{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T12:35:58Z","timestamp":1773232558789,"version":"3.50.1"},"reference-count":61,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o Amaz\u00f4nia Paraense de Amparo \u00e0 Pesquisa\u2014FAPESPA","award":["036\/2021"],"award-info":[{"award-number":["036\/2021"]}]},{"name":"Funda\u00e7\u00e3o Amaz\u00f4nia Paraense de Amparo \u00e0 Pesquisa\u2014FAPESPA","award":["APQ-01242-17"],"award-info":[{"award-number":["APQ-01242-17"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais\u2014FAPEMIG","award":["036\/2021"],"award-info":[{"award-number":["036\/2021"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais\u2014FAPEMIG","award":["APQ-01242-17"],"award-info":[{"award-number":["APQ-01242-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Malaria is an infectious and parasitic disease caused by protozoa of the genus Plasmodium, which affects millions of people in tropical and subtropical areas. Recently, there have been multiple reports of drug resistance in Plasmodium populations, leading to the search for potential new active compounds against the parasite. Thus, we aimed to evaluate the in vitro antiplasmodial activity and cytotoxicity of the hydroalcoholic extract of Juc\u00e1 (Libidibia ferrea) in serial concentrations. Juc\u00e1 was used in the form of a freeze-dried hydroalcoholic extract. For the cytotoxicity assay, the(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method with the WI-26VA4 human cell line was used. For the antiplasmodial activity, Plasmodium falciparum synchronized cultures were treated with serial concentrations (0.2 to 50 \u03bcg\/mL) of the Juc\u00e1 extract. In terms of the chemical composition of the Juc\u00e1 extract, gas chromatography coupled to mass spectrometry measurements revealed the main compounds as ellagic acid, valoneic acid dilactone, gallotannin, and gallic acid. The Juc\u00e1 hydroalcoholic extract did not show cytotoxic activity per MTT, with an IC50 value greater than 100 \u00b5g\/mL. Regarding the antiplasmodial activity, the Juc\u00e1 extract presented an IC50 of 11.10 \u00b5g\/mL with a selective index of nine. Because of its antiplasmodial activity at the tested concentrations and low toxicity, the Juc\u00e1 extract is presented as a candidate for herbal medicine in the treatment of malaria. To the best of our knowledge, this is the first report of antiplasmodial activity in Juc\u00e1.<\/jats:p>","DOI":"10.3390\/pharmaceutics15041162","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T03:59:55Z","timestamp":1680753595000},"page":"1162","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antiplasmodial Activity of Hydroalcoholic Extract from Juc\u00e1 (Libidibia ferrea) Pods"],"prefix":"10.3390","volume":"15","author":[{"given":"Francisco Fl\u00e1vio Vieira de","family":"Assis","sequence":"first","affiliation":[{"name":"Laboratory of Animal Health, LARSANA, Federal University of Western Par\u00e1, UFOPA, Santar\u00e9m 68040-255, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5989-3702","authenticated-orcid":false,"given":"Jos\u00e9 Sousa de","family":"Almeida Junior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Farmacologia Experimental, Universidade Federal do Oeste do Par\u00e1, UFOPA, Santar\u00e9m 68040-255, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia Mara Pires","family":"Moraes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Farmacologia Experimental, Universidade Federal do Oeste do Par\u00e1, UFOPA, Santar\u00e9m 68040-255, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2939-7780","authenticated-orcid":false,"given":"Fernando de Pilla","family":"Varotti","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa em Qu\u00edmica Biol\u00f3gica (NQBio), Universidade Federal de S\u00e3o Jo\u00e3o Del Rei, Campus Centro-Oeste, Av. Sebasti\u00e3o G. Coelho, 400, Chanadour, Divin\u00f3polis 35501-296, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Castilho","family":"Moraes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Farmacologia Experimental, Universidade Federal do Oeste do Par\u00e1, UFOPA, Santar\u00e9m 68040-255, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1763-3715","authenticated-orcid":false,"given":"Adilson","family":"Sartoratto","sequence":"additional","affiliation":[{"name":"Centro Pluridisciplinar de Pesquisas Qu\u00edmicas, Biol\u00f3gicas e Agr\u00edcolas, Universidade de Campinas\u2014UNICAMP, Campinas 13148-218, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waldiney Pires","family":"Moraes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Farmacologia Experimental, Universidade Federal do Oeste do Par\u00e1, UFOPA, Santar\u00e9m 68040-255, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6742-3652","authenticated-orcid":false,"given":"Antonio Humberto Hamad","family":"Minervino","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Health, LARSANA, Federal University of Western Par\u00e1, UFOPA, Santar\u00e9m 68040-255, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1590\/S0104-07072010000300016","article-title":"User Adherence and Reactions to Malaria Treatment: Implications for Health Education","volume":"19","author":"Reiners","year":"2010","journal-title":"Texto E Contexto Enferm."},{"key":"ref_2","unstructured":"WHO (2021). 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