{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:18:01Z","timestamp":1790039881906,"version":"4.0.1"},"reference-count":57,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T00:00:00Z","timestamp":1678924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation and Tomsk region","award":["22-13-20032"],"award-info":[{"award-number":["22-13-20032"]}]},{"name":"Russian Science Foundation and Tomsk region","award":["1073"],"award-info":[{"award-number":["1073"]}]},{"name":"the \u201cInvestigadoras e investigadores por M\u00e9xico\u201d CONACyT program","award":["22-13-20032"],"award-info":[{"award-number":["22-13-20032"]}]},{"name":"the \u201cInvestigadoras e investigadores por M\u00e9xico\u201d CONACyT program","award":["1073"],"award-info":[{"award-number":["1073"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Introduction: Silver nanoparticles (AgNPs) have a wide range of bioactivity, which is highly dependent on particle size, shape, stabilizer, and production method. Here, we present the results of studies of AgNPs cytotoxic properties obtained by irradiation treatment of silver nitrate solution and various stabilizers by accelerating electron beam in a liquid medium. Methods: The results of studies of morphological characteristics of silver nanoparticles were obtained by transmission electron microscopy, UV-vis spectroscopy, and dynamic light scattering measurements. MTT test, alamar blue test, flow cytometry, and fluorescence microscopy were used to study the anti-cancer properties. As biological objects for standard tests, adhesive and suspension cell cultures of normal and tumor origin, including prostate cancer, ovarian cancer, breast cancer, colon cancer, neuroblastoma, and leukemia, were studied. Results: The results showed that the silver nanoparticles obtained by irradiation with polyvinylpyrrolidone and collagen hydrolysate are stable in solutions. Samples with different stabilizers were characterized by a wide average size distribution from 2 to 50 nm and low zeta potential from \u22127.3 to +12.4 mV. All AgNPs formulations showed a dose-dependent cytotoxic effect on tumor cells. It has been established that the particles obtained with the combination of polyvinylpyrrolidone\/collagen hydrolysate have a relatively more pronounced cytotoxic effect in comparison to samples stabilized with only collagen or only polyvinylpyrrolidone. The minimum inhibitory concentrations for nanoparticles were less than 1 \u03bcg\/mL for various types of tumor cells. It was found that neuroblastoma (SH-SY5Y) is the most susceptible, and ovarian cancer (SKOV-3) is the most resistant to the action of silver nanoparticles. The activity of the AgNPs formulation prepared with a mixture of PVP and PH studied in this work was higher that activity of other AgNPs formulations reported in the literature by about 50 times. Conclusions: The results indicate that the AgNPs formulations synthesized with an electron beam and stabilized with polyvinylpyrrolidone and protein hydrolysate deserve deep study for their further use in selective cancer treatment without harming healthy cells in the patient organism.<\/jats:p>","DOI":"10.3390\/pharmaceutics15030962","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T04:10:48Z","timestamp":1679026248000},"page":"962","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A Comparative Study of Cancer Cells Susceptibility to Silver Nanoparticles Produced by Electron Beam"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4374-6422","authenticated-orcid":false,"given":"Evgenii V.","family":"Plotnikov","sequence":"first","affiliation":[{"name":"Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, Russia"},{"name":"Mental Health Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk 634014, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria S.","family":"Tretayakova","sequence":"additional","affiliation":[{"name":"Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, Russia"},{"name":"Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk 634009, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Garibo-Ru\u00edz","sequence":"additional","affiliation":[{"name":"Nanoscience and Nanotechnology Center (CNyN), Campus Ensenada, National Autonomous University of Mexico (UNAM), Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7094-1912","authenticated-orcid":false,"given":"Ana G.","family":"Rodr\u00edguez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Nanoscience and Nanotechnology Center (CNyN), Campus Ensenada, National Autonomous University of Mexico (UNAM), Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9034-4733","authenticated-orcid":false,"given":"Alexey N.","family":"Pestryakov","sequence":"additional","affiliation":[{"name":"Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2595-7939","authenticated-orcid":false,"given":"Yanis","family":"Toledano-Maga\u00f1a","sequence":"additional","affiliation":[{"name":"Escuela de Ciencias de la Salud, Universidad Aut\u00f3noma de Baja California, Ensenada 22890, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Bogdanchikova","sequence":"additional","affiliation":[{"name":"Nanoscience and Nanotechnology Center (CNyN), Campus Ensenada, National Autonomous University of Mexico (UNAM), Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1007\/s00231-021-03161-3","article-title":"Heat and mass transfer in the hyperthermia cancer treatment by magnetic nanoparticles","volume":"58","author":"Darvishi","year":"2022","journal-title":"Heat Mass Transf."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s11671-018-2457-x","article-title":"Dependence of Nanoparticle Toxicity on Their Physical and Chemical Properties","volume":"13","author":"Sukhanova","year":"2018","journal-title":"Nanoscale Res. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1007\/s10856-017-5902-y","article-title":"Size and shape-dependent cytotoxicity profile of gold nanoparticles for biomedical applications","volume":"28","author":"Malankowska","year":"2017","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"375496","DOI":"10.1155\/2012\/375496","article-title":"Effect of Size, Shape, and Surface Modification on Cytotoxicity of Gold Nanoparticles to Human HEp-2 and Canine MDCK Cells","volume":"2012","author":"Zhang","year":"2012","journal-title":"J. Nanomater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"866","DOI":"10.3762\/bjnano.11.72","article-title":"Key for crossing the BBB with nanoparticles: The rational design","volume":"11","author":"Lombardo","year":"2020","journal-title":"Beilstein J. Nanotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ajdary, M., Moosavi, M.A., Rahmati, M., Falahati, M., Mahboubi, M., Mandegary, A., Jangjoo, S., Mohammadinejad, R., and Varma, R.S. (2018). Health Concerns of Various Nanoparticles: A Review of Their in Vitro and in Vivo Toxicity. Nanomaterials, 8.","DOI":"10.20944\/preprints201806.0273.v1"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1093\/toxsci\/kfv068","article-title":"Mitochondrial Toxicity of Cadmium Telluride Quantum Dot Nanoparticles in Mammalian Hepatocytes","volume":"146","author":"Nguyen","year":"2015","journal-title":"Toxicol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1016\/j.fsi.2018.10.007","article-title":"Silver nanoparticles enhance survival of white spot syndrome virus infected Penaeus vannamei shrimps by activation of its immunological system","volume":"84","author":"Barraza","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8894","DOI":"10.3390\/molecules16108894","article-title":"Silver nanoparticles as potential antiviral agents","volume":"16","author":"Galdiero","year":"2011","journal-title":"Molecules"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ratan, Z.A., Mashrur, F.R., Chhoan, A.P., Shahriar, S.M., Haidere, M.F., Runa, N.J., Kim, S., Kweon, D.H., Hosseinzadeh, H., and Cho, J.Y. (2021). Silver Nanoparticles as Potential Antiviral Agents. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13122034"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1002\/cmdc.200900049","article-title":"Further evidence of the anti-inflammatory effects of silver nanoparticles","volume":"4","author":"Wong","year":"2009","journal-title":"ChemMedChem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"035008","DOI":"10.1088\/2043-6262\/5\/3\/035008","article-title":"Antioxidant and anti-inflammatory activities of silver nanoparticles biosynthesized from aqueous leaves extracts of four Terminalia species","volume":"5","year":"2014","journal-title":"Adv. Nat. Sci. Nanosci. Nanotechnol."},{"key":"ref_13","first-page":"1559325817744945","article-title":"Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System","volume":"15","author":"Bogdanchikova","year":"2017","journal-title":"Dose Response"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1002\/jat.2780","article-title":"Silver nanoparticles: A brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles","volume":"32","author":"Seabra","year":"2012","journal-title":"J. Appl. Toxicol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jare.2017.10.008","article-title":"A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives","volume":"9","author":"Akter","year":"2018","journal-title":"J. Adv. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s11671-018-2450-4","article-title":"Recent advances in synthetic methods and applications of silver nanostructures","volume":"13","author":"Zhang","year":"2018","journal-title":"Nanoscale Res. Lett."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bouafia, A., Laouini, S.E., Ahmed, A.S.A., Soldatov, A.V., Algarni, H., Feng Chong, K., and Ali, G.A.M. (2021). The Recent Progress on Silver Nanoparticles: Synthesis and Electronic Applications. Nanomaterials, 11.","DOI":"10.3390\/nano11092318"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100205","DOI":"10.1016\/j.crgsc.2021.100205","article-title":"Conventional and green methods of synthesis of silver nanoparticles and their antimicrobial properties","volume":"4","author":"Vishwanath","year":"2021","journal-title":"Curr. Res. Green Sustain. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"74","DOI":"10.4028\/www.scientific.net\/AMR.872.74","article-title":"Properties of Silver Nanoparticles Prepared by the Electric Spark Dispersion Method","volume":"872","author":"Zhuravkov","year":"2014","journal-title":"Adv. Mater. Res."},{"key":"ref_20","unstructured":"Burmistrov, V.A., Burmistrov, A.V., Burmistrov, I.V., Burmistrov, A.V., Pestrjakov, A.N., Odegova, G.V., and Bogdanchikova, N.E. (2016). Method of Producing Colloidal Silver Nanoparticles. (RU 2,602,534), Patent."},{"key":"ref_21","unstructured":"Burmistrov, V.A., Burmistrov, A.V., Burmistrov, I.V., Burmistrov, A.V., Pestrjakov, A.N., Odegova, G.V., and Bogdanchikova, N.E. (2018). Method for Silver Proteinate Production. (RU 2,646,105), Patent."},{"key":"ref_22","unstructured":"(2018, March 30). Vector-Vita. Available online: http:\/\/vector-vita.narod.ru\/links.html."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Almanza-Reyes, H., Moreno, S., Plascencia-L\u00f3pez, I., Alvarado-Vera, M., Patr\u00f3n-Romero, L., Borrego, B., Reyes-Escamilla, A., Valencia-Manzo, D., Brun, A., and Pestryakov, A. (2021). Evaluation of silver nanoparticles for the prevention of SARS-CoV-2 infection in health workers: In vitro and in vivo. PLoS ONE, 16.","DOI":"10.1101\/2021.05.20.21256197"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Gastelum-Leyva, F., Pena-Jasso, A., Alvarado-Vera, M., Plascencia-L\u00f3pez, I., Patr\u00f3n-Romero, L., Loera-Casta\u00f1eda, V., G\u00e1ndara-Mireles, J.A., Lares-Asseff, I., Leal-\u00c1vila, M.\u00c1., and Alvelais-Palacios, J.A. (2022). Evaluation of the Efficacy and Safety of Silver Nanoparticles in the Treatment of Non-Neurological and Neurological Distemper in Dogs: A Randomized Clinical Trial. Viruses, 14.","DOI":"10.3390\/v14112329"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6146","DOI":"10.1039\/C9RA09018E","article-title":"Argovit\u2122 silver nanoparticles to fight Huanglongbing disease in Mexican limes (Citrus aurantifolia Swingle)","volume":"10","author":"Pestryakov","year":"2020","journal-title":"RSC Adv."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Luna-V\u00e1zquez-G\u00f3mez, R., Arellano-Garc\u00eda, M.E., Toledano-Maga\u00f1a, Y., Garc\u00eda-Ramos, J.C., Radilla-Ch\u00e1vez, P., Salas-Vargas, D.S., Casillas-Figueroa, F., Ruiz-Ruiz, B., Pestryakov, A., and Bogdanchikova, N. (2022). Bell Shape Curves of Hemolysis Induced by Silver Nanoparticles: Review and Experimental Assay. Nanomaterials, 12.","DOI":"10.3390\/nano12071066"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"12005","DOI":"10.1021\/acsomega.0c00149","article-title":"Cytokinesis-Block Micronucleus Assay Using Human Lymphocytes as a Sensitive Tool for Cytotoxicity\/Genotoxicity Evaluation of AgNPs","volume":"5","author":"Pestryakov","year":"2020","journal-title":"ACS Omega"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Cruz-Ram\u00edrez, O.U., Valenzuela-Salas, L.M., Blanco-Salazar, A., Rodr\u00edguez-Arenas, J.A., Mier-Maldonado, P.A., Garc\u00eda-Ramos, J.C., Bogdanchikova, N., Pestryakov, A., and Toledano-Maga\u00f1a, Y. (2021). Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]\/[PVP] Ratio. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13071000"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Valenzuela-Salas, L.M., Blanco-Salazar, A., Perrusqu\u00eda-Hern\u00e1ndez, J.D., Nequiz-Avenda\u00f1o, M., Mier-Maldonado, P.A., Ruiz-Ruiz, B., Campos-Gallegos, V., Arellano-Garc\u00eda, M.E., Garc\u00eda-Ramos, J.C., and Pestryakov, A. (2021). New Protein-Coated Silver Nanoparticles: Characterization, Antitumor and Amoebicidal Activity, Antiproliferative Selectivity, Genotoxicity, and Biocompatibility Evaluation. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13010065"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.jmbbm.2012.09.011","article-title":"Effects of gamma ray and electron beam irradiation on the mechanical, thermal, structural and physicochemical properties of poly (ether-block-amide) thermoplastic elastomers","volume":"17","author":"Murray","year":"2013","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e03423","DOI":"10.1016\/j.heliyon.2020.e03423","article-title":"L-cysteine modified silver nanoparticles for selective and sensitive colorimetric detection of vitamin B1 in food and water samples","volume":"6","author":"Khalkho","year":"2020","journal-title":"Heliyon"},{"key":"ref_32","first-page":"153","article-title":"Silver Nanoparticles as Fluorescent Probes: New Approach for Bioimaging","volume":"2","author":"Singh","year":"2013","journal-title":"Int. J. Sci. Technol. Res."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Nimesh, S., Chandra, R., and Gupta, N. (2017). Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids, Woodhead Publishing.","DOI":"10.1016\/B978-0-08-100557-6.00001-8"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4675","DOI":"10.18632\/oncotarget.22563","article-title":"Silver nanoparticles of different sizes induce a mixed type of programmed cell death in human pancreatic ductal adenocarcinoma","volume":"9","author":"Zielinska","year":"2018","journal-title":"Oncotarget"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s12989-021-00430-1","article-title":"The mechanism of cell death induced by silver nanoparticles is distinct from silver cations","volume":"18","author":"Rohde","year":"2021","journal-title":"Part. Fibre Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.2147\/IJN.S152237","article-title":"Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis","volume":"13","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1002\/jat.2792","article-title":"Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues","volume":"33","author":"Kim","year":"2013","journal-title":"J. Appl. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/978-1-62703-640-5_2","article-title":"Considerations for the use of SH-SY5Y neuroblastoma cells in neurobiology","volume":"1078","author":"Kovalevich","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.toxlet.2018.01.004","article-title":"Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function","volume":"285","author":"Li","year":"2018","journal-title":"Toxicol. Lett."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.msec.2014.08.019","article-title":"Sunflower oil mediated biomimetic synthesis and cytotoxicity of monodisperse hexagonal silver nanoparticles","volume":"44","author":"Thakore","year":"2014","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"259765","DOI":"10.1155\/2014\/259765","article-title":"Assessment of cellular responses after short- and long-term exposure to silver nanoparticles in human neuroblastoma (SH-SY5Y) and astrocytoma (D384) cells","volume":"2014","author":"Coccini","year":"2014","journal-title":"Sci. World J."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Gurunathan, S., Jeyaraj, M., Kang, M.H., and Kim, J.H. (2019). Mitochondrial Peptide Humanin Protects Silver Nanoparticles-Induced Neurotoxicity in Human Neuroblastoma Cancer Cells (SH-SY5Y). Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20184439"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"535796","DOI":"10.1155\/2013\/535796","article-title":"Cytotoxicity of biologically synthesized silver nanoparticles in MDA-MB-231 human breast cancer cells","volume":"2013","author":"Gurunathan","year":"2013","journal-title":"BioMed Res. Int."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1177\/0960327116675206","article-title":"Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines","volume":"36","author":"Gonzalez","year":"2017","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"319","DOI":"10.3109\/17435390.2010.483745","article-title":"Impact of silver nanoparticles on human cells: Effect of particle size","volume":"4","author":"Liu","year":"2010","journal-title":"Nanotoxicology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.msec.2015.04.038","article-title":"Bio-synthesis of silver nanoparticles using Potentilla fulgens Wall. ex Hook. and its therapeutic evaluation as anticancer and antimicrobial agent","volume":"53","author":"Mittal","year":"2015","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Gurunathan, S., Qasim, M., Park, C., Yoo, H., Kim, J.H., and Hong, K. (2018). Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19082269"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.cbi.2018.07.028","article-title":"Thermal Co-reduction engineered silver nanoparticles induce oxidative cell damage in human colon cancer cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis","volume":"295","author":"Dasgupta","year":"2018","journal-title":"Chem.-Biol. Interact."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Khedr, A.I.M., Goda, M.S., Farrag, A.F.S., Nasr, A.M., Swidan, S.A., Nafie, M.S., Abdel-Kader, M.S., Badr, J.M., and Abdelhameed, R.F.A. (2022). Silver Nanoparticles Formulation of Flower Head\u2019s Polyphenols of Cynara scolymus L.: A Promising Candidate against Prostate (PC-3) Cancer Cell Line through Apoptosis Activation. Molecules, 27.","DOI":"10.3390\/molecules27196304"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Khedr, A.I.M., Farrag, A.F.S., Nasr, A.M., Swidan, S.A., Nafie, M.S., Abdel-Kader, M.S., Goda, M.S., Badr, J.M., and Abdelhameed, R.F.A. (2022). Comparative Estimation of the Cytotoxic Activity of Different Parts of Cynara scolymus L.: Crude Extracts versus Green Synthesized Silver Nanoparticles with Apoptotic Investigation. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14102185"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"127004","DOI":"10.1016\/j.jtemb.2022.127004","article-title":"Cytotoxicity of nanomixture: Combined action of silver and plastic nanoparticles on immortalized human lymphocytes","volume":"73","author":"Krce","year":"2022","journal-title":"J. Trace Elem. Med. Biol. Organ Soc. Miner. Trace Elem. (GMS)"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3650","DOI":"10.4238\/2015.April.17.15","article-title":"Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-\u03b1 production via MAPK pathway in leukemic cell lines","volume":"14","author":"Parnsamut","year":"2015","journal-title":"Genet. Mol. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5107485","DOI":"10.1155\/2017\/5107485","article-title":"Heterogeneous Responses of Ovarian Cancer Cells to Silver Nanoparticles as a Single Agent and in Combination with Cisplatin","volume":"2017","author":"Fahrenholtz","year":"2017","journal-title":"J. Nanomater."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1007\/s42452-020-03699-7","article-title":"Biogenesis of silver nanoparticles using leaf extracts of Asparagus racemosus and Sophora interrupta: Structure characterization, antibacterial and anticancer studies","volume":"2","author":"Satyanarayana","year":"2020","journal-title":"SN Appl. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Gurunathan, S., Qasim, M., Park, C., Yoo, H., Choi, D.Y., Song, H., Park, C., Kim, J.-H., and Hong, K. (2018). Cytotoxicity and Transcriptomic Analysis of Silver Nanoparticles in Mouse Embryonic Fibroblast Cells. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113618"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1515\/ntrev-2021-0066","article-title":"An overview of methods for production and detection of silver nanoparticles, with emphasis on their fate and toxicological effects on human, soil, and aquatic environment","volume":"10","author":"Ghobashy","year":"2021","journal-title":"Nanotechnol. Rev."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Luna-V\u00e1zquez-G\u00f3mez, R., Arellano-Garc\u00eda, M.E., Garc\u00eda-Ramos, J.C., Radilla-Ch\u00e1vez, P., Salas-Vargas, D.S., Casillas-Figueroa, F., Ruiz-Ruiz, B., Bogdanchikova, N., and Pestryakov, A. (2021). Hemolysis of Human Erythrocytes by Argovit\u2122 AgNPs from Healthy and Diabetic Donors: An In Vitro Study. Materials, 14.","DOI":"10.3390\/ma14112792"}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/15\/3\/962\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:56:39Z","timestamp":1760122599000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/15\/3\/962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,16]]},"references-count":57,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["pharmaceutics15030962"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics15030962","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,16]]}}}