{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:04:07Z","timestamp":1778637847354,"version":"3.51.4"},"reference-count":57,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016971","name":"M.V. Lomonosov Moscow State University","doi-asserted-by":"publisher","award":["121041500039-8"],"award-info":[{"award-number":["121041500039-8"]}],"id":[{"id":"10.13039\/501100016971","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016971","name":"M.V. Lomonosov Moscow State University","doi-asserted-by":"publisher","award":["AAAA-A21-121011190061-1"],"award-info":[{"award-number":["AAAA-A21-121011190061-1"]}],"id":[{"id":"10.13039\/501100016971","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Helmholtz National Medical Research Center of Eye Diseases","award":["121041500039-8"],"award-info":[{"award-number":["121041500039-8"]}]},{"name":"Helmholtz National Medical Research Center of Eye Diseases","award":["AAAA-A21-121011190061-1"],"award-info":[{"award-number":["AAAA-A21-121011190061-1"]}]},{"name":"Ministry of Science and Higher Education","award":["121041500039-8"],"award-info":[{"award-number":["121041500039-8"]}]},{"name":"Ministry of Science and Higher Education","award":["AAAA-A21-121011190061-1"],"award-info":[{"award-number":["AAAA-A21-121011190061-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Improvement of the efficiency of drug penetration into the eye tissues is still an actual problem in ophthalmology. One of the most promising solutions is drug encapsulation in carriers capable of overcoming the cornea\/sclera tissue barrier. Formulations on the base of antioxidant enzyme, superoxide dismutase 1 (SOD1), and an inhibitor of angiotensin-converting enzyme, enalaprilat, were prepared by simultaneous inclusion of both drugs into calcium phosphate (CaP) particles in situ with subsequent covering of the particles with 5 kDa chitosan. The formulations obtained were characterized by dynamic light scattering and scanning electron microscopy. Hybrid CaP-chitosan particles co-loaded with SOD1 and enalaprilat had a mean hydrodynamic diameter of 120\u2013160 nm and \u03b6-potential +20 \u00b1 1 mV. The percentage of the inclusion of SOD1 and enalaprilat in hybrid particles was 30% and 56%, respectively. The ability of SOD1 and enalaprilat to reduce intraocular pressure (IOP) was examined in vivo in normotensive Chinchilla rabbits. It was shown that topical instillations of SOD1\/enalaprilat co-loaded hybrid particles were much more effective in decreasing IOP compared to free enzyme or free enalaprilat and even to the same particles that contained a single drug. Thus, the proposed formulations demonstrate potential as prospective therapeutic agents for the treatment of glaucoma.<\/jats:p>","DOI":"10.3390\/pharmaceutics15020550","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T02:28:17Z","timestamp":1675736897000},"page":"550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Chitosan-Covered Calcium Phosphate Particles Co-Loaded with Superoxide Dismutase 1 and ACE Inhibitor: Development, Characterization and Effect on Intraocular Pressure"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2419-2823","authenticated-orcid":false,"given":"Ekaterina","family":"Popova","sequence":"first","affiliation":[{"name":"Chemistry Faculty, M.V. 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Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Kudryashova","sequence":"additional","affiliation":[{"name":"Chemistry Faculty, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2358-0784","authenticated-orcid":false,"given":"Yuri","family":"Grigoriev","sequence":"additional","affiliation":[{"name":"Shubnikov Institute of Crystallography, Federal Scientific Research Center Crystallography and Photonics, Russian Academy of Sciences, 119333 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Chesnokova","sequence":"additional","affiliation":[{"name":"Helmholtz National Medical Research Center of Eye Diseases, 105062 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9665-0523","authenticated-orcid":false,"given":"Olga","family":"Kost","sequence":"additional","affiliation":[{"name":"Chemistry Faculty, M.V. 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