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The progression of chronic myeloid leukemia (CML) is frequently associated with an increased vascular density in bone marrow. From a molecular point of view, the small GTP-binding protein Rab11a, involved in the endosomal slow recycling pathway, has been shown to play a crucial role for the neoangiogenic process at the bone marrow of CML patients, by controlling the secretion of exosomes by CML cells, and by regulating the recycling of vascular endothelial factor receptors. The angiogenic potential of exosomes secreted by the CML cell line K562 has been previously observed using the chorioallantoic membrane (CAM) model. Herein, gold nanoparticles (AuNPs) were functionalized with an anti-RAB11A oligonucleotide (AuNP@RAB11A) to downregulate RAB11A mRNA in K562 cell line which showed a 40% silencing of the mRNA after 6 h and 14% silencing of the protein after 12 h. Then, using the in vivo CAM model, these exosomes secreted by AuNP@RAB11A incubated K562 did not present the angiogenic potential of those secreted from untreated K562 cells. These results demonstrate the relevance of Rab11 for the neoangiogenesis mediated by tumor exosomes, whose deleterious effect may be counteracted via targeted silencing of these crucial genes; thus, decreasing the number of pro-tumoral exosomes at the tumor microenvironment.<\/jats:p>","DOI":"10.3390\/pharmaceutics15030742","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T03:56:58Z","timestamp":1677124618000},"page":"742","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Exploring RAB11A Pathway to Hinder Chronic Myeloid Leukemia-Induced Angiogenesis In Vivo"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8676-6562","authenticated-orcid":false,"given":"Catarina","family":"Roma-Rodrigues","sequence":"first","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2054-4438","authenticated-orcid":false,"given":"Alexandra R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5255-7095","authenticated-orcid":false,"given":"Pedro V.","family":"Baptista","sequence":"additional","affiliation":[{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/315550a0","article-title":"Fused transcript of abl and bcr genes in chronic myelogenous leukaemia","volume":"315","author":"Shtivelman","year":"1985","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101194","DOI":"10.1016\/j.beha.2020.101194","article-title":"CML-Not only BCR-ABL1 matters","volume":"33","author":"Rinke","year":"2020","journal-title":"Best Pract. Res. Clin. Haematol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s11899-022-00679-z","article-title":"Therapy Resistance and Disease Progression in CML: Mechanistic Links and Therapeutic Strategies","volume":"17","author":"Ng","year":"2022","journal-title":"Curr. Hematol. Malign Rep."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Abdulmawjood, B., Costa, B., Roma-Rodrigues, C., Baptista, P.V., and Fernandes, A.R. (2021). Genetic Biomarkers in Chronic Myeloid Leukemia: What Have We Learned So Far?. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222212516"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1038\/s41375-020-0776-2","article-title":"European LeukemiaNet 2020 recommendations for treating chronic myeloid leukemia","volume":"34","author":"Hochhaus","year":"2020","journal-title":"Leukemia"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/sj.leu.2402769","article-title":"Clinicopathologic correlations of bone marrow angiogenesis in chronic myeloid leukemia: A morphometric study","volume":"17","author":"Korkolopoulou","year":"2003","journal-title":"Leukemia"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"241","DOI":"10.5114\/pjp.2017.71532","article-title":"The significance of angiogenesis for predicting optimal therapeutic response in chronic myeloid leukaemia patients","volume":"68","author":"Cojbasic","year":"2017","journal-title":"Pol. J. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1182\/asheducation-2011.1.1","article-title":"Angiogenesis: A Target in Solid Tumors, Also in Leukemia?","volume":"2011","author":"Schmidt","year":"2011","journal-title":"Hematol. Am. Soc. Hematol. Educ. Program Book"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"111934","DOI":"10.1016\/j.yexcr.2020.111934","article-title":"Apatinib exhibits cytotoxicity toward leukemia cells by targeting VEGFR2-mediated prosurvival signaling and angiogenesis","volume":"390","author":"Deng","year":"2020","journal-title":"Exp. Cell Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5008","DOI":"10.1039\/C8CS01021H","article-title":"Nanotechnology for angiogenesis: Opportunities and challenges","volume":"49","author":"Kargozar","year":"2020","journal-title":"Chem. Soc. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s12943-019-0991-5","article-title":"Exosomes: Composition, biogenesis, and mechanisms in cancer metastasis and drug resistance","volume":"18","author":"Mashouri","year":"2019","journal-title":"Mol. Cancer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1111\/jcmm.12873","article-title":"Chronic myelogenous leukaemia exosomes modulate bone marrow microenvironment through activation of epidermal growth factor receptor","volume":"20","author":"Corrado","year":"2016","journal-title":"J. Cell. Mol. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s12964-015-0086-x","article-title":"Chronic myeloid leukemia-derived exosomes promote tumor growth through an autocrine mechanism","volume":"13","author":"Raimondo","year":"2015","journal-title":"Cell Commun. Signal."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1186\/1476-4598-13-169","article-title":"Exosomal shuttling of miR-126 in endothelial cells modulates adhesive and migratory abilities of chronic myelogenous leukemia cells","volume":"13","author":"Taverna","year":"2014","journal-title":"Mol. Cancer"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s10456-011-9241-1","article-title":"Exosomes released by K562 chronic myeloid leukemia cells promote angiogenesis in a Src-dependent fashion","volume":"15","author":"Mineo","year":"2012","journal-title":"Angiogenesis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1002\/ijc.26217","article-title":"Role of exosomes released by chronic myelogenous leukemia cells in angiogenesis","volume":"130","author":"Taverna","year":"2012","journal-title":"Int. J. Cancer"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6843","DOI":"10.2147\/IJN.S215711","article-title":"Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles","volume":"14","author":"Fernandes","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.tcb.2014.02.004","article-title":"Orchestration of cell surface proteins by Rab11","volume":"24","author":"Welz","year":"2014","journal-title":"Trends Cell Biol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zhao, L., Yu, Q., Gao, C., Xiang, J., Zheng, B., Feng, Y., Li, R., Zhang, W., Hong, X., and Zhan, Y.Y. (2022). Studies of the Efficacy of Low-Dose Apatinib Monotherapy as Third-Line Treatment in Patients with Metastatic Colorectal Cancer and Apatinib\u2019s Novel Anticancer Effect by Inhibiting Tumor-Derived Exosome Secretion. Cancers, 14.","DOI":"10.3390\/cancers14102492"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1182\/blood-2011-01-328773","article-title":"Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output","volume":"118","author":"Andersson","year":"2011","journal-title":"Blood"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Jung, J.-J., Tiwari, A., Inamdar, S.M., Thomas, C.P., Goel, A., and Choudhury, A. (2012). Secretion of Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1\/sFlt1) Requires Arf1, Arf6, and Rab11 GTPases. PLoS ONE, 7.","DOI":"10.1371\/annotation\/19ed28f9-9a01-4af9-857d-3ec4aaeceea1"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1002\/mco2.9","article-title":"Intermedin promotes vessel fusion by inducing VE-cadherin accumulation at potential fusion sites and to achieve a dynamic balance between VE-cadherin-complex dissociation\/reconstitution","volume":"1","author":"Kong","year":"2020","journal-title":"MedComm"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e03720","DOI":"10.7554\/eLife.03720","article-title":"Neuropilin-1 functions as a VEGFR2 co-receptor to guide developmental angiogenesis independent of ligand binding","volume":"3","author":"Gelfand","year":"2014","journal-title":"Elife"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4939-2569-8_1","article-title":"Rab family of GTPases","volume":"1298","author":"Li","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e21679","DOI":"10.1096\/fj.202100286R","article-title":"Endothelial neuropilin-2 influences angiogenesis by regulating actin pattern development and \u03b15-integrin-p-FAK complex recruitment to assembling adhesion sites","volume":"35","author":"Benwell","year":"2021","journal-title":"FASEB J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1080\/21541248.2016.1264352","article-title":"New insights into the function of Rab GTPases in the context of exosomal secretion","volume":"9","author":"Blanc","year":"2018","journal-title":"Small GTPases"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1242\/jcs.115.12.2505","article-title":"The exosome pathway in K562 cells is regulated by Rab11","volume":"115","author":"Savina","year":"2002","journal-title":"J. Cell Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1600-0854.2004.00257.x","article-title":"Rab11 promotes docking and fusion of multivesicular bodies in a calcium-dependent manner","volume":"6","author":"Savina","year":"2005","journal-title":"Traffic"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e1817","DOI":"10.1002\/wnan.1817","article-title":"Recent developments in the use of gold and silver nanoparticles in biomedicine","volume":"14","author":"Pasparakis","year":"2022","journal-title":"Wiley Interdiscip. Rev. Nanomed. Nanobiotechnology"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Roma-Rodrigues, C., Rivas-Garc\u00eda, L., Baptista, P.V., and Fernandes, A.R. (2020). Gene therapy in cancer treatment: Why go nano?. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12030233"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.2147\/IJN.S355142","article-title":"Multifunctional gold nanoparticles in cancer diagnosis and treatment","volume":"17","author":"Yang","year":"2022","journal-title":"Int. J. Nanomed."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2444516","DOI":"10.1155\/2022\/2444516","article-title":"Recent Advances in the Development of Noble Metal NPs for Cancer Therapy","volume":"2022","author":"Zhao","year":"2022","journal-title":"Bioinorg. Chem. Appl."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"49581","DOI":"10.1021\/acsami.1c14275","article-title":"Bright future of gold nanoclusters in theranostics","volume":"13","author":"Deepagan","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e1704","DOI":"10.1002\/wnan.1704","article-title":"Combined cancer therapeutics\u2014Tackling the complexity of the tumor microenvironment","volume":"13","author":"Raposo","year":"2021","journal-title":"WIREs Nanomed. Nanobiotechnol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ahmad, F., Salem-Bekhit, M.M., Khan, F., Alshehri, S., Khan, A., Ghoneim, M.M., Wu, H.F., Taha, E.I., and Elbagory, I. (2022). Unique properties of surface-functionalized nanoparticles for bio-application: Functionalization mechanisms and importance in application. Nanomaterials, 12.","DOI":"10.3390\/nano12081333"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1021\/acs.molpharmaceut.9b00021","article-title":"Triggered RNAi therapy using metal inorganic nanovectors","volume":"16","author":"Leal","year":"2019","journal-title":"Mol. Pharm."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"26313","DOI":"10.1021\/jp505979e","article-title":"Targeted combinatorial therapy using gold nanostars as theranostic platforms","volume":"118","author":"Barbosa","year":"2014","journal-title":"J. Phys. Chem. C"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1186\/s12951-019-0538-3","article-title":"Combination of light-driven co-delivery of chemodrugs and plasmonic-induced heat for cancer therapeutics using hybrid protein nanocapsules","volume":"17","author":"Pardo","year":"2019","journal-title":"J. Nanobiotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"79","DOI":"10.3389\/fbioe.2017.00079","article-title":"Nanoparticles\u2014Emerging Potential for Managing Leukemia and Lymphoma","volume":"5","author":"Vinhas","year":"2017","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Schneider-Stock, R., and Ribatti, D. (2020). The CAM Assay as an Alternative In Vivo Model for Drug Testing. Handb. Exp. Pharm.","DOI":"10.1007\/164_2020_375"},{"key":"ref_41","first-page":"2633","article-title":"Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo","volume":"11","author":"Fernandes","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/s12951-017-0321-2","article-title":"Potentiating angiogenesis arrest in vivo via laser irradiation of peptide functionalised gold nanoparticles","volume":"15","author":"Pedrosa","year":"2017","journal-title":"J Nanobiotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Lipinski, A.R., Heymann, J., Meissner, C., Karlas, A., Brinkmann, V., Meyer, T.F., and Heuer, D. (2009). Rab6 and Rab11 regulate Chlamydia trachomatis development and golgin-84-dependent Golgi fragmentation. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000615"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/s41598-018-37283-2","article-title":"GLUT1 and GLUT3 involvement in anthocyanin gastric transport- Nanobased targeted approach","volume":"9","author":"Oliveira","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.omtn.2017.05.003","article-title":"Gold Nanoparticles for BCR-ABL1 Gene Silencing: Improving Tyrosine Kinase Inhibitor Efficacy in Chronic Myeloid Leukemia","volume":"7","author":"Vinhas","year":"2017","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Strober, W. (2001). Trypan blue exclusion test of cell viability. Curr. Protoc. Immunol.","DOI":"10.1002\/0471142735.ima03bs21"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1038\/nprot.2015.076","article-title":"Quantification of nascent transcription by bromouridine immunocapture nuclear run-on RT-qPCR","volume":"10","author":"Roberts","year":"2015","journal-title":"Nat. Protoc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"14230","DOI":"10.1039\/C8NR03970D","article-title":"High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles","volume":"10","author":"Longatti","year":"2018","journal-title":"Nanoscale"},{"key":"ref_51","unstructured":"Conde, J., Rosa, G., and Baptista, P.V. (2013). Gold-Nanobeacons as a Theranostic System for the Detection and Inhibition of Specific Genes. Nat. Protoc. Exch., Available online: https:\/\/www.nature.com\/protocolexchange\/protocols\/2881."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2516","DOI":"10.1016\/j.biomaterials.2012.12.015","article-title":"Gold-nanobeacons for simultaneous gene specific silencing and intracellular tracking of the silencing events","volume":"34","author":"Conde","year":"2013","journal-title":"Biomaterials"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1477-3155-8-33","article-title":"Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images","volume":"8","author":"Wang","year":"2010","journal-title":"J. Nanobiotechnol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1002\/ar.23051","article-title":"Clathrin-mediated endocytosis of gold nanoparticles in vitro","volume":"298","author":"Ng","year":"2015","journal-title":"Anat. Rec."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"O\u2019Sullivan, M.J., and Lindsay, A.J. (2020). The Endosomal Recycling Pathway-At the Crossroads of the Cell. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176074"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1006\/geno.1994.1434","article-title":"Molecular analysis of mouse Rab11b: A new type of mammalian YPT\/Rab protein","volume":"22","author":"Lai","year":"1994","journal-title":"Genomics"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1161\/ATVBAHA.115.306549","article-title":"Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function","volume":"36","author":"Yan","year":"2016","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3392","DOI":"10.1016\/j.febslet.2013.08.040","article-title":"The cytoplasmic domain of neuropilin-1 regulates focal adhesion turnover","volume":"587","author":"Seerapu","year":"2013","journal-title":"FEBS Lett."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s12935-018-0532-0","article-title":"Overexpression of Rab11-FIP2 in colorectal cancer cells promotes tumor migration and angiogenesis through increasing secretion of PAI-1","volume":"18","author":"Dong","year":"2018","journal-title":"Cancer Cell Int."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s12032-020-01447-w","article-title":"Steps in metastasis: An updated review","volume":"38","author":"Majidpoor","year":"2021","journal-title":"Med. Oncol."},{"key":"ref_61","first-page":"D682","article-title":"Ensembl 2020","volume":"48","author":"Yates","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/cdd.2009.152","article-title":"VEGF-dependent tumor angiogenesis requires inverse and reciprocal regulation of VEGFR1 and VEGFR2","volume":"17","author":"Zhang","year":"2010","journal-title":"Cell Death Differ."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"7037","DOI":"10.1038\/s41598-019-43185-8","article-title":"VEGF Receptor 1-Expressing Macrophages Recruited from Bone Marrow Enhances Angiogenesis in Endometrial Tissues","volume":"9","author":"Sekiguchi","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.1016\/j.ejca.2004.05.024","article-title":"Different signalling pathways regulate VEGF and IL-8 expression in breast cancer: Implications for therapy","volume":"40","author":"Miller","year":"2004","journal-title":"Eur. J. Cancer"},{"key":"ref_65","first-page":"900","article-title":"Aberrant p53 Expression Correlates With Expression of Vascular Endothelial Growth Factor mRNA and Interleukin-8 mRNA and Neoangiogenesis in Non\u2013Small-Cell Lung Cancer","volume":"20","author":"Yuan","year":"2002","journal-title":"J. Clin. Oncol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.humimm.2009.03.011","article-title":"Analysis of interleukin (IL)-8 expression in human astrocytomas: Associations with IL-6, cyclooxygenase-2, vascular endothelial growth factor, and microvessel morphometry","volume":"70","author":"Samaras","year":"2009","journal-title":"Hum. Immunol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1002\/ijc.10398","article-title":"Effects of pharmacologic antagonists of epidermal growth factor receptor, PI3K and MEK signal kinases on NF-\u03baB and AP-1 activation and IL-8 and VEGF expression in human head and neck squamous cell carcinoma lines","volume":"99","author":"Bancroft","year":"2002","journal-title":"Int. J. Cancer"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"5758","DOI":"10.1158\/0008-5472.CAN-13-3512","article-title":"Cellular disposal of miR23b by RAB27-dependent exosome release is linked to acquisition of metastatic properties","volume":"74","author":"Ostenfeld","year":"2014","journal-title":"Cancer Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.3892\/mmr.2013.1738","article-title":"Exosomes derived from Rab27a-overexpressing tumor cells elicit efficient induction of antitumor immunity","volume":"8","author":"Li","year":"2013","journal-title":"Mol. Med. Rep."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1016\/j.nano.2017.01.013","article-title":"Smuggling gold nanoparticles across cell types\u2014A new role for exosomes in gene silencing","volume":"13","author":"Pereira","year":"2017","journal-title":"Nanomedicine"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1535750","DOI":"10.1080\/20013078.2018.1535750","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Witwer","year":"2018","journal-title":"J. Extracell. 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