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The single crystal X-ray analysis of the 2a complex revealed that a mononuclear and a dinuclear complex co-crystallize in the solid state. The electronic structures of the complexes are optimized by Density Functional Theory (DFT) calculations. The monomeric nature of 2a and 2b species is maintained in solution. Antioxidant activities of the ligands (1a, b) and Cu(II) complexes (2a, b) were determined by in vitro assays such as 1,1-diphenyl-2-picrylhydrazyl free radicals (DPPH.) and 2,2\u2032-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radicals (ABTS+). Our results demonstrated that 2a showed better antioxidant activity. MTT assays were performed to assess the toxicity of ligands and Cu(II) complexes in V79 cells. The antiproliferative activity of compounds was tested against two human tumor cell lines: MCF-7 (breast adenocarcinoma) and SW620 (colorectal carcinoma) and on MRC-5 (normal lung fibroblast). All compounds showed high cytotoxicity in the all-cell lines but showed no selectivity for tumor cell lines. Antiproliferative activity by clonogenic assay 2b showed a more significant inhibitory effect on the MCF-7 cell lines than on MRC-5. DNA damage for the 2b compound at 10 \u00b5M concentration was about three times higher in MCF-7 cells than in MRC-5 cells.<\/jats:p>","DOI":"10.3390\/pharmaceutics15020376","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T03:26:41Z","timestamp":1674444401000},"page":"376","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Antioxidant and Anticancer Potential of the New Cu(II) Complexes Bearing Imine-Phenolate Ligands with Pendant Amine N-Donor Groups"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4092-9896","authenticated-orcid":false,"given":"Adriana Castro","family":"Pinheiro","sequence":"first","affiliation":[{"name":"Laboratory of Genetic Toxicology, Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre 90050-170, RS, Brazil"},{"name":"Group of Catalysis of Theoretical 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Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9621-688X","authenticated-orcid":false,"given":"Paula Pellenz","family":"Tomasini","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Toxicology, Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre 90050-170, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6128-5025","authenticated-orcid":false,"given":"Cristiano","family":"Trindade","sequence":"additional","affiliation":[{"name":"Laboratory of Genetic Toxicology, Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre 90050-170, RS, Brazil"},{"name":"Centro de Investigaciones en Ciencias de la Vida, Universidad Sim\u00f3n Bol\u00edvar, Barranquilla 080002, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Crist\u00f3v\u00e3o","family":"Martins","sequence":"additional","affiliation":[{"name":"Laboratory in Biochemical Pharmacology, Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas (UFPel), Pelotas 96160-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ethel Antunes","family":"Wilhelm","sequence":"additional","affiliation":[{"name":"Laboratory in Biochemical Pharmacology, Center of Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas (UFPel), Pelotas 96160-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5376-0527","authenticated-orcid":false,"given":"Robson da Silva","family":"Oliboni","sequence":"additional","affiliation":[{"name":"Group of Catalysis of Theoretical Studies, Center of Chemical, Pharmaceutical and Food Science Center, Federal University of Pelotas (UFPel), Pelotas 96160-000, RS, 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