{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:13:46Z","timestamp":1790957626338,"version":"4.1.0"},"reference-count":93,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T00:00:00Z","timestamp":1668816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Science, Innovation, and Universities and the Spanish State Research Agency","award":["RTI2018-094579-B-I00"],"award-info":[{"award-number":["RTI2018-094579-B-I00"]}]},{"name":"Spanish Ministry of Science, Innovation, and Universities and the Spanish State Research Agency","award":["2017-SGR-908"],"award-info":[{"award-number":["2017-SGR-908"]}]},{"name":"Spanish Ministry of Science, Innovation, and Universities and the Spanish State Research Agency","award":["USP-05-2019"],"award-info":[{"award-number":["USP-05-2019"]}]},{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya, Spain","doi-asserted-by":"publisher","award":["RTI2018-094579-B-I00"],"award-info":[{"award-number":["RTI2018-094579-B-I00"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya, Spain","doi-asserted-by":"publisher","award":["2017-SGR-908"],"award-info":[{"award-number":["2017-SGR-908"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya, Spain","doi-asserted-by":"publisher","award":["USP-05-2019"],"award-info":[{"award-number":["USP-05-2019"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Uni\u00f3n Iberoamericana de Universidades","award":["RTI2018-094579-B-I00"],"award-info":[{"award-number":["RTI2018-094579-B-I00"]}]},{"name":"Uni\u00f3n Iberoamericana de Universidades","award":["2017-SGR-908"],"award-info":[{"award-number":["2017-SGR-908"]}]},{"name":"Uni\u00f3n Iberoamericana de Universidades","award":["USP-05-2019"],"award-info":[{"award-number":["USP-05-2019"]}]},{"name":"Schlumberger Foundation Faculty","award":["RTI2018-094579-B-I00"],"award-info":[{"award-number":["RTI2018-094579-B-I00"]}]},{"name":"Schlumberger Foundation Faculty","award":["2017-SGR-908"],"award-info":[{"award-number":["2017-SGR-908"]}]},{"name":"Schlumberger Foundation Faculty","award":["USP-05-2019"],"award-info":[{"award-number":["USP-05-2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>The methyl erythritol phosphate (MEP) pathway of isoprenoid biosynthesis is essential for malaria parasites and also for several human pathogenic bacteria, thus representing an interesting target for future antimalarials and antibiotics and for diagnostic strategies. We have developed a DNA aptamer (D10) against Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), the second enzyme of this metabolic route. D10 binds in vitro to recombinant DXR from P. falciparum and Escherichia coli, showing at 10 \u00b5M a ca. 50% inhibition of the bacterial enzyme. In silico docking analysis indicates that D10 associates with DXR in solvent-exposed regions outside the active center pocket. According to fluorescence confocal microscopy data, this aptamer specifically targets in P. falciparum in vitro cultures the apicoplast organelle where the MEP pathway is localized and is, therefore, a highly specific marker of red blood cells parasitized by Plasmodium vs. na\u00efve erythrocytes. D10 is also selective for the detection of MEP+ bacteria (e.g., E. coli and Pseudomonas aeruginosa) vs. those lacking DXR (e.g., Enterococcus faecalis). Based on these results, we discuss the potential of DNA aptamers in the development of ligands that can outcompete the performance of the well-established antibody technology for future therapeutic and diagnostic approaches.<\/jats:p>","DOI":"10.3390\/pharmaceutics14112515","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T03:09:30Z","timestamp":1669000170000},"page":"2515","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from Plasmodium falciparum"],"prefix":"10.3390","volume":"14","author":[{"given":"Carlota","family":"Roca","sequence":"first","affiliation":[{"name":"Barcelona Institute for Global Health (ISGlobal), Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"},{"name":"Nanomalaria Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, 08028 Barcelona, Spain"},{"name":"Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Mart\u00ed i Franqu\u00e8s 1, 08028 Barcelona, Spain"},{"name":"Department of Biochemistry and Molecular Biomedicine, University of Barcelona, Avda. Diagonal 643, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4074-5624","authenticated-orcid":false,"given":"Yunuen","family":"Avalos-Padilla","sequence":"additional","affiliation":[{"name":"Barcelona Institute for Global Health (ISGlobal), Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"},{"name":"Nanomalaria Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, 08028 Barcelona, Spain"},{"name":"Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Mart\u00ed i Franqu\u00e8s 1, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8016-1565","authenticated-orcid":false,"given":"Beatriz","family":"Prieto-Sim\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Electronic Engineering, Universitat Rovira i Virgili, 43007 Tarragona, Spain"},{"name":"ICREA, Pg. Llu\u00eds Companys 23, 08010 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6133-0869","authenticated-orcid":false,"given":"Valent\u00edn","family":"Iglesias","sequence":"additional","affiliation":[{"name":"Barcelona Institute for Global Health (ISGlobal), Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"},{"name":"Nanomalaria Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, 08028 Barcelona, Spain"},{"name":"Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Mart\u00ed i Franqu\u00e8s 1, 08028 Barcelona, Spain"},{"name":"Institut de Biotecnologia i Biomedicina and Departament de Bioqu\u00edmica i Biologia Molecular, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Barcelona Institute for Global Health (ISGlobal), Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-1428","authenticated-orcid":false,"given":"Santiago","family":"Imperial","sequence":"additional","affiliation":[{"name":"Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Mart\u00ed i Franqu\u00e8s 1, 08028 Barcelona, Spain"},{"name":"Department of Biochemistry and Molecular Biomedicine, University of Barcelona, Avda. Diagonal 643, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4622-9631","authenticated-orcid":false,"given":"Xavier","family":"Fern\u00e0ndez-Busquets","sequence":"additional","affiliation":[{"name":"Barcelona Institute for Global Health (ISGlobal), Hospital Cl\u00ednic, Universitat de Barcelona, Rossell\u00f3 149-153, 08036 Barcelona, Spain"},{"name":"Nanomalaria Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, 08028 Barcelona, Spain"},{"name":"Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Mart\u00ed i Franqu\u00e8s 1, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,19]]},"reference":[{"key":"ref_1","unstructured":"(2022, July 13). World Health Organization. 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