofab444","DOI":"10.1093\/ofid\/ofab444","article-title":"Antifungal Susceptibility Testing: A Primer for Clinicians","volume":"8","author":"Wiederhold","year":"2021","journal-title":"Open Forum Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1439-0507.2009.01813.x","article-title":"In Vitro Susceptibility Testing in Fungi: A Global Perspective on a Variety of Methods","volume":"53","author":"Perkhofer","year":"2010","journal-title":"Mycoses"},{"key":"ref_6","unstructured":"bioM\u00e9rieux, S.A. (2022, September 11). Summary of Etest Performance, Interpretative Criteria and Quality Control Ranges Table. Available online: https:\/\/www.biomerieux-usa.com\/sites\/subsidiary_us\/files\/supplementary_inserts_-_16273_-_b_-_en_-_eag_-_etest_application_guide-3.pdf."},{"key":"ref_7","unstructured":"bioM\u00e9rieux SA (2013). Etest Antifungal Susceptibility Testing Package Insert, bioM\u00e9rieux."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Sanguinetti, M., and Posteraro, B. (2018). Susceptibility Testing of Fungi to Antifungal Drugs. J. Fungi, 4.","DOI":"10.3390\/jof4030110"},{"key":"ref_9","unstructured":"Trek Diagnostic Systems. Sensititre Yeast One: Yeast One Susceptibility, Thermo Fisher Scientific. Available online: https:\/\/www.thermofisher.com\/br\/en\/home\/clinical\/clinical-microbiology\/antimicrobial-susceptibility-testing\/tests-accessories.html#plate-disc-families."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e00168-17","DOI":"10.1128\/AAC.00168-17","article-title":"Susceptibility Testing of Common and Uncommon Aspergillus Species against Posaconazole and Other Mold-Active Antifungal Azoles Using the Sensititre Method","volume":"61","author":"Mello","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Martho, K.F.C., de Melo, A.T., Takahashi, J.P.F., Guerra, J.M., da Silva Santos, D.C., Purisco, S.U., de Souza Carvalho Melhem, M., de Souza Carvalho Melhem, R., Phanord, C., and Sartorelli, P. (2016). Amino Acid Permeases and Virulence in Cryptococcus neoformans. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0163919"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e01597-19","DOI":"10.1128\/AAC.01597-19","article-title":"Comparison of the MICs Obtained by Gradient Concentration Strip and EUCAST Methods for Four Azole Drugs and Amphotericin B against Azole-Susceptible and Resistant Aspergillus Section Fumigati Clinical Isolates","volume":"64","author":"Verdurme","year":"2020","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_13","unstructured":"(2017). CLSI Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi M38A, Clinical and Laboratory Standards Institute. [3rd ed.]."},{"key":"ref_14","unstructured":"(2020). CLSI Epidemiological Cutoff Values for Antifungal Susceptibility Testing\u2014Supplement M59, Clinical and Laboratory Standards Institute."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.riam.2016.04.001","article-title":"The Role of Epidemiological Cutoff Values (ECVs\/ECOFFs) in Antifungal Susceptibility Testing and Interpretation for Uncommon Yeasts and Moulds","volume":"33","author":"Turnidge","year":"2016","journal-title":"Rev. Iberoam. Micol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1128\/aem.61.4.1323-1330.1995","article-title":"Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes","volume":"61","author":"Glass","year":"1995","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1128\/EC.00162-06","article-title":"Molecular Studies Reveal Frequent Misidentification of Aspergillus fumigatus by Morphotyping","volume":"5","author":"Balajee","year":"2006","journal-title":"Eukaryot. Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1099\/ijs.0.63980-0","article-title":"Novel Neosartorya Species Isolated from Soil in Korea","volume":"56","author":"Hong","year":"2006","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_19","first-page":"315","article-title":"Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics","volume":"Volume 31","author":"White","year":"1990","journal-title":"PCR Protocols: A Guide to Methods and Applications"},{"key":"ref_20","unstructured":"(2020). CLSI Performance Standards for Antifungal Susceptibility Testing of Filamentous Fungi\u2014M61, Clinical and Laboratory Standards Institute. [2nd ed.]."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Espinel-Ingroff, A. (2022). Commercial Methods for Antifungal Susceptibility Testing of Yeasts: Strengths and Limitations as Predictors of Resistance. J. Fungi, 8.","DOI":"10.3390\/jof8030309"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Dannaoui, E., and Espinel-Ingroff, A. (2019). Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review. J. Fungi, 5.","DOI":"10.3390\/jof5040108"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"720609","DOI":"10.3389\/fcimb.2021.720609","article-title":"Can We Improve Antifungal Susceptibility Testing?","volume":"11","author":"Durand","year":"2021","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1128\/JCM.00925-15","article-title":"Comparing Etest and Broth Microdilution for Antifungal Susceptibility Testing of the Most-Relevant Pathogenic Molds","volume":"53","author":"Lamoth","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3944","DOI":"10.1128\/AAC.00428-11","article-title":"In Vitro Echinocandin Susceptibility of Aspergillus Isolates from Patients Enrolled in the Transplant-Associated Infection Surveillance Network","volume":"55","author":"Pfaller","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e01916-17","DOI":"10.1128\/AAC.01916-17","article-title":"Posaconazole MIC Distributions for Aspergillus fumigatus Species Complex by Four Methods: Impact of Cyp51A Mutations on Estimation of Epidemiological Cutoff Values","volume":"62","author":"Turnidge","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e01651-18","DOI":"10.1128\/AAC.01651-18","article-title":"Method-Dependent Epidemiological Cutoff Values for Detection of Triazole Resistance in Candida and Aspergillus Species for the Sensititre YeastOne Colorimetric Broth and Etest Agar Diffusion Methods","volume":"63","author":"Turnidge","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e01093-21","DOI":"10.1128\/AAC.01093-21","article-title":"Etest ECVs\/ECOFFs for Detection of Resistance in Prevalent and Three Nonprevalent Candida spp. to Triazoles and Amphotericin B and Aspergillus spp. to Caspofungin: Further Assessment of Modal Variability","volume":"65","author":"Sasso","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s11046-014-9730-z","article-title":"Evaluation of Etest Performed in Mueller-Hinton Agar Supplemented with Glucose for Antifungal Susceptibility Testing of Clinical Isolates of Filamentous Fungi","volume":"177","author":"Pinto","year":"2014","journal-title":"Mycopathologia"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.1128\/JCM.40.8.2876-2885.2002","article-title":"Comparison of the Etest and the Sensititre Colorimetric Methods with the NCCLS Proposed Standard for Antifungal Susceptibility Testing of Aspergillus Species","volume":"40","author":"Meletiadis","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1093\/jac\/dkg384","article-title":"Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel and Etest with the NCCLS M38-A Method to Determine the Activity of Amphotericin B and Itraconazole against Clinical Isolates of Aspergillus spp","volume":"52","author":"Valverde","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1128\/JCM.40.6.2101-2107.2002","article-title":"E-Test Method for Testing Susceptibilities of Aspergillus Spp. to the New Triazoles Voriconazole and Posaconazole and to Established Antifungal Agents: Comparison with NCCLS Broth Microdilution Method","volume":"40","author":"Rezusta","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5270","DOI":"10.1128\/JCM.41.11.5270-5272.2003","article-title":"Comparison of Etest with Modified Broth Microdilution Method for Testing Susceptibility of Aspergillus spp. to Voriconazole","volume":"41","author":"Serrano","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1128\/JCM.39.4.1360-1367.2001","article-title":"Comparison of the E-Test with the NCCLS M38-P Method for Antifungal Susceptibility Testing of Common and Emerging Pathogenic Filamentous Fungi","volume":"39","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s12281-020-00379-2","article-title":"Azole-Resistant Aspergillus and Echinocandin-Resistant Candida: What Are the Treatment Options?","volume":"14","author":"Novak","year":"2020","journal-title":"Curr. Fungal Infect. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s11046-017-0208-7","article-title":"Comparison Between Etest and Broth Microdilution Methods for Testing Itraconazole-Resistant Aspergillus fumigatus Susceptibility to Antifungal Combinations","volume":"183","author":"Denardi","year":"2018","journal-title":"Mycopathologia"},{"key":"ref_37","unstructured":"Berkow, E., and Lockhart, S.R. (2022, September 13). Ostrosky-Zeichner Antifungal Susceptibility Testing: Current Approaches. Available online: https:\/\/journals.asm.org\/doi\/epdf\/10.1128\/CMR.00069-19."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e00780-18","DOI":"10.1128\/JCM.00780-18","article-title":"Comparison of the Sensititre YeastOne and CLSI M38-A2 Microdilution Methods in Determining the Activity of Amphotericin B, Itraconazole, Voriconazole, and Posaconazole against Aspergillus Species","volume":"56","author":"Wang","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1128\/JCM.00287-07","article-title":"Clinical Evaluation of the Sensititre YeastOne Plate for Testing Susceptibility of Filamentous Fungi to Posaconazole","volume":"45","author":"Patel","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3616","DOI":"10.1128\/JCM.01187-06","article-title":"Comparison of Three Commercial Assays and a Modified Disk Diffusion Assay with Two Broth Microdilution Reference Assays for Testing Zygomycetes, Aspergillus spp., Candida spp., and Cryptococcus neoformans with Posaconazole and Amphotericin B","volume":"44","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.diagmicrobio.2006.03.004","article-title":"Comparison of Sensititre YeastOne\u00ae with the NCCLS M38-A Microdilution Method to Determine the Activity of Amphotericin B, Voriconazole, and Itraconazole against Clinical Isolates of Aspergillus fumigatus","volume":"56","author":"Guinea","year":"2006","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1016\/j.cmi.2019.04.027","article-title":"Multicentre Study to Determine the Etest Epidemiological Cut-off Values of Antifungal Drugs in Candida spp. and Aspergillus fumigatus Species Complex","volume":"25","author":"Gangneux","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1128\/JCM.00044-17","article-title":"Comparative Evaluation of Sensititre YeastOne and CLSI M38-A2 Reference Method for Antifungal Susceptibility Testing of Aspergillus spp. against Echinocandins","volume":"55","author":"Siopi","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"947","DOI":"10.2217\/fmb.14.55","article-title":"The future of fungal susceptibility testing","volume":"9","author":"Posteraro","year":"2014","journal-title":"Future Microbiol."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/14\/10\/2161\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:49:45Z","timestamp":1760143785000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/14\/10\/2161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,11]]},"references-count":44,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["pharmaceutics14102161"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics14102161","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,11]]}}}